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  • Wiley-Blackwell  (32)
  • American Geophysical Union (AGU)
  • Hindawi
  • 1995-1999  (19)
  • 1985-1989  (14)
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  • 1
    Electronic Resource
    Electronic Resource
    Single-cell protein from methanol with Enterobacter aerogenes (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 29 (1987), S. 355-357 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An identifiedEnterobacter aerogenesutilizing methanol as a sole carbon source was studied for the optimization of biomass production and the reduction of its nucleic acid content. Results indicated that the highest yield and conversion were obtained at 0.5% methanol. The addition of seawater as a source of trace elements has an adverse effect. However, the addition of urea as source of nitrogen enhanced the growth of E. aerogenes. Heat shock at 60°C for 1 min followed by incubation at 50°C for 2 h caused 72.6% reduction in the nucleic acid.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260290310
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular and crystal structures of three monothiated analogues of the terminally blocked ala-aib-ala sequence of peptaibol antibiotics (1988)
    New York : Wiley-Blackwell
    Biopolymers 27 (1988), S. 747-761 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The molecular and crystal structures of three monothiated analogues of the blocked L-Ala-Aib-L-Ala sequence of peptaibol antibiotics, t-Boc-L-Ala-Aib-ψ(CSNH)-L-Ala-OMe, Ac-L-Ala-Aib-ψ(CSNH)-L-Ala-OMe, and Ac-ψ(CSNH)-L-Ala-Aib-L-Ala-OMe, determined by x-ray diffraction analyses, are reported. In all cases the peptide chain is folded with ϕ,ψ angles close to or slightly distorted from those expected for a type II β-bend conformation. However, the 4 → 1 H-bond distance falls within the accepted limits only for Ac-L-Ala-Aib-ψ(CSNH)-L-Ala-OMe. The structures are compared with those already published for their two oxygenated analogues.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360270504
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  • 3
    Electronic Resource
    Electronic Resource
    Polymerization and conformational studies of poly(trans-3-ethyl-D and L-proline) (1987)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 25 (1987), S. 2941-2952 
    Details
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Poly(L-trans-3-ethylproline), L-PT3EP, and poly(D-trans-3-ethylproline), D-PT3EP, were prepared by ring opening polymerization of the corresponding N-carboxyanhydrides (NCA) using triethylamine as an initiator. Using circular dichroism spectroscopy, it was shown that the incorporation of an ethyl group at the 3 position of the pyrrolidine ring caused a noticeable change in the conformational behavior of the polymer in solution. The ethyl group limited to some extent rotation of the polymer chain around the C——CO bond and prevented the mutarotation between the two forms found in poly-L-proline polymers.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1987.080251102
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  • 4
    Electronic Resource
    Electronic Resource
    Synthesis, separation, and resolution of cis- and trans-3-ethylproline (1987)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 25 (1987), S. 3437-3458 
    Details
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The synthesis, separation, and optical resolution of cis- and trans-3-ethylproline are described. Two different approaches were employed: (1) The Michael addition reaction of 2-pentenal with diethyl-N-carbobenzyloxyaminomalonate gave the intermediate 3-ethyl-5-hydroxy-N-benzyloxypyrrolidine. Hydrogenolysis of this intermediate followed by acid hydrolysis gave a mixture of cis- and trans-3-ethylproline. Separation of the isomers was accomplished by selective saponification of N-(p-toluenesulfonyl)-cis- and trans-3-ethylproline methyl esters using 0.25N methanolic sodium hydroxide. (2) The Michael condensation of diethyl acetamidomalonate with 2-pentenoic acid ethyl ether produced the intermediate 5,5-bis(ethoxycarbonyl)-4-ethylpyrrolidine. Partial saponification followed by decarboxylation afforded a mixture of cis- and trans-isomers of ethyl-3-ethylpyroglutamate. The diastereoisomers were separated using low temperature fractional crystallization. Reduction of these isomers and tosylation in situ afforded the corresponding N-(p-toluenesulfonyl)-cis- and trans-3-ethylprolinols. Chromic acid oxidation gave N-(p-toluenesulfonyl)-cis- and trans-3-ethylproline. Reaction of these tosylates with 30% hydrogen bromide in acetic acid gave cis- and trans-3-ethylproline. Both optically active isomers of D(+)-and L(-)-trans-3-ethylproline were successfully resolved using (+)-dibenzoyl-D-tartaric acid and (-)-dibenzoyl-L-tartaric acid as resolving agents. The absolute configurations of the optically active isomers were determined by circular dichroism spectroscopy.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1987.080251224
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  • 5
    Electronic Resource
    Electronic Resource
    An amphiphilic graft copolymer as a surface-modifying additive for poly(methyl methacrylate) (1995)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 33 (1995), S. 1257-1265 
    Details
    ISSN: 0887-624X
    Keywords: poly(methyl methacrylate) ; amphiphlic additive ; graft copolymer ; surface properites ; surface modification ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An amphiphilic graft copolymer was prepared by transesterification of poly(2-ethylhexyl acrylate-co-methyl methacrylate) with poly(ethylene glycol) monomethyl ether (MPEG2000). The grafting reaction was performed in melt at 155°C. The purified graft copolymer was blended into poly(methyl methacrylate) in concentrations of 1.5-30 wt %, either by mixing in chloroform solution or by melt mixing by means of a twin-screw extruder or a Brabender blender. Films of the blends were prepared by solution casting onto glass plates or by hot pressing between polished Al plates. At concentrations up to 20% of the graft copolymer homogeneous blends were obtained. At higher concentrations the blends were heterogeneous, and side-chain crystallinity was detectable by DSC analysis. The surface properties of the films were studied by measurements of water contact angles. The surface accumulation of the graft copolymer was demonstrated as a large increase in the wetting angle hysteresis, and found to depend on the procedure for film preparation as well as the casting substrate. © 1995 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1995.080330808
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  • 6
    Electronic Resource
    Electronic Resource
    Correlation between the distribution of smooth muscle or non muscle myosins and α-smooth muscle actin in normal and pathological soft tissues (1988)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 11 (1988), S. 260-274 
    Details
    ISSN: 0886-1544
    Keywords: cytoskeleton ; atheromatosis ; wound healing ; fibromatosis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The distribution of smooth muscle (SM) and non muscle myosins was compared with that of α-SM actin in various normal and pathological tissues and in cultured cells by means of indirect immunofluorescence using a monoclonal antibody specific for α-SM actin [anti-αsm-1, Skalli et al., 1986b] and two polyclonal antibodies raised against bovine aortic myosin (ABAM) and human platelet myosin (AHPM), respectively.In normal tissues ABAM stained vascular and parenchymal smooth muscle cells (SMC), myoepithelial cells and myoid cells of the testis in a pattern similar to that reported by other authors with antisera raised against non vascular SM myosin. Cells stained with ABAM were always positive for anti-αsm-1. In human and experimental atheromatous plaques, most cells were positive for AHPM; a variable proportion was also stained for ABAM plus anti-αsm-1. Myofibroblasts from rat granulation tissue, Dupuytren's nodule and stroma from breast carcinoma were constantly positive for AHPM and negative for ABAM; however, myofibroblasts from Dupuytren's nodule and breast carcinoma were anti-αsm-1 positive. Early primary cultures of rat aortic SMC were positive for ABAM and anti-αsm-1 and became negative for ABAM and positive for AHPM after a few days in culture. They remained positive for AHPM and anti-αsm-1 after passages; the staining of AHPM and anti-αsm-1 appeared to be colocalized along the same stress fibers.These results may be relevant for the understanding of SMC function and adaptation, and show that in non malignant SMC proliferation, α-SM actin represents a more general marker of SM origin than SM myosin.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970110405
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  • 7
    Electronic Resource
    Electronic Resource
    Topotactic solid-state polymerization of 3-aminocrotonamide by radiation (1996)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 34 (1996), S. 1087-1093 
    Details
    ISSN: 0887-624X
    Keywords: topotactic polymerization ; 3-aminocrotonamide ; radiation polymerization ; crystal structure ; condensation polymerization ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Radiation-induced solid-state polymerization of 3-aminocrotonamide (3-amino-2-butenamide) was carried out at room temperature, in open air atmosphere and under vacuum condition. The polymer obtained was white powder, soluble in methanol, but insoluble in water. The nature of polymers were investigated by IR, UV, x-ray, DP-MS, and elemental analysis to elucidate the mechanism of the polymerization. The polymer was crystalline with melting point in the range of 245-255°C. The cell parameters and space group of monomer and polymers were determined from powder x-ray diffraction patterns. The similarity of crystal structures of monomer and polymer indicated a topotactic polymerization. It was shown by spectroscopic investigations and elemental analyses that the polymerization proceeds by condensation reaction with evolution of one mole ammonia per two combined moles of monomer through a free radical mechanism. © 1996 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    An inverse solution procedure for material parameters identification in large plastic deformations (1996)
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 12 (1996), S. 161-173 
    Details
    ISSN: 1069-8299
    Keywords: inverse identification ; non-linear behaviour ; material parameters ; sensitivity analysis ; finite elements ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The identification of materials rheological behaviour in the non-linear range is based on experimental tests. When using direct identification methods, one faces the problem of the interpretation of the experimental tests, which requires the assumption of deformation homogeneity and therefore the use of approximation methods. Since this assumption is often not satisfied in the case of non-linear behaviour, material parameters are not assessed precisely. In the paper, an inverse identification method is proposed to avoid the problems raised by interpretation of the experimental tests and to determine material parameters more accurately. The algorithm developed consists of both an optimization method and a finite element method. This method is applied to the inverse identification of viscoplastic parameters of an aluminium alloy, with an investigation on the effect of the initial guess and errors in experimental data on the identified values.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic evidence that the steroid-regulated trafficking of cell surface glycoproteins in rat hepatoma cells is mediated by glucocorticoid-inducible cellular components (1987)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 35 (1987), S. 271-284 
    Details
    ISSN: 0730-2312
    Keywords: glycoprotein trafficking variant ; mouse mammary tumor virus ; heterokaryons ; glucocorticoid receptor deficient variant ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The biological control of posttranslational maturation and compartmentalization reactions that operate upon proteins during transport to their final cellular desti- nations is crucial for normal cellular function. Using the expression of mouse mammary tumor virus (MMTV) glycoproteins as sensitive probes in the viral- infected rat hepatoma cell line M1.54, we have discovered and documented a novel glucocorticoid-regulated trafficking pathway that controls the cell surface localization of MMTV glycoproteins. One complement-selected derivative of M1.54 cells, CR4, failed to compartmentalize cell surface MMTV glycoproteins in the presence of dexamethasone. To test genetically if this glycoprotein trafficking pathway is mediated by cellular or viral gene products, CR4 cells were fused with uninfected Fu5 rat hepatoma cells. Indirect immunofluorescence of CR4 × Fu5 heterokaryons revealed that Fu5 complemented the defect in CR4 only after exposure to 1 μM dexamethasone. The glucocorticoid inhibition of Fu5 proliferation was exploited to recover the receptor-deficient uninfected derivative EDR3 that expressed a 100-fold lower level of [3H]dexamethasone binding activity. Analysis of CR4 × EDR3 cell fusions by indirect immunofluorescence revealed that EDR3 cells complemented CR4 in a dexamethasone-dependent manner, suggesting that EDR3 supplied a functional trafficking component while CR4 provided a functional glucocorticoid receptor to the heterokaryons. Taken together, our results demonstrate that cellular-encoded glucocorticoid-inducible components mediate the regulated trafficking of cell surface MMTV glycoproteins.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240350402
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  • 10
    Electronic Resource
    Electronic Resource
    Prothrombin cleavage by human vascular smooth muscle cells: A potential alternative pathway to the coagulation cascade (1995)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 59 (1995), S. 514-528 
    Details
    ISSN: 0730-2312
    Keywords: prothrombin ; prethrombin 2 ; fragment 1.2 ; α-thrombin ; prothrombin activation ; serine proteinase ; human vascular smooth muscle cells ; mitogenic activity ; enzymatic activity ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Thrombin is a potent mitogen for human vascular smooth muscle cells (HVSMC) and its enzymatic activity is required for this function. The present study demonstrates that prothrombin is also mitogenic for HVSMC due to the generation of enzymatically active thrombin which occurs upon incubation of prothrombin with the cells. Analysis by SDS-PAGE, immunoblotting, and amino acid sequencing revealed that prothrombin incubated with HVSMC undergoes limited proteolysis. Prethrombin 1 was formed through cleavage at R155-S156. Cleavage at R271-T272 generated fragment 1.2 and prethrombin 2 whilst cleavage at R284-T285 yielded truncated prothrombin 2 (prethrombin 2′). However, cleavage at R320-I321 which, during prothrombin activation produces two-chain α-thrombin, was not detectable. Studies on HVSMC-conditioned medium revealed that a similar pattern of prothrombin cleavage occurred by a cell-secreted factor(s). Amidolytic activity analysis indicated that 1-3% catalytically active thrombin-like activity was generated upon incubation of prothrombin with HVSMC-conditioned medium. By treating conditioned medium with various classes of proteinase inhibitors or hirudin, it was determined that prothrombin is cleaved by a cell-derived serine proteinase-like factor(s) at R271-S272 and by α-thrombin at R155-S156 and R284-T285. Antibodies neutralising the activity of either urokinase, tissue plasminogen activator, or factor Xa failed to alter the prothrombin cleaving activity of conditioned medium. This activity which may catalyse an alternative pathway for the generation of thrombin, was eluted from a gel filtration column as a single peak with apparent molecular mass of 30-40 kDa. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240590411
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