Publication Date:
1995-08-04
Description:
The p27 mammalian cell cycle protein is an inhibitor of cyclin-dependent kinases. Both in vivo and in vitro, p27 was found to be degraded by the ubiquitin-proteasome pathway. The human ubiquitin-conjugating enzymes Ubc2 and Ubc3 were specifically involved in the ubiquitination of p27. Compared with proliferating cells, quiescent cells exhibited a smaller amount of p27 ubiquitinating activity, which accounted for the marked increase of p27 half-life measured in these cells. Thus, the abundance of p27 in cells is regulated by degradation. The specific proteolysis of p27 may represent a mechanism for regulating the activity of cyclin-dependent kinases.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pagano, M -- Tam, S W -- Theodoras, A M -- Beer-Romero, P -- Del Sal, G -- Chau, V -- Yew, P R -- Draetta, G F -- Rolfe, M -- New York, N.Y. -- Science. 1995 Aug 4;269(5224):682-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Mitotix Inc., Cambridge, MA 02139, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7624798" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphate/metabolism
;
Anaphase-Promoting Complex-Cyclosome
;
Animals
;
*Cell Cycle Proteins
;
Cell Line
;
Cyclin-Dependent Kinase Inhibitor p27
;
Cyclin-Dependent Kinases/*antagonists & inhibitors
;
Cysteine Endopeptidases/*metabolism
;
Electroporation
;
Enzyme Inhibitors/metabolism
;
Humans
;
Kinetics
;
Leupeptins/pharmacology
;
Ligases/metabolism
;
Mice
;
Microtubule-Associated Proteins/*metabolism
;
Multienzyme Complexes/*metabolism
;
Proteasome Endopeptidase Complex
;
Rabbits
;
Recombinant Proteins/metabolism
;
Succinates/pharmacology
;
Tumor Cells, Cultured
;
*Tumor Suppressor Proteins
;
Ubiquitin-Conjugating Enzymes
;
*Ubiquitin-Protein Ligase Complexes
;
Ubiquitin-Protein Ligases
;
Ubiquitins/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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