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  • Pisum
  • Springer  (25)
  • Annual Reviews
  • Springer Science + Business Media
  • 1995-1999  (2)
  • 1980-1984  (13)
  • 1975-1979  (10)
Collection
Keywords
Publisher
  • Springer  (25)
  • Annual Reviews
  • Springer Science + Business Media
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Effect of galactoglucomannan-derived oligosaccharides on elongation growth of pea and spruce stem segments stimulated by auxin (1995)
    Springer
    Planta 196 (1995), S. 420-424 
    Details
    ISSN: 1432-2048
    Keywords: 2,4-Dichlorophenoxyacetic acid ; Elongation growth ; Galactoglucomannan-derived oligosaccharides ; Picea ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Galactoglucomannan-derived oligosaccharides (GGMOs) (degree of polymerization 4–8) isolated from the wood of poplar (Populus monilifera Ait.) were shown to be inhibitors of the 2,4-dichlorophenoxyacetic acid-stimulated elongation growth of pea (Pisum sativum L. cv. Tyrkys) and spruce [Picea abies (L.) Karst] stem segments. A dependence on the concentration of GGMOs (between 10-5-10-10M) as well as plant species was ascertained. Pea stem segments were much more sensitive (10-10M) than spruce (10-8M). The GGMOs did not exhibit toxicity even at high concentrations and during long-term bioassays. The timing of the action of GGMOs and auxin in the growth process was also studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203638
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  • 2
    Electronic Resource
    Electronic Resource
    Identification by combined gas chromatography-mass spectrometry of phaseic acid and dihydrophaseic acid and characterization of further abscisic acid metabolites in pea seedlings (1979)
    Springer
    Planta 147 (1979), S. 168-173 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid metabolites ; Dihydrophaseic acid ; Phaseic acid ; Pisum ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seven day old seedlings of Pisum sativum L., cv. Kleine Rheinländerin, were wilted for 3 days. After partially removing the roots, they were rewatered and at the same time radioactive abscisic acid([1-14C]ABA, spec. activity 1.7·108d s-1mmol-1) was applied for 1 h via the xylem of the roots. After 24 h, 4 days, and 12 days the seedlings were extracted and the metabolites of ABA were analyzed by means of thin-layer and gas chromatography in combination with mass spectrometry, autoradiography, and scintillation counting. Phaseic acid (PA) and dihydrophaseic acid (DPA) were identified as metabolites of ABA. The presence of another ABA-metabolite was also demonstrated. From its mass spectrum it has been postulated that this metabolite is 4′-desoxy-ABA. In addition to these substances, several other metabolites, which are more polar than ABA and its known degradation products, were present in the seedlings. The quantity and number of these unknown metabolites increased with time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389520
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  • 3
    Electronic Resource
    Electronic Resource
    Role of ATP in nitrite reduction in roots of wheat and pea (1981)
    Springer
    Planta 152 (1981), S. 234-238 
    Details
    ISSN: 1432-2048
    Keywords: ATP and nitrite reduction ; Glucose-6-phosphate ; Nitrite reduction ; Pisum ; Plastids, nitrite reduction ; Root, plastids ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Excised wheat (Triticum aestivum L.) and field pea (Pisum arvense L.) roots, incubated under anaerobic conditions or in the presence of uncouplers of oxidative phosphorylation [2,4-dinitrophenol (DNP), carbonylcyanide-m-chlorophenylhydrazone, pentachlorophenol] accumulated nitrite as a result of an inhibition of nitrite reduction. In isolated root plastids, nitrite reduction was dependent on a supply of glucose-6-phosphate (G6P) and did not require ATP. The estimated Km value for glucose 6-phosphate was 1.25 mM. Glucose and fructose-1,6-diphosphate were ineffective substrates for nitrate reduction. Anaerobic conditions and treatment with DNP, which would result in a cessation of ATP production by the mitochondria and a stimulation of glycolysis via the “Pasteur effect”, were shown to decrease the G6P content of excised roots of wheat and pea. A negative correlation was observed between the level of G6P and nitrite accumulation on root tissues. It is proposed that an interruption in the supply of G6P to the root plastid under these conditions would result in an inhibition of nitrite reduction leading to nitrite accumulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385149
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  • 4
    Electronic Resource
    Electronic Resource
    Picosecond fluorescence decay studies on water-stressed pea leaves: energy transfer and quenching processes in photosystem 2 (1997)
    Springer
    Photosynthetica 34 (1997), S. 97-106 
    Details
    ISSN: 1573-9058
    Keywords: chlorophyll ; DCMU ; exciton-radical pair equilibrium model ; NH2OH ; photosynthesis ; Pisum ; quinones ; relative air humidity ; wilting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Detached leaves of pea (Pisum sativum) were submitted to water stress at different relative air humidities. The photosynthetic activity of photosystem 2 (PS2) was monitored by time-resolved picosecond chlorophyll (Chl) fluorescence spectroscopy. In the first days the well-known fast Chl fluorescence decay was observed which indicated high PS2 activity. After a few days the average fluorescence decay time τm reached a maximum, depending on the wilting conditions, but always at a relative loss of leaf mass of 80%. After this maximum, τm decreased within a few hours, the fluorescence decay became similar to that one of an intact leaf, but an additional fluorescence decay component with a lifetime of 3.6 ns appeared. At first the primary quinone QA was reduced due to inhibition of the electron transfer to the secondary quinone QB. Simultaneously, water deficiency caused an electron lack at the oxidizing site of PS2. This disabled the primary electron donor of PS2, tyrosine Z, from reducing the oxidized reaction centre of PS2 (P680+). Thus a recombination of P680+-pheophytin-QA- took place, and the energy was lost as heat. With further water stress, QA was decoupled from PS2. The new fluorescence decay component could therefore be assigned to energetically decoupled antenna complexes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006867902382
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  • 5
    Electronic Resource
    Electronic Resource
    Evidence for carrier-mediated transport of L-leucine into isolated pea (Pisum sativum L.) chloroplasts (1977)
    Springer
    Planta 136 (1977), S. 147-151 
    Details
    ISSN: 1432-2048
    Keywords: Carrier ; mediated transport ; Chloroplast ; Leucine ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The uptake of leucine into isolated, intact, pea chloroplasts was investigated using the silicone oil centrifugation technique. The internal: external ratio of leucine exceeded unity at low external leucine concentrations. Uptake of leucine at different external concentrations showed passive diffusion and carrier-mediated transport components. Competition for uptake was shown between leucine and isoleucine but not between leucine and glycine. Rates of diffusion of leucine were found to be low compared with glycine, however, fast carrier-mediated transport of leucine assumed more importance at physiological concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396190
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  • 6
    Electronic Resource
    Electronic Resource
    Concanavalin A-mediated agglutination of plant plastids (1978)
    Springer
    Planta 138 (1978), S. 35-39 
    Details
    ISSN: 1432-2048
    Keywords: Agglutination ; Chloroplasts ; Concanavalin A ; Cucumis ; Pisum ; Pyrus ; Proplastids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cucumber (Cucumis sativus L.) and pear (Pyrus domestica Medik.) fruit proplastids, and pea (Pisum sativum L., cv. Meteor) leaf chloroplasts, extracted by osmotic rupture of protoplasts isolated after degradation of the cell walls by cellulase and pectinase, agglutinated in the presence of Con A. Agglutination of cucumber proplastids was inhibited by anti-Con A and by methyl α D-gluco/manno pyranosides but not by methyl α D-galactopyranoside. Fluorescein isothiocyanate-conjugated Con A (FITC-Con A) rendered agglutinated clumps fluorescent. If cellulase was omitted from the macerating medium, Con A-mediated agglutination did not occur even if proplatids were subsequently incubated with cellulase. Proplastids and chloroplasts extracted by conventional mechanical disruption methods were not agglutinated by Con A and did not acquire fluorescence with FITC-Con A. However, cucumber proplastids so extracted could be agglutinated by Con A if incubated with cellulase after preparation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392911
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  • 7
    Electronic Resource
    Electronic Resource
    The initiation of legumin synthesis in immature embryos of Pisum sativum L. grown in vivo and in vitro (1980)
    Springer
    Planta 149 (1980), S. 454-460 
    Details
    ISSN: 1432-2048
    Keywords: Embryo culture ; Enzyme-linked immunosorbent assay (ELISA) ; Legumin ; Pisum ; Protein (storage) ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A highly sensitive immunoassay has been used for the detection of a major storage protein, legumin, in embryos of Pisum sativum L.; with this technique nanogram quantities could be measured. In the two varieties tested, legumin could be detected in embryos in vivo, when they had attained a fresh weight of 2·10-3 g and 3·10-3 g, respectively. Contrary to earlier claims, embryos cultured in vitro were shown to be capable of initiating legumin synthesis. This capacity to initiate legumin synthesis was confirmed by two-dimensional isoelectric focusing-electrophoresis and fluorography; embryos harvested before initiation of legumin synthesis and cultured in radioactive medium were shown to have synthesized legumin subunits. The amounts of legumin and total protein synthesized per unit fresh weight were consistently greater in vitro than in equivalent embryos grown in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385747
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  • 8
    Electronic Resource
    Electronic Resource
    The influence of small direct electric currents on the transport of auxin in intact plants (1980)
    Springer
    Planta 150 (1980), S. 431-434 
    Details
    ISSN: 1432-2048
    Keywords: Auxin transport ; Electric current ; Pisum ; Potential gradients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When a d.c. potential of 9.0 V was applied to the stem of intact pea seedlings (Pisum sativum L. cv. Meteor and cv. Alderman) via 10 mM KCl-soaked filter paper electrodes placed ca. 50 mm apart the stem passed a steady current of 15–20 μA (resistance ca. 100 kΩ cm-1). The basipetal transport of [1-14C]IAA applied to the apical bud was completely inhibited over the portion of the stem through which current flowed and 14C-labelled compounds accumulated in the vicinity of the upper electrode. The inhibition of transport was independent of the polarity of the applied potential. The basipetal transport of IAA in the stem above the electrode was not affected. Labelled auxin accumulated at the upper electrode both as unchanged IAA and as a compound tentatively identified as indol-3yl-acetyl aspartic acid (IAAsp). These compounds were only slowly remobilised when the current was interrupted. However, the ability of the transport system to move freshly-applied IAA was rapidly and fully restored when the potential was removed. No injury to the plant was detected after maintaining a current flow for up to 72 h. No leakage of 14C-labelled compounds into the KCl solution bathing the electrodes was detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390181
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  • 9
    Electronic Resource
    Electronic Resource
    Ultrastructural organization of chloroplast membranes in mutants of Pisum sativum L. with impaired activity in the photosystems (1981)
    Springer
    Planta 151 (1981), S. 512-524 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast (membrane ultrastructure) ; Mutants (chloroplast structure) ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ultrastructural organization and the photosynthesis reactions of chloroplast membranes were studied in three lethal mutants of Pisum sativum, Chl-1, Chl-19 and Chl-5, all lacking the capacity to evolve oxygen. The rates of 2,6-dichloroindophenol reduction, delayed fluorescence and electron-spin-resonance signal 1 indicate that Chl-1 and Chl-19 have an impaired activity in photosystem II (PS II), while in Chl-5 the electron transport is blocked between PS I and the reactions of CO2 fixation. Ultrathin sectioning demonstrates the presence of giant grana in the chloroplasts of Chl-1 and Chl-19, while the chloroplast structure of the Chl-5 is very similar to that of the wild-type. The grana of the Chl-19 mutant contain large multilamellar regions of tightly packed membranes. When the chloroplast membranes were studied by freeze-fracture, the exoplasmic and protoplasmic fracture faces (EF and PF, respectively) in both stacked and unstacked membranes were found to show large differences in particle concentrations and relative population area (per μm2), and also in particle size distribution, between all mutant chloroplast membranes and the wild-type. A close correlation between increasing kmt (ratio of particle concentrations on PF/EF) and PS II activity was observed. The differences in particle concentrations on both fracture faces in different regions of the intact chloroplast membranes of the wild-type are the consequence of a rearrangement of existing membrane components by lateral particle movements since quantitative measurements demonstrate almost complete conservation of intramembrane particles in number and size during the stacking of stroma thylakoid membranes. The results indicating particle movements strongly support the concept that the chloroplast membranes have a highly dynamic structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387428
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  • 10
    Electronic Resource
    Electronic Resource
    Characterisation of the storage protein subunits synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.) (1980)
    Springer
    Planta 148 (1980), S. 49-56 
    Details
    ISSN: 1432-2048
    Keywords: Legumin ; Pisum ; Protien synthesis ; RNA ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Evidence is presented to show that legumin, the major storage protein in Pisum, is synthesised in vitro by the wheat germ and reticulocyte lysate systems, from polyribosomes and mRNA isolated from developing pea seeds. While legumin isolated from mature pea seeds consists of 40,000 and 20,000 MW subunits, the in vitro legumin is synthesised as a 60,000 MW precursor consisting of covalently linked 40,000 and 20,000 MW subunits. The implications of these findings are discussed in relationship to studies with other systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385441
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