ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

Digitale Medien

Computer graphics of SEM images facilitate recognition of chromosome position in isolated human metaphase plates (1995)

Hodge, L. D. ; Barrett, J. M. ; Welter, D. A.

New York, NY [u.a.] : Wiley-Blackwell

Microscopy Research and Technique 30 (1995), S. 408-418

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1059-910X

Schlagwort(e): Mitosis ; Chromosomes ; Lung cells ; HeLa S3 ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Notizen: There is general agreement that at the time of mitosis chromosomes occupy precise positions and that these positions likely affect subsequent nuclear function in interphase. However, before such ideas can be investigated in human cells, it is necessary to determine first the precise position of each chromosome with regard to its neighbors. It has occurred to us that stereo images, produced by scanning electron microscopy, of isolated metaphase plates could form the basis whereby these positions could be ascertained. In this paper we describe a computer graphic technique that permits us to keep track of individual chromosomes in a metaphase plate and to compare chromosome positions in different metaphas plates. Moreover, the computer graphics provide permanent, easily manipulated, rapid recall of stored chromosome profiles. These advantages are demonstrated by a comparison of the relative position of group A - specific and groups D - and G - specific chromosomes to the full complement of chromosomes in metaphase plates isolated from a nearly triploid human-derived cell (HeLa S3) to a hypo-diploid human fetal lung cell. © 1995 Wiley-Liss, Inc.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1070300507

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

2

Digitale Medien

Electrochemical and microstructural study of oxide films formed electrochemically at microcrystalline al-fe-v-si alloys (1995)

Thomas, S. C. ; Birss, V. I. ; Steele, D. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Microscopy Research and Technique 31 (1995), S. 285-292

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1059-910X

Schlagwort(e): Oxide film ; Barrier film ; Ultramicrotomy ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Notizen: A recent advance in metallurgical technology has been the application of rapid solidification techniques to Al alloy production. FVS0812 is the designation given to a microcrystalline Al-based alloy consisting of 8 wt% Fe, 1 wt% V and 2 wt% Si. It is a two-phase alloy, consisting of ca. 27 vol percent of approximately spherical Fe-V-Si-rich dispersoids in an essentially pure Al matrix. The high strength, low density properties of this advanced material, and other related alloys, have not yet been realized, however, due, in part, to the inability of the alloy to form a thick, adherent, abrasion-resistant outer surface oxide film, a feature readily achieved at conventional Al alloys by normal anodizing methods. The present research has involved an electro-chemical study of oxide film growth at the 812 alloy, with the specific goals being to seek an understanding of the origin of the oxide film growth problem and ultimately to propose alternative approaches to the formation of a thick, stable oxide film at this material. The techniques used in this research have included electrochemical methodologies such as cyclic voltammetry and electrochemical impedance spectroscopy. Crucial information has been obtained through transmission electron microscopy (TEM) of ultramicrotomed specimens. Experiments were carried out initially in neutral borate solutions to characterize the compact barrier oxide film formed in this environment and expected to be present beneath the porous oxide film formed in the normal sulfuric acid anodizing medium. In borate solutions, the electrochemical results implied oxide film thicknesses which were less than seen subsequently by TEM work, suggesting either that the barrier film at the 812 alloy can be penetrated by solution in very fine pores (not resolvable by conventional TEM) at its outer surface or that dispersoids trapped in the oxide film cause differential oxide film thicknesses to develop across the alloy surface. In sulfuric acid solutions, dissolution of Fe and V occurs from the 812 alloy during anodization. Both impedance and TEM studies reveal the absence of a barrier film at the 812 alloy surface. Also, the thick oxide overlayer has a tortuous and more open pore structure than formed at Al and the oxide film is also substantially thinner than it should be. It is suggested that the absence of a barrier oxide film indicates that the sulfuric acid anodizing medium is too aggressive for oxide film formation at the 812 alloy, resulting in excessive dissolution and poor oxide film qualities. © 1995 Wiley-Liss, Inc.

Zusätzliches Material: 9 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1070310405

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

3

Digitale Medien

Scanning electron microscopy of the human esophagus: Application to barrett's esophagus, a precancerous lesion (1995)

Shields, H. M. ; Sawhney, R. A. ; Zwas, F. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Microscopy Research and Technique 31 (1995), S. 248-256

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1059-910X

Schlagwort(e): Esophageal reflux ; Morphometry ; Distinctive cell ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Notizen: In Barrett's esophagus, metaplastic columnar epithelium replaces the normal squamous epithelium. The importance of this lesion lies in the increased incidence of adenocarcinoma of the esophagus occuring in patients with Barrett's esophagus. We characterized the surface epithelial cells of Barrett's esophagus using quantitative scanning electron microscopy. Three distinct surface cell types, in addition to the goblet cell, were recognized in Barrett's epithelium: the gastric-like cell and the intestinal-like cell, both of which were similar to normal gastric and small intestinal surface cells, respectively, by quantitative scanning electron microscopy, and the variant cell which had a range of surface features. In four biopsy specimens from the squamo-Barrett's junction in three patients, we found the distinctive cell that had features intermediate between those of squamous and columnar epithelium. On the distinctive cell's surface there are two disparate structures not normally present on the same cell in the gastrointestinal tract: microvilli (a scanning electron microscopy feature of glandular epithelium) and intercellular ridges (a scanning electron microscopy feature of squamous epithelium). The surface characteristics of this cell were almost identical to those of cells found in the transformation zone of the uterine cervix, an area in which squamous epithelium physiologically replaces columnar epithelium. Scanning electron microscopy of Barrett's esophagus has increased our understanding of this precancerous lesion by showing striking cellular heterogeneity. It has also identified the distinctive cell which may represent an intermediate step in the development of Barrett's epithelium during which the surface characteristics of two different cell types, columnar and squamous, coexist in the same cell. © 1995 Wiley-Liss, Inc.

Zusätzliches Material: 9 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1070310308

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

4

Digitale Medien

A simple device for the preparation of embedded materials science specimens for ultramicrotomy (1987)

Blake, D. F. ; Bunch, T. E. ; Philpott, D. E. ; [weitere]

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 6 (1987), S. 305-306

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0741-0581

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1060060304

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

5

Digitale Medien

An inexpensive detector for water-vapour in the low temperature scanning electron microscope (1989)

Lawton, D. M. ; Gardner, D. L. ; Oates, K. ; [weitere]

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 11 (1989), S. 90-91

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0741-0581

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Zusätzliches Material: 2 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1060110112

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

6

Digitale Medien

Preparation of biological tissue sections for correlative ion, electron, and light microscopy (1984)

Kupke, K. G. ; Pickett, J. P. ; Ingram, P. ; [weitere]

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 1 (1984), S. 299-309

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0741-0581

Schlagwort(e): Electron microscopy ; Ion microscopy ; Correlative microscopy ; Electron probe microanalysis ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Notizen: In order to correctly interpret the chemical images obtained using ion microscopy (IM), it is useful to correlate them with the information provided by conventional light microscopy (LM), secondary electron imaging (SEI), backscattered electron imaging (BEI), and electron probe microanalysis (EPMA). Accordingly, we have devised a technique of specimen preparation which allows for the application of several different microanalytical techniques to a single histologic section mounted on the same substrate. Sections are cut onto polyester plastic coverslips (devoid of peaks for any element with atomic number 〉 9 using EPMA) and studied by LM. After a light rotary coating with carbon (to prevent charging), the section can then be examined by SEI, BEI, and EPMA. Specific areas can be marked for IM study either with an objective-mounted pin tissue microlocater, or by placing small pieces of metal foil, cut in specific geometric shapes, over features of interest. After sputter-coating the sample with platinum, metal-free shadows are visible using a low-power reflected light microscope available on a typical IM sample chamber as a guide for ion beam placement. The conductive coatings also minimize specimen charging during IM. Post-IM light microscopy, SEI, and BEI are used to confirm the location of specific areas probed in the IM experiments and to provide information on differential ion-sputtering artifacts and tissue contaminants. This new correlative technique should permit better understanding of the images obtained with these diverse instruments.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1060010309

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

7

Digitale Medien

High-resolution electron microscopy of high-temperature superconductors (1988)

Marks, L. D. ; Li, D. J. ; Shibahara, H. ; [weitere]

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 8 (1988), S. 297-306

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0741-0581

Schlagwort(e): Defects ; Polymorphs ; Structure ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Notizen: We review recent results obtained at Northwestern using high-resolution electron microscopy to study high-temperature superconductors. While in general these materials form large, very perfect single crystal grains which display very few imperfections, there is also evidence of slip defects, amorphous regions, and order-disorder transformations. We also report that the gadolinium-based superconductors and in one case yttrium-based superconductors show evidence for some copper solid solubility in the form of copperrich planar defects. The structure of a metastable trigonal polytype is also reported, as are the effects of electron beam and water vapor damage to the materials.

Zusätzliches Material: 9 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1060080308

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

8

Digitale Medien

A method of shipping cell monolayers on glass cover slips for sem (1986)

Engelkirk, P. G. ; Paine, D. D. ; Koester, S. K. ; [weitere]

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 3 (1986), S. 371-372

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0741-0581

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1060030315

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

9

Digitale Medien

Postembedding colloidal-gold immunocytochemistry of noncollagenous extracellular matrix proteins in mineralized tissues (1995)

McKee, M. D. ; Nanci, A.

New York, NY [u.a.] : Wiley-Blackwell

Microscopy Research and Technique 31 (1995), S. 44-62

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1059-910X

Schlagwort(e): Mineralization ; Bone ; Cartilage ; Cementum ; Dentin ; Enamel ; Osteopontin ; Osteocalcin ; Bone sialoprotein ; Amelogenin ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Notizen: Immunocytochemistry is a powerful tool for investigating protein secretion, extracellular matrix assembly, and cell-matrix and matrix-matrix/mineral relationships. When applied to the tissues of bones (bone and calcified cartilage) and teeth (dentin, cementum, and enamel), where calcium phosphate-containing extracellular matrices are the predominant structural component related to their weight-bearing and masticatory roles, respectively, data from immunocytochemical studies have been prominent in advancing our understanding of mineralized tissue modeling and remodeling. The present review on the application of postembedding, colloidal-gold immunocytochemistry to mineralized tissues focuses on the advantages of this approach and relates them to conceptual, theoretical, and experimental data currently available discussing matrix-mineral interactions and extracellular matrix formation and turnover in these tissues. More specifically, data are summarized regarding the distribution and role of noncollagenous proteins in different mineralized tissues, particularly in the context of how they interface with mineral, and how this relationship might be affected by the various tissue-processing steps and immunocytochemical strategies commonly implemented to examine the distribution and function of tissue proteins. Furthermore, a technical discussion is presented that outlines several different possibilities for epitope exposure in mineralized tissues during preparation of thin sections for transmission electron microscopy. Cell biological concepts of protein secretion by cells of the mineralized tissues, and subsequent extracellular matrix assembly and organization, are illustrated by examples of high-resolution, colloidal-gold immunolabeling for osteopontin, bone sialoprotein, and osteocalcin in the collagen-based mineralized tissues and for enamel protein (amelogenin) in enamel. © 1995 Wiley-Liss, Inc.

Zusätzliches Material: 9 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1070310105

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

10

Digitale Medien

Morphological study of changes in the baboon oviductal epithelium during the menstrual cycle (1995)

Louise Odor, D. ; Augustine, James R.

New York, NY [u.a.] : Wiley-Blackwell

Microscopy Research and Technique 32 (1995), S. 13-28

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1059-910X

Schlagwort(e): Ciliated and secretory cells as related to menstrual cycle ; LM ; SEM ; TEM ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Notizen: Oviductal epithelium of the baboon, Papio cynocephalus, was studied utilizing light, scanning, and transmission electron microscopy. Results of counts made of nonciliated, ciliated, and ciliogenic cells were analyzed statistically. The percentages of nonciliated cells of the fimbria and ampulla during the early proliferative and late secretory stages of the menstrual cycle were significantly greater than those during the mid-proliferative and late proliferative-early secretory stages, due to deciliation. This paper emphasizes previously unreported apical surface morphology as viewed by scanning and transmission electron microscopy. The microvillar pattern of the fimbrial secretory cells differs from that of the ampullar and isthmic cells in that the microvilli originate from thick apical protrusions and vary greatly in length and number as related to the cycle. A ridge demarcating the apical intercellular junction is composed of rows of microvilli during the early proliferative and late secretory stages. During the early proliferative and late secretory stages an increased degree of invagination of the basal and lateral plasma membranes occurs as the height and width of the cells decreases. The general numbers and distribution of the organelles of the various types of oviductal cells agree with that described for the ampulla and isthmus by Verhage et al. [(1990) Am. J. Anat., 187:81-90]; however, fimbrial epithelium was not included in that study. Other cyclic ultrastructural changes not examined previously include variation in the number of lipid droplets and their location, and in the number and relationships of glycogen particles to other structures. © 1995 Wiley-Liss, Inc.

Zusätzliches Material: 22 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1070320103

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext