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  • 1
    Electronic Resource
    Electronic Resource
    FIA for on-line monitoring of important lactic acid fermentation variables (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 1127-1134 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An automated system with semi on-line monitoring of glucose, lactic acid, protein, and optical density during lactic acid fermentations, is set up to study the dynamics of lactic acid bacteria. The analyzers for glucose, lactic acid, and protein are based on flow injection analysis (FIA). The system consists of a laboratory fermentor with a continuous withdrawal system and an analysis system where glucose, lactic acid, and protein concentration are measured together with the optical density of the fermentor sample. The system is controlled by a personal computer.The system response is fast, and it yields a large number of reliable and precise analytical data, whoch is of great importance for mathematical model building. Some premliminary results are shown.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260330908
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  • 2
    Electronic Resource
    Electronic Resource
    Compartment model concept used in the design of fermentation with recombinant microorganisms (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 34 (1989), S. 478-486 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Simple models are of great value in order to simulate cell dynamics, and a class of potentially useful models are the so-called compartment models, which are a simple extension of the unstructured approach. Compartment models have earlier been used with great success to describe cell dynamics. In this article the authors present a 4-compartment model that is able to describe a majority of the observations that have been made through fermentations with recombinant microorganisms. The compartment model is especially suited for description of dynamic changes in plasmid copy number, e.g., runaway replication. Simulation of batch fermentation with runaway conditions corresponds very well with experimental results. Finally the model is used in the design of an optimal fermentation strategy in a CSTR.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260340407
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  • 3
    Electronic Resource
    Electronic Resource
    Morphology of Trichoderma reesei QM 9414 in submerged cultures (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 47 (1995), S. 609-615 
    Details
    ISSN: 0006-3592
    Keywords: Trichoderma reesei ; image analysis ; morphology ; modeling ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The microscopic morphology of Trichoderma reesei QM 9414, growing in submerged culture, was studied by image analysis. The morphology was characterized by the total hyphal length, the total number of tips, the number of actively growing tips, and the length of the main hypha. To describe the growth of a single mycelium a simple model is set-up. The main features of the model are: (1) saturation type kinetics for the tip extension of the individual branches within the mycelium; and (2) random branching with a frequency function, which is proportional to the total hyphal length. The model is used to simulate a population of mycelia, where spore germination is described with a log-normal distribution. From the simulation of the population, the average properties of the mycelia, e.g., the average total hyphal length, are calculated, and by fitting the model to experimental data the model parameters are estimated. Finally, the distribution function with respect to the mycelia properties, that is, number of tips and total hyphal length, is calculated, and it corresponds well with the experimental determination of the distribution function. © 1995 John Wiley & Sons Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260470513
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  • 4
    Electronic Resource
    Electronic Resource
    Semi-on-line analysis for fast and precise monitoring of bioreaction processes (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 52 (1996), S. 237-247 
    Details
    ISSN: 0006-3592
    Keywords: bioreactor monitoring ; semi-on-line analysis ; aseptic sampling ; FIA ; SIA ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Monitoring of substrates and products during fermentation processes can be achieved either by on-line, in situ sensors or by semi-on-line analysis consisting of an automatic sampling step followed by an ex situ analysis of the retrieved sample. The potential risk of introducing time delays and signal bias during sampling makes it necessary to distinguish between real-time, on-line, in situ methods and semi-on-line analysis. In addition, semi-on-line analyzers are often mechanically complex - a circumstance which has to be given special attention during their industrial use on a routine basis. This review on semi-on-line analysis will focus both on the dynamics and precision of aseptic sampling devices and on the performance of flow injection analysis (FIA) and sequential injection analysis (SIA), especially with regard to their robustness when used in industry. © 1996 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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