ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
An extracellular amyloglucosidase (56 kDa) of Termitomyces clypeatus which was accumulated intracellularly in absence of Krebs cycle acids in dextrin medium, remained mostly inside the plasma membrane. The enzyme was localised in mycelial vacuoles as a sucrase-amyloglucosidase aggregate. The intracellular pool contained both the free sucrase and the aggregate, while vacuoles contained only aggregate without any free enzyme. The aggregate, partially purified to PAGE homogeneity, contained amyloglucosidase and sucrase in the ratio of 1:1. A cellular regulation, influenced by the presence of Krebs cycle acids, was indicated to be present at the level of exocytosis of vacuolar enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1998.tb12807.x
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