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  • 1
    Electronic Resource
    Electronic Resource
    DNA amplification fingerprinting analysis of bermudagrass (Cynodon): genetic relationships between species and interspecific crosses (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 228-235 
    Details
    ISSN: 1432-2242
    Keywords: Bermudagrass ; Arbitrary primers ; DAF ; Capillary electrophoresis ; Genetic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used DNA amplification fingerprinting (DAF) to study the genetic variation of bermudagrass (Cynodon) species and cultivars of interspecific crosses that exhibit leaf-blade textural characteristics ranging from coarse to fine. Arbitrary octamer primers produced complex and reproducible amplification profiles with high levels of polymorphic DNA. Phylogenetic analysis using parsimony (PAUP) and unweighted pair group cluster analysis using arithmetic means (UPGMA) grouped 13 bermudagrass cultivars into several clusters, including one containing the African-type bermudagrasses (C. transvaalensis) and another containing the common-type bermudagrasses (C. dactylon). The latter group included C. magennissii (‘Sunturf’) and a interspecific C. transvaalensisxC. dactylon cross (‘Midiron’), 2 cultivars that exhibited leaf textural characteristics closer to the common-types. All other C. transvaalensisxC. dactylon crosses grouped between the African and common types. An extended screen of 81 octamer primers was needed to separate cultivar ‘Tifway’ from the irradiation-induced mutant ‘Tifway II’. The use of either template endonuclease digestion prior to amplification or arbitrary mini-hairpin primers increased detection of polymorphic DNA and simplified the task of distinguishing these closely related cultivars. Alternatively, the use of capillary electrophoresis (CE) resolved fingerprints adequately and detected products with high sensitivity, thereby promising to increase throughput and the detection of polymorphic DNA. When used to fingerprint samples from commercial sources, DAF identified bermudagrass plant material on the basis of unique reference profiles generated with selected primers. DAF represents an excellent technique for bermudagrass cultivar verification, seed certification, varietal protection, and for the identification of mistakes in plantings, mislabeled plant materials, and contamination or substitutions of sod fields.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220882
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  • 2
    Electronic Resource
    Electronic Resource
    Capillary GC analysis of diesel fuels using simultaneous parallel triple detection (1986)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 9 (1986), S. 39-43 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography, GC ; Fused silica capillary columns ; Parallel triple detection ; Diesel fuels ; Polyaromatic hydrocarbons ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Diesel fuels from different parts of the UK have been analyzed for polycyclic aromatic hydrocarbons (PAH), nitrogen-containing PAH, and sulfur-containing PAH using capillary column GC with simultaneous parallel triple detection. The concentrations of polyaromatic compounds (PAC) were high and showed considerable variability amongst the fuels. The PAH are mainly naphthalene, fluorene, and phenanthrene and their alkylated homologs; the PANH are mainly carbazole and its methyl derivatives; the PASH are mainly dibenzothiophene and its methyl derivatives.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240090107
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  • 3
    Electronic Resource
    Electronic Resource
    Compressibility effects in the optimization of serially connected gas chromatographic capillary columns by the window diagram technique (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 569-570 
    Details
    ISSN: 0935-6304
    Keywords: Serially coupled capillary columns ; Window diagram ; Optimization ; Compressibility ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061009
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  • 4
    Electronic Resource
    Electronic Resource
    The influence of locust bean gum and dextran on the gelation of κ-carrageenan (1996)
    New York : Wiley-Blackwell
    Biopolymers 38 (1996), S. 655-664 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The interaction of κ-carrageenan with locust bean gum and dextran has been studied by rheology, differential scanning calorimetry (DSC), and electron spin resonance spectroscopy (ESR). Rheological measurements show that the carrageenan gel characteristics are greatly enhanced in the presence of locust bean gum but not in the presence of dextran. Carrageenan/locust bean gum mixtures show two peaks in the dsc cooling curves. The higher temperature peak corresponds to the temperature of gelation and its intensity increases at the expense of the lower temperature peak as the proportion of locust bean gum in the mixture increases. Furthermore, the DSC heating curves show enhanced broadening when locust bean gum is present, indicating increased aggregation. These results are taken as evidence of carrageenan/locust bean gum association. The gelation process has also been followed by ESR using spin-labeled carrageenan. On cooling carrageenan solutions, an immobile component appears in the ESR spectra signifying a loss of segmental mobility consistent with chain stiffening due to the coil → helix conformational transition and helix aggregation. For carrageenan/locust bean gum mixtures, carrageenan ordering occurs at temperatures corresponding to the higher temperature DSC setting peak and the temperature of gelation. Similar studies using spin-labeled locust bean gum show that its mobility remains virtually unaffected during the gelation process. It is evident, therefore, that carrageenan and locust bean gum interact only weakly. It is proposed that at low carrageenan concentrations the gel network consists of carrageenan helices cross-linked by locust bean gum chains. At high carrageenan concentrations the network is enhanced by the additional self-aggregation of the “excess” carrageenan molecules. For carrageenan/dextran mixtures, only one peak is observed in the dsc cooling curves. The onset of gelation shifts to higher temperatures only at very high (20%) dextran concentrations and this is attributed to volume exclusion effects. Furthermore, there is no enhanced broadening of the peaks in the DSC heating curves as for the carrageenan/locust bean gum systems. It is therefore concluded that carrageenan/dextran association does not occur. The difference in behavior between locust bean gum and dextran is attributed to the greater flexibility of the dextran chains. © 1996 John Wiley & Sons, Inc.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Studies on κ-carrageenan/locust bean gum mixtures in the presence of sodium chloride and sodium iodide (1997)
    New York : Wiley-Blackwell
    Biopolymers 41 (1997), S. 657-671 
    Details
    ISSN: 0006-3525
    Keywords: κ-carrageenan ; κ-carrageenan-locust bean gum mixtures ; small deformation oscillation measurements ; differential scanning calorimetry ; locust bean gum ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The solution properties of κ-carrageenan and κ-carrageenan/locust bean gum mixtures have been studied by small deformation oscillation measurements and differential scanning calorimetry (DSC) in the presence of sodium chloride and sodium iodide. Both salts induced the κ-carrageenan to undergo a coil-helix conformational change as noted by an increase in the storage and loss moduli (G′, G′) and by an exothermic peak in the DSC cooling curves. The enthalpy ΔHc-h and temperature of the conformational transition Tc-h were higher in Nal compared to NaCl and Tc-h increased with increasing the concentration of both electrolytes. Gelation was not observed for carrageenan or carrageenan/locust bean gum mixtures in the presence of up to 200 mM Nal. Although carrageenan alone did not gel in the presence of 100 mM NaCl, a weak gel was obtained for a mixture containing 0.9%/0.1% carrageenan/locust bean gum. Furthermore, the mixture showed hysteresis in both the rheological and DSC cooling and heating curves. A strong gel was produced for carrageenan alone in the presence of 200 mM NaCl and the gel strength increased on adding a small proportion of locust bean gum (0.9%/0.1%). © 1997 John Wiley & Sons, Inc. Biopoly 41: 657-671, 1997
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Selective separation of proteins from mixtures using polysaccharides (1998)
    New York : Wiley-Blackwell
    Biopolymers 45 (1998), S. 97-104 
    Details
    ISSN: 0006-3525
    Keywords: selective separation of proteins from mixtures ; polysaccharides ; phase behavior ; phase separation ; depletion flocculation ; ternary systems ; quatenary systems ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The phase behavior of ternary (protein + dextran + solvent) and quaternary (protein 1 + protein 2 + dextran + solvent) aqueous solutions have been monitored at various ionic strengths and pHs by turbidity measurements. For the ternary systems, phase separation was favored when the solvent conditions promoted protein self-association. Bovine serum albumin (BSA) and γ-globulin were believed to self-associate in the presence of dextran at their respective isoelectric points through the electrostatic interactions between positive and negative charged patches at their surface. The fact that phase separation for BSA became less evident on increasing the ionic strength supported this concept. In the case of lysozyme alone, self-association was promoted by the addition of electrolyte as was phase separation in the lysozyme + dextran ternary system. For this biopolymer self-association is presumably as a consequence of van der Waals and/or hydrophobic forces. The influence of dextran has been discussed in terms of polymer depletion theory where an additional attractive force is created due to the exclusion of the dextran molecules from the immediate vicinity of the protein surface. For the quaternary systems, by subtle choice of solvent conditions, BSA and globulin could be exclusively partitioned into separate liquid layers through a segregative mechanism, while BSA and lysozyme could be separated into a single highly concentrated layer through an associative mechanism. © 1998 John Wiley & Sons, Inc. Biopoly 45: 97-104, 1998
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Accurate molecular weight distribution of polymers using thermal field-flow fractionation with deconvolution to remove system dispersion (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 37 (1989), S. 2059-2076 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Thermal field-flow fractionation (ThFFF) is an elution process that separates polymeric materials by molecular weight. Elution profiles thus provide approximations to the molecular weight distributions of polymers. The accuracy of such approximate distributions is expected to be improved by accounting for the effect on the elution profile of band-broadening processes in the FFF system. Fortunately this intrinsic band broadening, referred to as system dispersion, is theoretically well-defined in ThFFF. In this article we present an algorithm that corrects ThFFF elution profiles by removing system dispersion. The program is applied to ThFFF fractograms of standard polymers having both narrow and broad molecular weight distributions. The increased accuracy obtained by accounting for system dispersion is demonstrated. For the narrow standard, deconvolution shows that the polydispersity (weight/number-average mol. wt.) is only 1.004. For the broad standard, NBS 706, the molecular weight distribution and parameters obtained agree well with previously published results. Application to a simulated fractogram resulting from mixing five narrow standards helps define the conditions under which accurate molecular weight information can be recovered.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1989.070370725
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  • 8
    Electronic Resource
    Electronic Resource
    The status of reference data, reference materials and reference procedures in surface analysis (1988)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 13 (1988), S. 46-50 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: A brief synopsis is given of recent developments and current needs for reference data, reference materials and reference procedures in surface analysis. This assessment is based largely on three presentations and related discussion at the Second Topical Conference on Quantitative Surface Analysis held at Monterey, California on 30-31 October, 1987. While a reasonable start has been made in recent years in providing needed data, materials, and procedures, many important needs remain.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740130110
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  • 9
    Electronic Resource
    Electronic Resource
    XPS Evidence for the Incongruent Surface Oxidation of Minerals in the Pd-Te-Bi System (1996)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 24 (1996), S. 641-646 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Selected members of the Pd-Te-Bi system (PdTe, PdTeBi and PdBi) have been synthesized from their constituent elements, their structure and phase integrity confirmed by x-ray diffraction and optical microscopy and their initial oxidation in air under ambient conditions investigated by means of XPS. Prior to air exposure, the Pd core-electron binding energies were 0.6-1.0 eV higher than the corresponding values for the pure metal, while the core-level shifts for the alloying elements were very small. In each case, oxidation was quite rapid, but the palladium did not appear to be directly involved in the initial reaction; no change in the Pd 3d spectrum other than in its intensity was observed, notwithstanding the formation of significant amounts of tellurium oxide and/or bismuth oxide. The electron spectra were consistent with an incongruent oxidation reaction leading to the formation of a layer of tellurium and/or bismuth oxide covering a palladium-enriched substrate.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Single strand conformational polymorphism using capillary electrophoresis with two-dye laser-induced fluorescence detection (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1429-1436 
    Details
    ISSN: 0173-0835
    Keywords: Capillary electrophoresis ; Mutation detection ; Single strand conformational polymorphism ; Two-dye laser-induced fluorescence ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Capillary electrophoresis (CE), using a replaceable polymer matrix and two-dye laser-induced fluorescence has been applied to single strand conformational polymorphism (SSCP). Two-dye laser-induced fluorescence has been used for improved strand identification over a single-dye approach. Conditions suitable in the capillary format for rapid separation and high resolution have been explored. The influence of separation parameters such as temperature and matrix composition on separation in SSCP was first determined. Short analysis times allowed for fast screening of optimal separation conditions of the sample. Based on these results, the two strands of a standard 255 bp fragment of the lacI gene were resolved within 25 min with replaceable linear polyacrylamide as a separation matrix. The method was then applied to the detection of different mutations, in the presence of wild type, of a 276 bp fragment of the insulin-like growth factor 1-binding protein 1 (IGF1-BP3) gene.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601236
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