ISSN:
1573-8221
Keywords:
nitric oxide
;
L-glutamate receptor
;
granule cells
;
cerebellum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Stimulation of L-glutamate receptors (GluRs) is thought to produce neuron death through the elevation of free intracellular Ca2+ levels, leading to activation of Ca2+/calmodulin-dependent synthesis of cytotoxic amounts of NO·. In the present study, NO synthase activation mediated by mGluR stimulation is investigated in primary cultures of granule cells (CGrC). It is found that a selective agonist of mGluRs, DL-1-aminocyclopentane-trans-1,3-dicarboxylic acid (ACPD), raises both the cGMP and nitrite (NO2 −) levels, which are used as a biochemical index to study the enzymatic NO· release from L-arginine. This effect is abolished by applying both Nω-nitro-L-arginine methyl ester (NAME) and DL-2-amino-4-phosphonobytyric acid (AP4), and is independent of Ca2+. In contrast, the α-amino-3-hydroxy-5-methylisoxarole-4-propionnate (AMPA)-induced increase in cGMP content is eliminated by the preincubation of CGrC with 4 mM EGTA-chelated Ca2+.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02444662
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