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  • Heterologous expression  (3)
  • gastrointestinal transit  (3)
  • Springer  (6)
  • 1995-1999  (3)
  • 1985-1989  (3)
  • 1955-1959
Collection
Keywords
Publisher
  • Springer  (6)
Years
  • 1995-1999  (3)
  • 1985-1989  (3)
  • 1955-1959
  • 1990-1994  (2)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Characterization of theugatA gene ofUstilago maydis, isolated by homology to thegatA gene ofAspergillus nidulans (1996)
    Springer
    Current genetics 29 (1996), S. 360-369 
    Details
    ISSN: 1432-0983
    Keywords: Ustilago maydis ; GABA aminotransferase ; Heterologous expression ; Sequence conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gene encoding a putative GABA aminotransferase (ugatA) was isolated from the basidiomyceteUstilago maydis via heterologous hybridization to the GABA aminotransferase gene (gatA) ofAspergillus nidulans. The derived amino-acid sequence ofugatA shows strong identity throughout the protein to the GABA aminotransferase enzymes fromA. nidulans andSaccharomyces cerevisiae. Northern analysis inU. maydis indicated that theugatA transcript is inducible by the ω-amino acids GABA and β-alanine, and is not subject to nitrogen catabolite repression. With the use ofugatA promoter-lacZ fusion constructs, it was demonstrated that the removal of sequences located approximately 250 by 5′ to the translational start site ofugatA (including multiple copies of a 7-bp direct repeat) resulted in the loss of induction by ω-amino acids. While theugatA gene under the control of theA. nidulans gatA promoter was able to fully complement agatA − phenotype inA. nidulans, the full-lengthugatA gene was not, suggesting a lack of expression from theU. maydis promoter inA. nidulans. AU. maydis strain with a gene disruption at theugatA locus showed decreased growth on β-alanine as a sole nitrogen source, but was able to grow on GABA as a sole nitrogen source, indicating an alternative pathway for the utilization of GABA inU. maydis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02208617
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of the ugatA gene of Ustilago maydis, isolated by homology to the gatA gene of Aspergillus nidulans (1996)
    Springer
    Current genetics 29 (1996), S. 360-369 
    Details
    ISSN: 1432-0983
    Keywords: Key words Ustilago maydis ; GABA aminotransferase ; Heterologous expression ; Sequence conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A gene encoding a putative GABA aminotransferase (ugatA) was isolated from the basidiomycete Ustilago maydis via heterologous hybridization to the GABA aminotransferase gene (gatA) of Aspergillus nidulans . The derived amino-acid sequence of ugatA shows strong identity throughout the protein to the GABA aminotransferase enzymes from A. nidulans and Saccharomyces cerevisiae. Northern analysis in U. maydis indicated that the ugatA transcript is inducible by the ω-amino acids GABA and β-alanine, and is not subject to nitrogen catabolite repression. With the use of ugatA promoter-lacZ fusion constructs, it was demonstrated that the removal of sequences located approximately 250 bp 5′ to the translational start site of ugatA (including multiple copies of a 7-bp direct repeat) resulted in the loss of induction by ω-amino acids. While the ugatA gene under the control of the A. nidulans gatA promoter was able to fully complement a gatA - phenotype in A. nidulans, the full-length ugatA gene was not, suggesting a lack of expression from the U. maydis promoter in A. nidulans. A U. maydis strain with a gene disruption at the ugatA locus showed decreased growth on β-alanine as a sole nitrogen source, but was able to grow on GABA as a sole nitrogen source, indicating an alternative pathway for the utilization of GABA in U. maydis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050057
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  • 3
    Electronic Resource
    Electronic Resource
    Heterologous expression and regulation of the lysA genes of Pseudomonas aeruginosa and Escherichia coli (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 430-434 
    Details
    ISSN: 1617-4623
    Keywords: Pseudomonas ; lysA ; Heterologous expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Pseudomonas aeruginosa lysA gene encoding diaminopimelate decarboxylase (DAP-decarboxylase) was cloned into a broad host range vector. This gene complemented a lys mutation at the lys-12 locus of P. aeruginosa and a lysA defect in Escherichia coli. The P. aeruginosa DAP-decarboxylase was synthesized constitutively in P. aeruginosa as well as in E. coli, where the Pseudomonas lysA gene was poorly expressed. By contrast, the E. coli lysA gene was expressed well in P. aeruginosa and subject to lysine regulation when the E. coli LysR activator protein was provided. This indicates that the mechanism of transcriptional activation for the E. coli lysA gene is effective in the heterologous host.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422067
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  • 4
    Electronic Resource
    Electronic Resource
    The effect of density on the gastric emptying of single- and multiple-unit dosage forms (1986)
    Springer
    Pharmaceutical research 3 (1986), S. 208-213 
    Details
    ISSN: 1573-904X
    Keywords: gastrointestinal transit ; pellet density ; floating formulations, gastric emptying
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The gastric emptying of pellets and single units of different densities has been followed in healthy subjects using the technique of gamma scintigraphy. The gastric emptying of the light pellets was affected by their buoyancy in the upper part of the stomach. However, the mean gastric emptying rates of pellets and single units were not significantly affected by density. Floating or buoyant delivery systems may have little advantage over conventional systems. The presence of food in the stomach was found to be the major factor in determining the gastric emptying of single units.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1016334629169
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  • 5
    Electronic Resource
    Electronic Resource
    Correlation of Phenylpropanolamine Bioavailability with Gastrointestinal Transit by Scintigraphic Monitoring of 111In-Labeled Hydroxypropylmethylcellulose Matrices (1989)
    Springer
    Pharmaceutical research 6 (1989), S. 274-278 
    Details
    ISSN: 1573-904X
    Keywords: bioavailability ; scintigraphy ; gastrointestinal transit ; controlled release ; phenylpropanolamine ; hydroxypropylmethylcellulose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Two controlled-release hydroxypropylmethylcellulose (HPMC) matrix formulations, a single-unit and a multiple-unit system, have been evaluated in human volunteers. Both formulations contained the sympathomimetic drug phenylpropanolamine hydrochloride and each was radiolabeled with 111Inbound Amberlite IR 120 ion-exchange resin. The formulations were administered to each of six healthy male volunteers and gastrointestinal (GI) transit was monitored using a gamma camera. Serum samples were taken at set time intervals and assayed for phenylpropanolamine content, thus allowing blood drug levels to be correlated with the position of the dosage form in the GI tract. The multiple-unit system emptied from the stomach gradually over a period of about 180 min, when administered after a light breakfast, whereas the single-unit dosage forms had extremely variable gastric emptying times (range, 60 to 〉570 min). However, both formulations provided prolonged phenylpropanolamine blood levels. The differences in the blood profiles obtained with the two formulations were attributed to variations in their in vitro release rates and not to any differences in their GI transit times.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1015986121822
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  • 6
    Electronic Resource
    Electronic Resource
    The Effect of Oleic Acid on the Human Ileal Brake and Its Implications for Small Intestinal Transit of Tablet Formulations (1999)
    Springer
    Pharmaceutical research 16 (1999), S. 92-96 
    Details
    ISSN: 1573-904X
    Keywords: ileal brake ; oleic acid ; tablets ; gastrointestinal transit ; scintigraphy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. A human volunteer study was carried out to investigate whether activation of the ileal brake mechanism affects the transit of tablets through the small intestine. Methods. Oleic acid, which has previously been shown to activate the brake, was delivered to the small intestine in a modified release capsule at doses of 300 mg, 600 mg and 1200 mg. The effect of the oleic acid was determined by measuring the transit of two sets of radiolabelled tablets by gamma scintigraphy. One set of tablets was dosed with the capsule and the other one hour later. Results. The results show that in the majority of the volunteers small intestinal residence time was greater with the oleic acid than control. The effect was most pronounced in the tablets given concomitantly with the capsule and with the higher doses of oleic acid. Conclusions. The ileal brake, activated by oleic acid, can slow the transit of tablets through the small intestine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018827030210
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