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  • Flagella  (4)
  • Arabidopsis  (2)
  • 1995-1999  (2)
  • 1985-1989
  • 1980-1984  (4)
  • 1935-1939
  • 1
    Electronic Resource
    Electronic Resource
    An antiserum against a glycoprotein functional in flagellar adhesion between Chlamydomonas eugametos gametes (1982)
    Springer
    Archives of microbiology 131 (1982), S. 241-246 
    Details
    ISSN: 1432-072X
    Keywords: Chlamydomonas ; Agglutination ; Flagella ; Glycoproteins ; Anti-PAS-1.2 serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlamydomonas eugametos gametes agglutinate sexually by their flagellar surfaces. The agglutination factor on mating type minus (mt-) gametes is thought to be a glycoprotein named PAS-1.2. To test this idea, an antiserum was raised against purified PAS-1.2., which reacted with isolated PAS-1.2 (immunoprecipitation tests) and blocked the ability of isolated PAS-1.2 to induce sexual twitching in mt + gametes. When tested with living cells, the antiserum specifically agglutinated mt - gametes and induced a reaction resembling twitching. Mt + flagella were shown to bind the antiserum (indirect immunofluorescence) but much less than mt - gametes. Mt - gametes pretreated with Fab fragments of the antiserum were unable to reproduce sexually, while treated mt + gametes were unaffected. This effect presumably results from the ability of the serum to block mt - sexual agglutination, for mt - isoagglutinin was completely inactivated by the serum, while mt + isoagglutinin was unaffected. It is therefore argued that PAS-1.2 is the in vivo mt - agglutination factor. However it is shown that the antiserum was able to react in vitro not only with PAS-1.2 but with several other proteins in both mt - and mt + flagella.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405886
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of an antigenic determinant from flagellar glycoproteins of Chlamydomonas eugametos (1983)
    Springer
    Archives of microbiology 135 (1983), S. 311-314 
    Details
    ISSN: 1432-072X
    Keywords: Chlamydomonas ; Sexual reproduction ; Agglutination ; Flagella ; Antigenic determinant ; Glycoproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A labeled oligosaccharide fraction, obtained by reductive β-elimination of an extract of minus mating-type flagella of Chlamydomonas eugametos, is shown to contain a disaccharide fraction which is bound by an antiserum raised against the sexual agglutination factor present at the flagellar surface of that species. The fraction contains arabinose and galactose. It is argued that galactose and/or arabinose may be a functional constituent of the agglutination factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413487
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  • 3
    Electronic Resource
    Electronic Resource
    Sex-specific glycoproteins in Chlamydomonas flagella an immunological study (1980)
    Springer
    Archives of microbiology 126 (1980), S. 77-81 
    Details
    ISSN: 1432-072X
    Keywords: Chlamydomonas ; Sex ; Flagella ; Agglutination ; Glycoproteins ; Immunochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To identify mating type-specific glycoproteins associated with the flagellar membrane of Chlamydomonas eugametos, which could be involved in sexual agglutination, antibodies were raised in rabbits against purified gamete flagella of either mating type. The immunoglobulin (Ig) fractions exhibited partial mating-type specificity in agglutinating gametes, in the indirect immunofluorescence test and in the crossed immunoelectrophoresis test. This specificity was strongly enhanced by absorbing the fractions with flagella of the opposite mating type. Absorbed Ig fractions produced a single precipitation line with Triton extracts of gamete flagella in the crossed immunoelectrophoresis technique. On polyacrylamide gel electrophoresis this line appeared to contain two flagellar glycoprotein fractions, PAS 1 and PAS 4. Polyacrylamide gels of flagellar extracts incubated with these Ig fractions, followed by staining with peroxidase-anti-rabbit Ig resulted in the staining of only the PAS 1 and PAS 4 bands, which confirms that these components of the flagellar membrane are mating type-specific antigens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421894
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  • 4
    Electronic Resource
    Electronic Resource
    Modification of reproductive development in Arabidopsis thaliana under spaceflight conditions (1996)
    Springer
    Planta 198 (1996), S. 588-594 
    Details
    ISSN: 1432-2048
    Keywords: Arabidopsis ; Female gametophyte ; Pollen ; Reproduction ; Spaceflight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Reproductive development in Arabidopsis thaliana (L.) Heynh. cv. Columbia plants was investigated under spaceflight conditions on shuttle mission STS-51. Plants launched just prior to initiation of the reproductive phase developed flowers and siliques during the 10-d flight. Approximately 500 flowers were produced in total by the 12 plants in both the ground control and spaceflight material, and there was no significant difference in the number of flowers in each size class. The flower buds and siliques of the spaceflight plants were not morphologically different from the ground controls. Pollen viability tests immediately post-flight using fluorescein diacetate indicated that about 35% of the pollen was viable in the spaceflight material. Light-microscopy observations on this material showed that the female gametophytes also had developed normally to maturity. However, siliques from the spaceflight plants contained empty, shrunken ovules, and no evidence of pollen transfer to stigmatic papillae was found by light microscopy immediately post-flight or by scanning electron microscopy on fixed material. Short stamen length and indehiscent anthers were observed in the spaceflight material, and a film-like substance inside the anther that connected to the tapetum appeared to restrict the release of pollen from the anthers. These observations indicate that given appropriate growing conditions, early reproductive development in A. thaliana can occur normally under spaceflight conditions. On STS-51, reproductive development aborted due to obstacles in pollination or fertilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262646
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  • 5
    Electronic Resource
    Electronic Resource
    Sexual agglutination factor from Chlamydomonas eugametos (1981)
    Springer
    Planta 153 (1981), S. 362-369 
    Details
    ISSN: 1432-2048
    Keywords: Agglutination (sexual) ; Chlamydomonas ; Flagella ; Glycoprotein and sexual agglutination ; Membrane ; Sexual reproduction (Chlamydomonas)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlamydomonas eugametos gametes can sexually agglutinate via their flagellar surfaces whereas vegetative cells cannot. Therefore, flagellar glycoproteins, present in gamete cells but absent from vegetative cells, were investigated as prospective mt -agglutination factors. They were identified as periodic acid Schiff (PAS) stained bands separated in sodium dodecyl sulphate-polyacrylamide electrophoresis gels. Gamete-specific bands were determined by comparison with equivalent gels of vegetative flagella and by immunological techniques using antisera raised against isolated mt - gamete flagella. Four high molecular weight flagellar glycoproteins proved to be gamete specific (PAS-1.2, PAS-1.3, PAS-3 and PAS-4). They were extracted from flagella by 3 M guanidine thiocyanate, separated in a column of Sepharose 2B, and tested for in vitro agglutination activity on mt + gametes. A single peak of activity was found to be correlated with the presence of the PAS-1.2 band. It is shown that mt - agglutination activity is related to the concentration of this glycoprotein in flagellar membranes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384255
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  • 6
    Electronic Resource
    Electronic Resource
    Dynamics of vegetative cytoplasm during generative cell formation and pollen maturation inArabidopsis thaliana (1996)
    Springer
    Protoplasma 194 (1996), S. 81-90 
    Details
    ISSN: 1615-6102
    Keywords: Arabidopsis ; Pollen ; Vegetative cytoplasm ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultrastructural changes of pollen cytoplasm during generative cell formation and pollen maturation inArabidopsis thaliana were studied. The pollen cytoplasm develops a complicated ultra-structure and changes dramatically during these stages. Lipid droplets increase after generative cell formation and their organization and distribution change with the developmental stage. Starch grains in amyloplasts increase in number and size during generative and sperm cell formation and decrease at pollen maturity. The shape and membrane system of mitochondria change only slightly. Dictyo-somes become very prominent, and numerous associated vesicles are observed during and after sperm cell formation. Endoplasmic reticulum appears extensively as stacks during sperm cell formation. Free and polyribosomes are abundant in the cytoplasm at all developmental stages although they appear denser at certain stages and in some areas. In mature pollen, all organelles are randomly distributed throughout the vegetative cytoplasm and numerous small particles appear. Organization and distribution of storage substances and appearance of these small particles during generative and sperm cell formation and pollen maturation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273170
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