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  • 1
    Electronic Resource
    Electronic Resource
    Physicochemical properties of (1 → 6)-branched (1 → 3)-β-D-glucans. 1. Physical dimensions estimated from hydrodynamic and electron microscopic data (1993)
    New York : Wiley-Blackwell
    Biopolymers 33 (1993), S. 561-573 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The physical dimensions of several (1 → 6) branched (1 → 3) -β-D-glucan samples obtained from different organisms and their derivatives have been studied by electron microscopy, light scattering measurements, viscometry, and gel permeation chromatography. The electron micrographs indicate that in most samples these biopolymers are adequately described as linear worm-like coils. A sample reconstituted from alkaline media appeared as a blend of the linear, circular, and aggregated polymer morphologies. The average mass per unit length, ML = Mw/Lw for the macroscopically linear samples, was estimated to be 2100 ± 200 g mol-1 nm-1. The parameter mL was determined from the contour lengths obtained by electron microscopy and the molecular weight by light scattering measurements. The observed ML was consistent with the triple-helical structure reported from x-ray diffraction studies and observed degree of side-chain substitution. From the molecular snapshots shown in the electron micrographs, the persistence lengths of these β-D-glucans were determined to be 140 ± 30 nm. The experimentally determined intrinsic viscosities were consistent with these estimates of ML and persistence length. Comparison of the molecular weight distributions obtained from gel permeation chromatography and those deduced from the electron micrographs indicates that number and weight average contour lengths are more reliable than z and z + 1 averages. © 1993 John Wiley & Sons, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360330406
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  • 2
    Electronic Resource
    Electronic Resource
    Novel synthesis of aromatic polysulfides from S,S′-bis(trimethylsilyl)-substituted aromatic dithiols and activated aromatic dihalides (1991)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 29 (1991), S. 1933-1940 
    Details
    ISSN: 0887-624X
    Keywords: new synthetic method ; aromatic polysulfides ; S,S′-bis (trimethylsilyl) dithiols ; aromatic dihalides ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A new method for the synthesis of aromatic polysulfides has been developed by the polycondensation of S,S′-bis(trimethylsilyl)-substituted aromatic dithiols with activated aromatic dihalides. The solution polycondensation of three S-silylated aromatic dithiols with bis(4-chloro-3-nitrophenyl) sulfone afforded readily aromatic polysulfides having inherent viscosities of 0.7 dL/g, and the polymerization with bis(4-fluorophenyl) sulfone gave the polymers with viscosity values of 0.3 dL/g. The silylation method was compared advantageously with a conventional route using parent dithiols and activated aromatic dihalides.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1991.080291313
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  • 3
    Electronic Resource
    Electronic Resource
    Chemiluminescent immunoassay (CLIA) for salmon growth hormone (GH) (1997)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 12 (1997), S. 271-275 
    Details
    ISSN: 0884-3996
    Keywords: chemiluminescent immunoassay ; acridinium ester ; fish ; salmon ; growth hormone ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A highly sensitive and specific chemiluminescent immunoassay (CLIA) was developed for quantification of growth hormone (GH) in salmonid species. The CLIA for salmon GH was performed using the sandwich method with anti-GH IgG as the first antibody and chemiluminescent acridinium ester-labelled specific anti-GH F(ab′)2 as the second antibody. The measurable range of salmon GH in the CLIA was 39-1250 pg/mL using a short assay (1 day) protocol and 3.9-125 pg/mL in a longer (2-day) assay. The dilution curve in the CLIA of serum from masu salmon (Oncorhynchus masou) was parallel to the standard curve of recombinant chum salmon (Oncorhynchus keta) GH. Seasonal changes of serum GH levels were measured in 1 year-old masu salmon cultivated in a pond from March to November. Their serum GH levels increased during smoltification from March to April, achieved a maximum level of 21 ng/mL in August, and then declined gradually to 11 ng/mL in October. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Water absorption effects on the thermal transition and stiffness of ethylene ionomers (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 55 (1995), S. 131-138 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Changes in the thermal transition and stiffness of ionomers occuring during aging or water absorption were investigated. Ionomers used in this study are Na, K, Mg, and Zn salts of poly(ethylene-co-methacrylic acid) (EMAA), whose methacrylic acid unit is 5.4 mol %. It was found that ionomer stiffness is subject to water absorption and the effect differs with the neutralizing cations. Water absorption effects on the thermal transition, measured by differential scanning calorimetry (DSC), indicate that there are no significant changes in the higher-temperature endotherm assigned to a melting of polyethylene (PE) crystallities. However, the lower-temperature endotherm, which we attribute to an order-disorder transition of ionic aggregates (Ti), exhibits significant changes upon water absorption. The mechanism of the water absorption effects on the thermal transition and stiffness is described. © 1995 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070550115
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  • 5
    Electronic Resource
    Electronic Resource
    Recognition of amino acids by membrane potential of immobilized globulin membranes (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 55 (1995), S. 343-349 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The shifts in membrane potential, caused by the injection of some amino acids into a permeation cell, were measured using immoblized γ-globulin membranes. The shifts in membrane potential were observed to be positive or negative when the isoelectric point of each amino acid injected into the cell was less or higher than 6.0. The potential response caused by the injection of each amino acid shows an individual and characteristic curve depending on the amino acid, and the difference in potential curves between D-aspartic acid and L-aspartic acid is significantly observed in the immobilized γ-globulin membranes. The t3/4 value was found to increase in the following order: lysine = glutamic acid 〈arginine 〈 D-aspartic acid = asparagine 〈 L-aspartic acid 〈 histidine 〈 alanine, where t3/4 indicates the time at which 75% of the shifts in membrane potential has been observed. The modified membrane potential theory provides satisfactory explanations for the membrane potential obtained experimentally before and after the injection of L-alanine, and the theoretical shifts can explain the experimental shifts in membrane potential due to the injection of L-alanine into the cell. © 1995 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070550216
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  • 6
    Electronic Resource
    Electronic Resource
    Immune complex transfer two-site chemiluminescent immunoassay for serum growth hormone in alevin chum salmon (1998)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 13 (1998), S. 107-111 
    Details
    ISSN: 0884-3996
    Keywords: chemiluminescent immunoassay ; acridinium ester ; fish ; salmon ; growth hormone ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: An immune complex transfer two-site chemiluminescent immunoassay (CLIA) for salmon growth hormone (GH) was developed to measure serum GH in alevin chum salmon (Oncorhynchus keta) using a chemiluminescent acridinium ester as a label. The immune complex transfer method dramatically reduced non-specifically bound of acridinium ester-labelled antibody without a decrease in the specific binding. Consequently, we could detect lower levels of GH than achieved previously in a two-site CLIA for salmon GH. The detection limit of the assay was 7.8 fg/mL and the standard curve was linear up to 250 fg/mL. Coefficients of variation were 2.2-7.7% within-assay and 5.3-9.1% between-assay. We have developed a highly sensitive and reproducible GH method and applied it to measurement of GH in alevin chum salmon. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Mechanism and process of chemomechanical contraction of polyelectrolyte gels under electric field (1990)
    New York, NY : Wiley-Blackwell
    Polymers for Advanced Technologies 1 (1990), S. 19-25 
    Details
    ISSN: 1042-7147
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: When a water-swollen polyelectrolyte gel is interposed between a pair of electrodes and DC current is applied, the gel undergoes electro-chemomechanical contraction and concomitant water exudation both in water and in the air. In order to clarify the mechanism of the phenomenon, contractile experiments were carried out under various conditions and led to the conclusion that the contraction is essentially associated with electrically driven movement of hydrated micro- and macroions in the gel (electrokinetic process).
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pat.1990.220010104
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  • 8
    Electronic Resource
    Electronic Resource
    Paper form copper ion conductive solid electrolyte (1993)
    New York, NY : Wiley-Blackwell
    Polymers for Advanced Technologies 4 (1993), S. 194-197 
    Details
    ISSN: 1042-7147
    Keywords: Solid electrolyte ; Copper ion conductor ; Composite ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A paper form solid electrolyte (PFSE) with good conductivity and flexibility was developed. PFSE is made of an inorganic-organic composite consisting of a copper ion conductive solid electrolyte of Rb4Cu16I7Cl13 (SE) and a polymer binder embedded in a synthetic fibre network. A PFSE with styrene-butadiene based synthetic rubber (SB) was found to give the highest conductivity among the binding materials that we examined. The SB content was changed from 10 vol% to 90 vol% in the PFSE, and the electrical properties and the damp resistance of PFSE were evaluated. Electrical conductivity decreased slightly with an SB content below 70 vol% and abruptly above 70 vol%. The damp resistance of PFSE improved with the increase of the SB content and was superior to that of the raw powder. The PFSE with 65 vol% SB showed relatively high conductivity, 3 × 10-3 S/cm, which was sufficient for battery use, and good damp resistance.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pat.1993.220040220
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  • 9
    Electronic Resource
    Electronic Resource
    A selective synthesis of 3-alkynl perflouroalkyl ketones by the trifluoroborane etherate mediated 1,4-addition reaction of (1-alkynyl)diisopropoxyboranes to α,β-unsaturated ketonesDedicated to Professor Yao-Zeng Huang on the occasion of his eightieth birthday. (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Heteroatom Chemistry 3 (1992), S. 483-486 
    Details
    ISSN: 1042-7163
    Keywords: Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A variety of 3-alkynyl perfluoroalkyl ketones were prepared by a trifluoroborane etherate mediated 1,4-addition reaction of (l-alkynyl)diisopropoxyboranes to α,β-unsaturated ketones substituted by a perfluoroalkyl group. The undesired side reaction, such as 1,2-addition of alkynyl groups, could be avoided completely and the products were obtained in high yields.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hc.520030506
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  • 10
    Electronic Resource
    Electronic Resource
    Determination of kerosene and light oil components in blood (1991)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 20 (1991), S. 493-497 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A sensitive and rapid method to analyse fuel components in blood from rats exposed to kerosene or light oil vapour was developed by making use of capillary gas chromatography/mass spectrometry. The aliphatic hydrocarbons with carbon numbers 8-10 and aromatics such as toluene, xylene, 3- and 4-ethyltoluene and trimethylbenzenes were clearly detected in blood from rats exposed to kerosene or light oil vapour, using the head-space method combined with the salting-out technique. The concentration ratio of pseudocumene to toluene in blood exposed to light oil was higher than that in the case of exposure to kerosene. The lower limits of detection were 50 pg and 1 ng in toluene and pseudocumene, respectively. Our suggestion is that this method is useful in forensic investigations to detect fuel components in blood and for the purposes of differentiating kerosene and light oil in blood tissues.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200200810
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