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  • Korea  (6)
  • permeability  (5)
  • rice  (5)
  • Springer  (16)
  • American Geophysical Union (AGU)
  • 1995-1999  (10)
  • 1990-1994  (6)
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Keywords
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  • Springer  (16)
  • American Geophysical Union (AGU)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Prevalence of human foamy virus-related sequences in the Korean population (1998)
    Springer
    Journal of biomedical science 5 (1998), S. 267-273 
    Details
    ISSN: 1423-0127
    Keywords: Grave's disease ; Human foamy virus ; Korea ; Molecular epidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The possible association of human foamy virus (HFV) with human thyroid disorders such as Graves' disease (GD) has been a topic of controversy due to the inconsistent results reported by several groups of investigators. Here we report the investigation of the presence of HFV-related sequences in the Korean population. DNA was obtained from peripheral blood lymphocytes from 24 GD patients and 23 healthy blood donors and subjected to PCR amplification using three sets of nested primers derived fromgag, env, and LTR regions of the HFV genome. Contrary to previously reported studies, our analysis identified HFV-related sequences in the genomes of both healthy individuals and the GD patients. However, the nature of the HFV genome present in each group appeared to be different. We detected all 3 regions of HFV-related sequences in 29% of the HFV-positive GD patients, while no samples in the control group amplified all three regions. This suggests that the LTR may be used as a tool for screening for HFV in GD patients. Our data favor the hypothesis of a relationship between GD and the presence of HFV-related sequences, though in a complex way.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02255858
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  • 2
    Electronic Resource
    Electronic Resource
    Rice proteins that bind single-stranded G-rich telomere DNA (1998)
    Springer
    Plant molecular biology 36 (1998), S. 661-672 
    Details
    ISSN: 1573-5028
    Keywords: rice ; telomere binding protein ; single-stranded DNA binding protein ; telomerase ; telomere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this work, we have identified and characterized proteins in rice nuclear extracts that specifically bind the single-stranded G-rich telomere sequence. Three types of specific DNA-protein complexes (I, II, and III) were identified by gel retardation assays using synthetic telomere substrates consisting of two or more single-stranded TTTAGGG repeats and rice nuclear extracts. Since each complex has a unique biochemical property and differs in electrophoretic mobility, at least three different proteins interact with the G-rich telomere sequences. These proteins are called rice G-rich telomere binding protein (RGBP) and none of them show binding affinity to double-stranded telomere repeats or single-stranded C-rich sequence. Changing one or two G's to C's in the TTTAGGG repeats abolishes binding activity. RGBPs have a greatly reduced affinity for human and Tetrahymena telomeric sequence and do not efficiently bind the cognate G-rich telomere RNA sequence UUUAGGG. Like other telomere binding proteins, RGBPs are resistant to high salt concentrations. RNase sensitivity of the DNA-protein interactions was tested to investigate whether an RNA component mediates the telomeric DNA-protein interaction. In this assay, we observed a novel complex (complex III) in gel retardation assays which did not alter the mobilities or the band intensities of the two pre-existing complexes (I and II). The complex III, in addition to binding to telomeric sequences, has a binding affinity to rice nuclear RNA, whereas two other complexes have a binding affinity to only single-stranded G-rich telomere DNA. Taken together, these studies suggest that RGBPs are new types of telomere-binding proteins that bind in vitro to single-stranded G-rich telomere DNA in the angiosperms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005994719175
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  • 3
    Electronic Resource
    Electronic Resource
    Metal contamination of soils and dusts in Seoul metropolitan city, Korea (1995)
    Springer
    Environmental geochemistry and health 17 (1995), S. 139-146 
    Details
    ISSN: 1573-2983
    Keywords: Metal contamination ; Seoul ; Korea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Medicine
    Notes: Abstract To investigate the dispersion patterns and the characteristics of heavy metal contamination due to urbanisation and industrialisation, soils and dusts collected from the Seoul area were analysed for Cu, Pb, Zn and Cd. The metal concentrations in most soils and dusts are higher than the world averages. The pollution index ((Σ Metal concentrations in soils/Permissible level for metal)÷(Number of metals)) of soils and dusts is 〉 1 in most of the Seoul area, a result that concurs with the industrialisation and urbanisation index of the Seoul area. The soils are contaminated with Cu, Zn, Cd and particularly Pb. This suggests that the contamination of the soils in the Seoul area are mainly caused by vehicular emissions. The pollution index of soil is the highest in the Kuro area where Cu and Zn contamination in soils are due to the indigenous brass and bronze factories. From the discriminant analysis, the Seoul area may be partitioned into control, traffic and industrialized areas by the metal concentrations in the order of Zn 〉 Cu 〉 Pb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00126082
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  • 4
    Electronic Resource
    Electronic Resource
    A new model for impregnation mechanisms in different GF/PP commingled yarns (1994)
    Springer
    Applied composite materials 1 (1994), S. 351-371 
    Details
    ISSN: 1573-4897
    Keywords: commingled yarn ; impregnation ; consolidation ; thermoplastics ; processing ; permeability ; mechanical testing ; mathematical modelling ; compression moulding ; glass fibre bed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Impregnation mechanisms of different kinds of GF/PP commingled yarns have been studied. As the reinforcing fibres were always the same, a global description has been worked out. Two different mathematical approaches for fibre bed permeability (Kozeny-Carman and Gutowski) were compared. The constants of the applied mathematical models have to stay the same if the fibre reeinforcement and the fibre arrangement is the same. Neither the kind of matrix, nor the fibre volume content may change these constants. Differences in the degree of impregnation after the same process conditions can be only due to different sizes of fibre agglomerations, thus the initial distribution of reinforcing fibres and matrix. For an exact determination of impregnation times and conditions the exact distribution of fibres in the intermediate material and after processing has to be known. This distribution is determined by SEM microscopy and data given from the material supplier. The importance of different process parameters, such as temperature, pressure, processing time is weighted by determining the density and mechanical properties of the specimens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00568041
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  • 5
    Electronic Resource
    Electronic Resource
    A rice (Oryza sativa L.) cDNA encodes a protein sequence homologous to the eukaryotic ribosomal 5S RNA-binding protein (1993)
    Springer
    Plant molecular biology 23 (1993), S. 409-413 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; cloning ; rice ; L5 ; ribosomal 5 S RNA-binding protein ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rice (Oryza sativa L.) cDNA clone coding for the cytoplasmic ribosomal protein L5, which associates with 5 S rRNA for ribosome assembly, was cloned and its nucleotide sequence was determined. The primary structure of rice L5, deduced from the nucleotide sequence, contains 294 amino acids and has intriguing features some of which are also conserved in other eucaryotic homologues. These include: four clusters of basic amino acids, one of which may serve as a nucleolar localization signal; three repeated amino acid sequences; the conservation of glycine residues. This protein was identified as the nuclear-encoded cytoplasmic ribosomal protein L5 of rice by sequence similarity to other eucaryotic ribosomal 5 S RNA-binding proteins of rat, chicken, Xenopus laevis, and Saccharomyces cerevisiae. Rice L5 shares 51 to 62% amino acid sequence identity with the homologues. A group of ribosomal proteins from archaebacteria including Methanococcus vanniellii L18 and Halobacterium cutirubrum L13, which are known to be associated with 5 S rRNA, also related to rice L5 and the other eucaryotic counterparts, suggesting an evolutionary relationship in these ribosomal 5 S RNA-binding proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029016
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  • 6
    Electronic Resource
    Electronic Resource
    Early flowering and reduced apical dominance result from ectopic expression of a rice MADS box gene (1994)
    Springer
    Plant molecular biology 26 (1994), S. 657-665 
    Details
    ISSN: 1573-5028
    Keywords: apical dominance ; early flowering ; flower development ; MADS box ; rice regulatory gene ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Recent studies with dicot plants reveal that floral organ development is controlled by a group of regulatory factors containing the MADS domain. In this study, we have isolated and characterized a cDNA clone from rice, OsMADS1, which encodes a MADS-domain-containing protein. The OsMADS1 amino acid sequence shows 56.2% identity to AGL2 and 44,4% identity to AP1. The MADS box region was the most homologous to other MADS-domain-containing proteins. Northern blot analysis indicated that the rice MADS gene was preferentially expressed in floral organs. In situ localization studies showed that the transcript was uniformly present in young flower primordia and later became localized in palea, lemma, and ovary. Ectopic expression of OsMADS1 with the CaMV 35S promoter in transgenic tobacco plants dramatically alters development, resulting in short, bushy, early-flowering plants with reduced apical dominance. These results suggest that the OsMADS1 gene is involved in flower induction and that it may be used for genetic manipulation of certain plant species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00013751
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular cloning and characterization of RGA1 encoding a G protein α subunit from rice (Oryza sativa L. IR-36) (1995)
    Springer
    Plant molecular biology 27 (1995), S. 1119-1131 
    Details
    ISSN: 1573-5028
    Keywords: ADP-ribosylation ; G protein α subunit ; inclusion body ; rice ; signal transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone, RGA1, was isolated by using a GPA1 cDNA clone of Arabidopsis thaliana G protein α subunit as a probe from a rice (Oryza sativa L. IR-36) seedling cDNA library prepared from roots and leaves. Sequence analysis of genomic clone reveals that the RGA1 gene has 14 exons and 13 introns, and encodes a polypeptide of 380 amino acid residues with a calculated molecular weight of 44.5 kDa. The encoded protein exhibits a considerable degree of amino acid sequence similarity to all the other known G protein α subunits. A putative TATA sequence (ATATGA), a potential CAAT box sequence (AGCAATAC), and a cis-acting element, CCACGTGG (ABRE), known to be involved in ABA induction are found in the promoter region. The RGA1 protein contains all the consensus regions of G protein α subunits except the cysteine residue near the C-terminus for ADP-ribosylation by pertussis toxin. The RGA1 polypeptide expressed in Escherichia coli was, however, ADP-ribosylated by 10 μM [adenylate-32P] NAD and activated cholera toxin. Southern analysis indicates that there are no other genes similar to the RGA1 gene in the rice genome. Northern analysis reveals that the RGA1 mRNA is 1.85 kb long and expressed in vegetative tissues, including leaves and roots, and that its expression is regulated by light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020885
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation and characterization of two β-tubulin cDNA clones from rice (1994)
    Springer
    Plant molecular biology 26 (1994), S. 1975-1979 
    Details
    ISSN: 1573-5028
    Keywords: β-tubulin ; cDNA ; rice ; monocot ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two cDNA clones encoding two different β-tubulins, RTUB-1 and RTUB-2, were isolated from a rice cDNA library and their nucleotide sequences were analyzed. The deduced amino acid sequences showed amino acid sequence identity between 92% and 97% with other plant β-tubulins. Southern blot analysis using gene-specific and coding-region probes suggested that β-tubulins in rice are encoded by multigene families. The two cDNA clones represent two subfamilies of rice tubulins. RTUB-1 and RTUB-2, consisting of 3 to 4 genes and a single gene, respectively. The transcript levels of RTUB-1 and RTUB-2 genes were higher in actively elongating tissues such as etiolated shoot tissues and light-grown root tissues of four-day old seedlings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019507
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  • 9
    Electronic Resource
    Electronic Resource
    Development and Characterization of Rabbit Tracheal Epithelial Cell Monolayer Models for Drug Transport Studies (1995)
    Springer
    Pharmaceutical research 12 (1995), S. 1499-1505 
    Details
    ISSN: 1573-904X
    Keywords: tracheal epithelial cells ; air-interfaced culture ; ion transport ; drug transport ; permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. The objective of this study was to investigate how culture conditions would affect the morphological, functional, and permeability characteristics of rabbit tracheal epithelial cell layers being considered for drug transport studies. Methods. Rabbit tracheocytes were isolated by protease treatment and plated onto collagen-treated permeable supports at 1.3 × 106 cells/cm2. After 24 hr, cell layers were cultured either air-interfaced (AIC) on their apical surface or under conventional liquid covered conditions (LCC). Results. Scanning electron microscopy revealed mature cilia in AIC cell layers and ciliated cells denuded of cilia in LCC cell layers. Compared with LCC, AIC cell layers (n = 20) achieved a significantly higher peak equivalent short-circuit current (74.1 ± 6.5 vs. 51.6 ± 3.4 µA/cm2), a higher potential difference (70.9 ± 2.8 vs. 60.5 ± 3.0 mV), and a lower peak transepithelial electrical resistance (1.1 ± 0.03 vs. 1.5 ± 0.02 kohms,cm2). About 70% of the short-circuit current in AIC was amiloride-sensitive whereas 〈10% was furosemide-sensitive, similar to that found in native tissue. The corresponding values in LCC were 50% and 46%. The permeability of both AIC and LCC to lipophilic solutes (dexamethasone and propranolol) was similar, whereas the permeability of hydrophilic solutes (mannitol, sucrose, and albuterol) in AIC was only half that in LCC. Conclusions. Given the striking similarity in morphological and functional characteristics of the AIC to those in the in vivo situation, the AIC is favored as an in vitro model for future drug transport studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1016291522345
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  • 10
    Electronic Resource
    Electronic Resource
    Relations Between Stock Returns and Fundamental Variables: Evidence from a Segmented Market (1999)
    Springer
    Asia Pacific financial markets 6 (1999), S. 221-233 
    Details
    ISSN: 1573-6946
    Keywords: book-to-market ; explanatory factors ; Korea ; stock returns
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract This study examines relations between stock returns and potential explanatory factors in Korea, an important and segmented emerging market. Our results show that Korean stock returns in general and returns on stocks listed in Section 1 in particular are significantly positively related to book-to-market, sales-price, and debt-equity ratios, but not significantly related to market value of equity. Returns on stocks listed in Section 2 are, however, negatively related to market value of equity and not significantly related to the other three variables. Among the variables investigated by us, book-to-market ratio has the greatest explanatory power for stock returns and it indicates superior returns for value stocks. Our findings strengthen the international evidence of the role of book-to-market ratio in explaining stock returns by demonstrating its significance even in the segmented Korean market.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1010071921399
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