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  • Animals  (5)
  • Polymer and Materials Science  (5)
  • *Genes, Regulator
  • 1995-1999  (6)
  • 1985-1989  (5)
  • 1940-1944
  • 1
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electroanalysis 1 (1989), S. 1-11 
    ISSN: 1040-0397
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A review of the various simultaneous ESR-electrochemical techniques that have been developed is presented. Special advantages and disadvantages of the stationary and flow-through cell configurations are described. A critical evaluation of the in situ ESR-electrochemical cells developed to date has also been made. A selected survey of applications is presented to illustrate the wide range of chemical information on redox reactions which can be obtained by the simultaneous ESR-electrochemical method.
    Additional Material: 4 Ill.
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  • 2
    ISSN: 1040-0397
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Gold and platinum ultrathin ring microelectrodes (0.1-0.5 mm thick, 1.5-4 mm diameter) were made by sputtering, from fired metalloorganic paint, and from foil for use as sensors in a thick-layer wall-jet flow cell. An end-on array of three 0.1 μm × 0.5 mm gold band electrodes were mounted parallel to the flow direction in a thin-layer channel cell. These ultrathin sensors, along with a jet-centered carbon microdisk, were evaluated in the amperometric flow-injection mode for temporal stability, calibration sensitivity, detectivity, background signal, and flow rate dependence of the analytical signal using ferrocene samples in acetonitrile containing 10-4-10-2 M TEAP. The detectivity of gold paint ring electrodes made on borosilicate glass was 3-6 nM, an order of magnitude lower than any of the other electrodes tested. Analytical signals from gold paint and foil rings and the carbon microdisk had the best temporal stability. The current for the ultrathin band array in a channel cell was flow rate-independent, and the exponential dependence of cell current on flow rate was 0.11-0.14 for ultrathin rings in the thick-layer wall-jet mode.
    Additional Material: 9 Ill.
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  • 3
    ISSN: 1040-0397
    Keywords: Antimony ; Stripping analysis ; Zinc plant electrolyte ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Antimony, in both its trivalent and pentavalent states may be detrimental to the electrochemical deposition of zinc. Ideally, therefore antimony should be monitored in both oxidation states throughout an electrolytic zinc plant. At hydrochloric acid concentrations ( 〉 4 M) both antimony(III) and (V) present in zinc plant electrolyte can be reduced at a hanging mercury drop electrode (HMDE) to form an antimony amalgam, Sb(Hg). Consequently a very sensitive analytical signal is obtained for the determination of total antimony by differential pulse anodic stripping voltammetry (DPASV) using a 1:1 mixture of plant electrolyte and concentrated hydrochloric acid. In contrast, at low acid concentrations (0.1 M) predominantly only antimony(III) can be reduced and therefore determined in zinc plant electrolyte by DPASV at a HMDE by use of a 1:1 mixture of plant electrolyte and 0.2 M hydrochloric acid. The antimony(V) concentration is calculated by the method of subtraction of antimony(III) from total antimony. Consequently, simple addition of high and low concentrations of hydrochloric acid to zinc plant electrolyte can be used to provide a suitable electrolyte for both off-line and on-line methods for determining the concentration of total antimony, antimony(III) and antimony(V). The method described in this article has been applied to a wide range of zinc electrolyte samples and an on-stream analyzer technique has been successfully used for several years at the Pasminco Metals-BHAS electrolytic zinc plant in Port Pirrie, Australia.
    Additional Material: 10 Ill.
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  • 4
    ISSN: 1040-0397
    Keywords: Electrochemical quartz crystal microbalance ; Tetrathiafulvalene ; Mechanism ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Simultaneous voltammetric or double potential step and quartz crystal microbalance experiments combined with the results obtained from optical and surface analysis measurements have allowed new mechanistic aspects of the electrochemical oxidation of microcrystalline particles of tetrathiafulvalene (TTF) attached to a gold substrate, which is placed in aqueous electrolyte media, to be unravelled. The use of the microcrystalline form of the solid rather than thin films makes it possible to obtain short time domain data which enables features for the reduction and the oxidation process consistent with a nucleation process at the solid electrode-aqueous electrolyte interface to be detected. Electrochemical quartz crystal microbalance (EQCM) and electron microprobe analysis experiments confirm that the overall process in the oxidation of microcrystals of TTF in aqueous KBr, KI, KClO4, NaBr and CsBr electrolyte media involves the uptake of the appropriate nonsolvated anion (X-) in order to maintain charge neutrality. Reduction of the oxidized TTF leads to the expulsion of the anion. The rates of uptake and expulsion are both very rapid for the bromide ion, but involve slower reaction steps for iodide and perchlorate anions. The oxidation of TTF particles attached to a gold electrode can be described by the equation \documentclass{article}\pagestyle{empty}\begin{document}$ \mathop {\rm{TTF}}_{{\rm{(solid)}}} + n{\rm{X}}_{{\rm{(solution)}}}^ - {\rm{TTFX}}_{n({\rm{solid}})} + ne^ - $\end{document} The number of electrons transferred to the solid, n, may theoretically vary over a wide range, but in the case of X- =Br- is found to be fixed with only one product formed (n =0.7). The peak potential and shape of voltammograms have been studied as a function of scan rate, electrolyte concentration and the nature of the anion and cation. A Nernstian change in peak potential is observed when the electrolyte anion concentration is varied. The wave shape, but not the charge, also varies with electrolyte anion and concentration. In contrast, no dependence on the electrolyte cation is found. All the data imply that incorporation of the anion into the solid provides the charge neutralization mechanism. This study confirms that use of microcrystalline forms of a solid enables a wide range of time domains (and techniques) to be applied to electrochemical studies of solids.
    Additional Material: 14 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electroanalysis 9 (1997), S. 681-684 
    ISSN: 1040-0397
    Keywords: Anodic stripping voltammetry ; Ion-exchange column ; Copper plant electrolyte ; Total antimony ; Antimony(III) and antimony(V) ; Hanging mercury drop electrode ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The concentration of antimony in copper plant electrolyte needs to be known at the ppm level. Spectroscopic techniques for trace metal determination in this electrolyte, such as atomic absorption and inductively coupled plasma (ICP) spectrometry only enable total antimony to be determined, whereas ideally the concentration of both the antimony(III) and antimony(V) oxidation states needs to be known. For the determination of antimony(III) and antimony(V) by differential pulse anodic stripping voltammetry (DPASV), the similar stripping peak potentials of -0.37 V(vs. Ag/AgCl) for copper and -0.27 V(vs. Ag/AgCl) for antimony in 5 M HCl mean that concentrations of copper greater than 6 times that of antimony cause difficulties in resolving the antimony and copper stripping peaks. In this article, a simple procedure is reported for the determination of antimony(III) and (V) in copper plant electrolyte after separation of antimony from copper by passing an ammoniacal solution of plant electrolyte through a column of Chelex-100 ion-exchange resin. Most of the copper is retained on the column so that the determination of antimony(III) and (V) by DPASV is possible after addition of hydrochloric acid to the eluent. Total antimony is determined in 5 M HCl and antimony(III) in 0.1 M HCl. Total antimony concentrations correlate well with data obtained by ICP and recoveries of antimony in both oxidation states are 〉 96%.
    Additional Material: 5 Ill.
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  • 6
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1997-02-21
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bond, E -- Austin, M J -- New York, N.Y. -- Science. 1997 Feb 21;275(5303):1051-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9054002" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Candida albicans/*genetics ; Foundations ; *Genome ; Genome, Fungal ; Genome, Protozoan ; Great Britain ; International Cooperation ; National Institutes of Health (U.S.)/economics ; Plasmodium falciparum/*genetics ; *Research Support as Topic ; *Sequence Analysis, DNA ; United States
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
    Publication Date: 1996-09-20
    Description: Members of a previously unidentified family of potassium channel subunits were cloned from rat and human brain. The messenger RNAs encoding these subunits were widely expressed in brain with distinct yet overlapping patterns, as well as in several peripheral tissues. Expression of the messenger RNAs in Xenopus oocytes resulted in calcium-activated, voltage-independent potassium channels. The channels that formed from the various subunits displayed differential sensitivity to apamin and tubocurare. The distribution, function, and pharmacology of these channels are consistent with the SK class of small-conductance, calcium-activated potassium channels, which contribute to the afterhyperpolarization in central neurons and other cell types.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kohler, M -- Hirschberg, B -- Bond, C T -- Kinzie, J M -- Marrion, N V -- Maylie, J -- Adelman, J P -- New York, N.Y. -- Science. 1996 Sep 20;273(5282):1709-14.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Vollum Institute, L-474, Oregon Health Sciences University, 3181 Southwest Sam Jackson Road, Portland, OR 97201, USA. J. Maylie, Department of Obstetrics and Gyne.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8781233" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Antisense Elements (Genetics) ; Apamin/pharmacology ; *Brain Chemistry ; Calcium/*metabolism/pharmacology ; Cloning, Molecular ; Electric Conductivity ; Female ; Humans ; Membrane Potentials ; Molecular Sequence Data ; Neurons/*physiology ; Oocytes ; Patch-Clamp Techniques ; Potassium/metabolism ; Potassium Channel Blockers ; Potassium Channels/analysis/chemistry/*physiology ; *Potassium Channels, Calcium-Activated ; RNA, Messenger/analysis/genetics ; Rats ; Rats, Sprague-Dawley ; Small-Conductance Calcium-Activated Potassium Channels ; Xenopus
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
    Publication Date: 1995-06-02
    Description: Transgenic mice were created with cardiac-specific overexpression of the beta-adrenergic receptor kinase-1 (beta ARK1) or a beta ARK inhibitor. Animals overexpressing beta ARK1 demonstrated attenuation of isoproterenol-stimulated left ventricular contractility in vivo, dampening of myocardial adenylyl cyclase activity, and reduced functional coupling of beta-adrenergic receptors. Conversely, mice expressing the beta ARK inhibitor displayed enhanced cardiac contractility in vivo with or without isoproterenol. These animals demonstrate the important role of beta ARK in modulating in vivo myocardial function. Because increased amounts of beta ARK1 and diminished cardiac beta-adrenergic responsiveness characterize heart failure, these animals may provide experimental models to study the role of beta ARK in heart disease.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Koch, W J -- Rockman, H A -- Samama, P -- Hamilton, R A -- Bond, R A -- Milano, C A -- Lefkowitz, R J -- 5F32-CA09350/CA/NCI NIH HHS/ -- HL-16037/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1995 Jun 2;268(5215):1350-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7761854" target="_blank"〉PubMed〈/a〉
    Keywords: Adenylyl Cyclases/metabolism ; Animals ; Blood Pressure ; Cyclic AMP-Dependent Protein Kinases/antagonists & ; inhibitors/genetics/*metabolism ; GTP-Binding Proteins/metabolism ; Heart/*physiology ; Isoproterenol/pharmacology ; Mice ; Mice, Transgenic ; *Myocardial Contraction/drug effects ; Myocardium/*enzymology ; Phenotype ; Receptors, Adrenergic, beta/*metabolism ; Sarcolemma/enzymology ; Ventricular Pressure ; beta-Adrenergic Receptor Kinases
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
    Publication Date: 1986-02-21
    Description: Partial amino acid sequence analysis of a purified lymphocyte homing receptor demonstrates the presence of two amino termini, one of which corresponds precisely to the amino terminus of ubiquitin. This observation extends the province of this conserved polypeptide to the cell surface and leads to a proposed model of the receptor complex as a core polypeptide modified by glycosylation and ubiquitination. Independent antibodies to ubiquitin serve to identify additional cell surface species, an indication that ubiquitination of cell surface proteins may be more general. It is proposed that functional binding of lymphocytes to lymph node high endothelial venules might involve the ubiquitinated region of the receptor; if true, cell surface ubiquitin could play a more general role in cell-cell interaction and adhesion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Siegelman, M -- Bond, M W -- Gallatin, W M -- St John, T -- Smith, H T -- Fried, V A -- Weissman, I L -- AI 19512/AI/NIAID NIH HHS/ -- CA 09151/CA/NCI NIH HHS/ -- GM 31461/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1986 Feb 21;231(4740):823-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3003913" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Antibodies, Monoclonal ; Cell Movement ; Endothelium/metabolism ; Glycoproteins/metabolism/*physiology ; Glycoside Hydrolases/metabolism ; High Mobility Group Proteins/*metabolism ; Lymphocytes/*physiology ; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase ; Membrane Proteins/metabolism/*physiology ; Mice ; Molecular Weight ; Protein Processing, Post-Translational ; Receptors, Cell Surface/metabolism/*physiology ; Ubiquitins/immunology/*metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 10
    Publication Date: 1987-03-20
    Description: The thymidine kinase (tk) promoter of herpes simplex virus includes an octanucleotide sequence motif (ATTTGCAT) that is also an essential component of immunoglobulin kappa gene promoters. In the absence of an enhancer, tk promoter derivatives that contain this element support a higher rate of transcription than those that lack it. The action of the kappa enhancer augments that of the octanucleotide in B lymphoid cells; when both elements are present, tk promoter activity is increased by more than an order of magnitude. In contrast, the presence of the octanucleotide in this promoter markedly reduces its response to a nonimmunoglobulin enhancer. These results suggest that the octanucleotide may mediate a selective interaction among promoters and enhancers.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Parslow, T G -- Jones, S D -- Bond, B -- Yamamoto, K R -- AI22536/AI/NIAID NIH HHS/ -- CA20535/CA/NCI NIH HHS/ -- CM07810/CM/NCI NIH HHS/ -- New York, N.Y. -- Science. 1987 Mar 20;235(4795):1498-501.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3029871" target="_blank"〉PubMed〈/a〉
    Keywords: DNA, Viral/genetics ; *Enhancer Elements, Genetic ; *Gene Expression Regulation ; *Genes, Regulator ; Immunoglobulin kappa-Chains/*genetics ; Lymphocytes/physiology ; Moloney murine sarcoma virus/genetics ; *Promoter Regions, Genetic ; Simplexvirus/genetics ; Thymidine Kinase/genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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