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  • Articles  (58)
  • Springer  (56)
  • Nature America Inc.  (2)
  • American Association of Petroleum Geologists
  • 1995-1999  (26)
  • 1990-1994  (32)
  • 1905-1909
  • Biology  (52)
  • Mathematics  (6)
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  • Articles  (58)
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Year
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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature America Inc.
    Nature genetics 21 (1999), S. 347-349 
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Sound is a well-understood, physical phenomenon. How we perceive sound and make sense of complex auditory stimuli is a different matter altogether. Genetic approaches, however, have provided a powerful strategy to dissect auditory function and to some extent, overcome the challenge posed by the ...
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The journal of Fourier analysis and applications 5 (1999), S. 589-597 
    ISSN: 1531-5851
    Keywords: 42C15 ; 39B99 ; multivariate ; nonhomogeneous ; refinement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We give necessary and sufficient conditions for the existence and uniqueness of compactly supported distribution solutionsf=(f 1,...,f r)T of nonhomogeneous refinement equations of the form $$f(x) = h(x) + \sum\limits_{\alpha \in A} {c_\alpha f(2x - \alpha )(x \in R^s )} $$ , where h=(h1,...,hr)Tis a compactly supported vector-valued multivariate distribution, A⊂Z+ s has compact support, and the coefficientsc α are real-valued r×r matrices. In particular, we find a finite dimensional matrix B, constructed from the coefficientsc α of the equation (I−B)q=p, where the vectorp depends on h. Our proofs proceed in the time domain and allow us to represent each solution regardless of the spectral radius of P(0):=2−s∑c α , which has been a difficulty in previous investigations of this nature.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 6 (1990), S. 53-59 
    ISSN: 1476-5535
    Keywords: Bituminous coal ; Biosolubilization ; Penicillium sp. ; Surface colonization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary APenicillium sp. previously shown to grow on lignite coals degraded an air-oxidized bituminous coal (Illinois #6) to a material that was more than 80% soluble in 0.5 N NaOH. Scanning electron microscopy of the oxidized Illinois #6 revealed colonization of the surface by thePenicillium sp., production of conidia, and erosion of the coal surface. The average molecular weight (MW) of Illinois #6 degraded by the fungus and base-solubilized was approximately 1000 Da. The average MW for base-solubilized Illinois #6 that was not exposed to the fungus was 6000 Da, suggesting solubilizing mechanisms other than base catalysis. A spectrophotometric assay to quantify the microbial conversion of biosolubilized coal was developed. Standard curves were constructed based on the absorbance at 450 nm of different quantities of microbe-solubilized coal. An acid precipitation step was necessary to remove medium and/or microbial metabolites from solubilized coal to prevent overestimation of the extent of coal biosolubilization. Furthermore, the absorption spectra for different coal products varied, necessitating construction of standard curves for individual coals.
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  • 4
    ISSN: 1432-1432
    Keywords: Key words: Genome evolution — Adaptability — Somatic hypermutation — Affinity maturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Darwinian theory requires that mutations be produced in a nonanticipatory manner; it is nonetheless consistent to suggest that mutations that have repeatedly led to nonviable phenotypes would be introduced less frequently than others—if under appropriate genetic control. Immunoglobulins produced during infection acquire point mutations that are subsequently selected for improved binding to the eliciting antigen. We and others have speculated that an enhancement of mutability in the complementarity-determining regions (CDR; where mutations have a greater chance of being advantageous) and/or decrement of mutability in the framework regions (FR; where mutations are more likely to be lethal) may be accomplished by differential codon usage in concert with the known sequence specificity of the hypermutation mechanism. We have examined 115 nonproductively rearranged human Ig sequences. The mutation patterns in these unexpressed genes are unselected and therefore directly reflect inherent mutation biases. Using a χ2 test, we have shown that the number of mutations in the CDRs is significantly higher than the number of mutations found in the FRs, providing direct evidence for the hypothesis that mutations are preferentially targeted into the CDRs.
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  • 5
    ISSN: 1432-1432
    Keywords: Urate oxidase ; Drosophila pseudoobscura ; Drosophila melanogaster ; Nucleotide sequence ; Evolutionary comparison ; Gene regulation ; Malpighian tubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The urate oxidase (UO) transcription unit of Drosophila pseudoobscura was cloned, sequenced, and compared to the UO transcription unit from Drosophila melanogaster. In both species the UO coding region is divided into two exons of approximately equal size. The deduced D. pseudoobscura and D. melanogaster UO peptides have 346 and 352 amino acid residues, respectively. The nucleotide sequences of the D. pseudoobscura and D. melanogaster UO protein-coding regions are 82.2% identical whereas the deduced amino acid sequences are 87.6% identical with 42 amino acid changes, 33 of which occur in the first exon. Although the UO gene is expressed exclusively within the cells of the Malpighian tubules in both of these species, the temporal patterns of UO gene activity during development are markedly different. UO enzyme activity, UO protein, and UO mRNA are found in the third instar larva and adult of D. melanogaster but only in the adult stage of D. pseudoobscura. The intronic sequences and the extragenic 5′ and 3′ flanking regions of the D. pseudoobscura and D. melanogaster UO genes are highly divergent with the exception of eight small islands of conserved sequence along 772 by 5′ of the UO protein-coding region. These islands of conserved sequence are possible UO cis-acting regulatory elements as they reside along the 5′ flanking DNA of the D. melanogaster UO gene that is capable of conferring a wild-type D. melanogaster pattern of UO regulation on a UO-lacZ fusion gene.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 1050-1055 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Two rice α-amylase isozymes, AmylA and Amy3D, were produced by secretion from genetically engineered strains of Saccharomyces cerevisiae. They have distinct differences in enzymatic characteristics that can be related to the physiology of the germinating rice seed. The rice isozymes were purified with immunoaffinity chromatography. The pH optima for amy3D (pH optimum 5.5) and Amy1A (pH optimum 4.2) correlate with the pH of the endosperm tissue at the times in rice seedling development when these isozymes are produced. Amy3D showed 10–14 times higher reactivity to oligosaccharides than Amy1A. Amy1A, on the other hand, showed higher reactivity to soluble starch and starch granules than Amy3D. These results suggest that the isozyme Amy3D, which is expressed at an early stage of germination, produces sugars from soluble starch during the early stage of seed germination and that the isozyme Amy1A works to initiate hydrolysis of the starch granules.
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  • 7
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The acyl-coenzyme A-binding proteins (ACBPs) contain 26 highly conserved sequence positions. The majority of these have been mutated in the bovine protein, and their influence on the rate of two-state folding and unfolding has been measured. The results identify eight sequence positions, out of ...
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  • 8
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The human B lymphocyte activation antigen B7 provides regulatory signals for T lymphocytes as a consequence of binding to its ligands CD28 and CTLA-4. The cDNA for B7 has previously been isolated and predicted to encode a type I membrane protein. The predicted polypeptide has a secretory signal peptide followed by two contiguous Ig-like domains, a hydrophobic transmembrane region and a short cytoplasmic tail. Here we report the exon-intron genomic organization of human B7 and the chromosomal location. The gene has six exons that span approximately 32 kilobases of DNA. Exon 1 is not translated and the second exon contains the initiation ATG codon and encodes a predicted signal peptide. This gene structure is characteristic for several eukaryotic genes with tissue-specific expression. The third and fourth exons correspond to two Ig-like domains whereas the fifth and sixth exons encode respectively the trans-membrane portion and the cytoplasmic tail. This close relationship between exons and functional domains is a characteristic feature of genes of the Ig superfamily. Cell surface expression of the B7 gene product has previously been mapped to human chromosome 12 by antibody reactivity with the B7-specific monoclonal antibody BB-1. We here demonstrate that theB7 gene is located to theq21-qter region of chromosome 3 by DNA blot analysis of human × rodent somatic cell hybrids.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Semigroup forum 91 (1999), S. 671-675 
    ISSN: 1432-2137
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Lolium perenne L. cv. Regal and Pleasure) and tall fescue (Festuca arundinacea Schreb. cv. Martin and Rebel II). Seed yield components were measured to identify the components through which treatments affect yield. Treatments did not affect seed yield of Regal perennial ryegrass and Martin tall fescue in any crop year. Third-crop seed yield of Pleasure perennial ryegrass was 15% higher for the open-burn than for the flail-chop treatment; fourth-crop seed yield was 12% higher for the open-burn treatment than for either flail-chop or propane-burn. Across years, seed yields of Rebel II tall fescue for propane-burn and open-burn treatments were 8 and 12% greater, respectively, than for flail-chop. Where seed yields were affected, differences among treatments were through changes in seed number per unit area (seeds m-2), but seed weight was relatively stable. Treatments did not affect the number of fertile tillers in any cultivar. Variations in seed number were apparently due to treatment effects on the number of seeds set per tiller. We conclude that the need for postharvest residue burning in perennial ryegrass and tall fescue is cultivar specific. Mechanical residue removal is apparently as effective as open-field burning for maintaining high seed yields in some cultivars of these grasses.
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  • 10
    ISSN: 1432-2137
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Fetusca arundinacea Schreber) cultivars is needed to improve cultural practices for seed production. We tested factorial combinations of two post-harvest residue managements (flail-chopping or burning the stubble), two row spacing (0.3 and 0.6 m), three spring-applied N rates (100, 145, and 190 kg ha-1), and three N application timings (double-ridge, spikelet initiation, and equally split between the two) on seed yield components of turf-type cultivars Falcon, Rebel, and Bonanza, and the forage cultivar Fawn. Seed yields of all cultivars were closely correlated with the number of seeds produced per unit area (m2), but yields were weakly and negatively correlated with weight per seed. Compared with flail-chopping, burning the residue increased the number of fertile tillers, floret sites, and seeds per unit area in all cultivars. The number of seeds per unit area was not affected by row spacing in Fawn and Bonanza, but was increased at 0.3-m row spacing in Falcon when the residue was burned, and was greater at 0.6-m in Rebel when the residue was flail-chopped. Increasing N rate above 100 kg ha-1 decreased floret site utilization and seeds per unit area in Fawn, but did not affect these components in Falcon and Rebel. Seeds per unit area in Bonanza was the greatest at 145 kg N ha-1. For all cultivars, N application timing had little effect on seeds per unit area. We conclude that management practices for the turf-type cultivars should focus on maintaining a high number of fertile tillers throughout the stand life by burning post-harvest residue. Using narrower row spacings for early- and mid-maturing turf-type cultivars also could increase the number of fertile tillers per unit area when the post-harvest residue is burned. The optimum spring-applied N rate for seed production is apparently greater for the late-maturing turf types than for forage cultivars.
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