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  • Two-dimensional polyacrylamide gel electrophoresis  (29)
  • Analytical Chemistry and Spectroscopy  (11)
  • 1995-1999  (40)
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  • 1
    Digitale Medien
    Digitale Medien
    Determination of polynuclear aromatic hydrocarbons in environmental samples by Micellar liquid chromatography (1996)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 19 (1996), S. 111-116 
    Details
    ISSN: 0935-6304
    Schlagwort(e): Polynuclear aromatic hydrocarbons ; Liquid chromatography ; Alcohols ; Micelles ; Enviromental samples ; Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: A method for the separation of polycyclic aromatic hydrocarbons (PAHs) by high-performance liquid chromatography using a hybrid micellar mobile phase is described. The detection of PAHs was carried out using the fluorescence method with programmable excitation and emission wavelength. The method is applied to the analysis of several environmental samples (sea water, sediments, limpets, sea worms) and several of these compounds are quantitated at concentration below 70 ng L-1(kg-1) in the original samples.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jhrc.1240190210
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  • 2
    Digitale Medien
    Digitale Medien
    The effect of the central linkage on the mass spectrometric behaviour of extended tetrathiafulvalenes (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 856-861 
    Details
    ISSN: 0951-4198
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Physik
    Notizen: The electron ionization-induced fragmentation pattern of extended tetrathiafulvalenes has been studied by exact mass measurements, metastable ion analysis and collision-induced dissociation mass spectrometry. The influence on the fragmentation of the central bridge linking the dithiole moieties is discussed.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/rcm.1290090928
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  • 3
    Digitale Medien
    Digitale Medien
    Surface-enhanced resonance raman spectroscopy of hypericin and emodin on silver colloids: SERRS and NIR FTSERS study (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biospectroscopy 1 (1995), S. 265-273 
    Details
    ISSN: 1075-4261
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Physik
    Notizen: SERS, SERRS and NIR FTSERS of emodin and hypericin are reported for the first time on aqueous silver colloid. Intense SERRS spectra can be obtained when using excitation lines at 514 nm for emodin and 598 nm for hypericin. FTIR and NIR FTRaman spectra from these molecules and their model compounds, anthrone and bianthrone, were recorded to assist in the assignment of the SERS bands. From the analysis of the SERS spectra a different orientation for each molecule can be deduced. © 1995 John Wiley & Sons, Inc.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bspy.350010405
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  • 4
    Digitale Medien
    Digitale Medien
    Excitation profiles of the surface enhanced raman spectroscopy bands of 1,5-dimethylcytosine on silver colloids (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biospectroscopy 2 (1996), S. 243-248 
    Details
    ISSN: 1075-4261
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Physik
    Notizen: The study of the surface enhanced Raman spectroscopy (SERS) excitation profiles of 1,5-dimethylcytosine on metal colloids allows a more detailed determination of the behavior of the functional groups of this molecule in the adsorption process on the metal supports. Silver has shown its effectiveness and selectivity as a support for this technique from the point of view of its stability and capability to enhance the Raman signal coming from the adsorbate. The results are discussed in terms of the two well-known theories proposed up to now to explain the SERS phenomenon: electromagnetic and chemical (or charge transfer) models. © 1996 John Wiley & Sons, Inc.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Fluorescence and surface-enhanced Raman study of 9-aminoacridine in relation to its aggregation and excimer emission in aqueous solution and on silver surface (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biospectroscopy 4 (1998), S. 327-339 
    Details
    ISSN: 1075-4261
    Schlagwort(e): 9-aminoacridine ; fluorescence ; SERS ; dimerization ; excimer ; Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Physik
    Notizen: Fluorescence spectroscopy and surface-enhanced Raman spectroscopy (SERS) have been applied to study the aggregation and excimer emission of 9-aminoacridine (9AA) and 9-aminoacridine hydrochloride (9AA-HCl) in aqueous solution and on silver colloids. The effect of the drug concentration, pH, and chloride concentration on these processes has been investigated. The excimer emission of 9AA is connected to the dimerization of this drug in solution: the formation of 9AA dimers is greatly favored when the drug is under the amino form at neutral and acidic pH, while at alkaline pH the imino 9AA form tends to form large-sized aggregates which cannot be excited to render excimer emission. 9AA is adsorbed on the silver surface under two different forms: strongly and weakly attached 9AA, each one corresponding to the different drug tautomers: imino and amino. The interaction of 9AA with silver induces a charge transfer from the adsorbate to the metal leading to a remarkable fluorescence quenching, a basicity decrease of the adsorbed drug and a considerable weakening of the dimer-excimer emission. Furthermore, an attribution of the main Raman features appearing in the SERS spectra has been proposed, providing marker bands for the imino and amino 9AA tautomers, and a mechanism for the molecular dimerization is also suggested. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 327-339, 1998
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Surface-enhanced Raman spectroscopy of γ-aminobutyric acid on silver colloid surfaces (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biospectroscopy 3 (1997), S. 449-455 
    Details
    ISSN: 1075-4261
    Schlagwort(e): surface-enhanced Raman spectroscopy ; γ-aminobutyric acid ; Raman spectra ; Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Physik
    Notizen: The surface-enhanced Raman spectroscopy (SERS) of γ-aminobutyric acid (GABA) adsorbed on silver colloids in H2O and D2O were recorded and analyzed. When the concentration is greater than 10-3 M, the adsorbed species is the anionic form of the amino acid that interacts with the surface through both functional groups. According to the vibrational interpretation of the spectra, it is assumed that at concentrations in the order of or less than 10-3 M, GABA undergoes chemical transformations, which increase upon dilution, and spectra are recorded that are the result of the competitive adsorption between the amino acid in its anionic form and the products resulting from the chemical transformations. © 1997 John Wiley & Sons, Inc. Biospectroscopy 3: 449-455, 1997
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    2,3-Di-O-pentyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin as a Chiral Stationary Phase in Capillary Gas Chromatography (1998)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 21 (1998), S. 225-233 
    Details
    ISSN: 0935-6304
    Schlagwort(e): Chiral GC ; chiral stationary phase ; cyclodextrin ; Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: ---2,3-Di-O-pentyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin has been evaluated as an enantioselective stationary phase for capillary gas chromatography. Experimental results show a good enantioselectivity towards compounds with different functional groups (haloalkanes, alcohols, esters, terpenoids, amino acid derivatives, and heterocycles). Column stability improves mixing the chiral phase with polysiloxane SE-54 (1 : 1).
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    L-subshell Coster-Kronig yields for Tb (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    X-Ray Spectrometry 24 (1995), S. 221-225 
    Details
    ISSN: 0049-8246
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Physik
    Notizen: The synchrotron photoionization method was applied to measure L-subshell Coster-Kronig yields for Tb. This method is based on the capability of tuning the energy of the synchrotron photons producing a selective subshell ionization. A foil sample of Tb was irradiated and characteristic spectra were recorded. Data were analysed using a new formalism (based on a matrix representation) to express x-ray fluorescence intensities involving Coster-Kronig transitions. The results obtained were f12 = 0.201 ± 0.014, f13 = 0.281 ± 0.027 and f23 = 0.097 ± 0.016. These data represent valuable information for spectroscopists, considering the lack of data for L-shell parameters.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/xrs.1300240502
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  • 9
    Digitale Medien
    Digitale Medien
    Phenotyping of apolipoprotein E using immobilized pH gradient gels for one-dimensional and two-dimensional separations (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1184-1186 
    Details
    ISSN: 0173-0835
    Schlagwort(e): Apolipoprotein E ; Immobilized pH gradient ; Phenotyping ; Immunoblotting ; Two-dimensional polyacrylamide gel electrophoresis ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Apolipoprotein E (apo E) is a normal component of several classes of plasma lipoproteins. Apo E phenotypes are closely related to total cholesterol, low density lipoprotein (LDL)-cholesterol and apo B concentration. The apo E 2/2 phenotype is related to the type III hyperlipoproteinemia due to the defective binding of apo E-2 to the hepatic receptors. The apo E 4/4 phenotype has been reported to be present in most elderly people suffering from the Alzheimer disease, and is associated with increased risk of coronary heart disease and Creutzfeld-Jakob disease. Therefore, apo E phenotyping is essential. The method described here uses a precast immobilized pH gradient, avoids time-consuming separation of lipoproteins from plasma, needs no pretreatment with neuraminidase and involves highly sensitive enhanced chemiluminescence for visualization. Therefore it has many advantages over previously published methods.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/elps.11501601196
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  • 10
    Digitale Medien
    Digitale Medien
    The yeast SWISS-2DPAGE database (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 556-565 
    Details
    ISSN: 0173-0835
    Schlagwort(e): Yeast ; SWISS-2DPAGE ; Two-dimensional polyacrylamide gel electrophoresis ; Protein database ; Protein mapping ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: The systematic sequencing of the yeast genome will soon be completed. A new challenge has been launched by the EUROFAN (European Functional Analysis) project whose goal is to elucidate the physiological and biochemical function of newly discovered open reading frames (ORF) from yeast. One of the approaches is to use protein-based technologies such as two-dimensional gel eletrophoresis and protein identification in order to establish a yeast reference map. Modified protein patterns can be compared to the reference map which hopefully will help identify changes related, for example, to growth processes or developmental events. This paper describes the yeast SWISS-2DPAGE database in which charge separation was obtained using immobilized pH gradient (IPG). Proteins identified by gel comparison, amino acid composition analysis and/or microsequencing are recorded and described in an accessible uniform format. We have identified more than one hundred polypeptides, several of which were newly mapped. In addition, the yeast SWISS-2DPAGE database can be freely accessed through the World Wide Web (WWW) network on the ExPASy molecular biology server.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/elps.1150170326
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