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  • Blackwell Publishing Ltd  (105)
  • Wiley-Blackwell  (72)
  • 1995-1999  (48)
  • 1990-1994  (103)
  • 1960-1964  (26)
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  • 1
    Electronic Resource
    Electronic Resource
    Production and characterization of a plant α-hydroxynitrile lyase in Escherichia coli (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 53 (1997), S. 332-338 
    Details
    ISSN: 0006-3592
    Keywords: α-hydroxynitrile lyase ; cassava ; cyanogenesis ; cyanohydrin ; Escherichia coli expression vector ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The coding sequence of the cyanogenic α-hydroxynitrile lyase gene of Manihot esculenta Crantz (cassava) was cloned in the plasmid vector pMal-c2 and expressed in Escherichia coli strain JM105. DNA sequencing showed that the recombinant plasmid contained the same sequence as the cDNA clone pHNL10. Peptide sequencing of the recombinant protein showed that the N-terminus was heterogeneous, with either four or six additional amino acid residues compared with the native protein. Circular dichroism spectra indicated similar secondary structure contents for both proteins. Enzyme assays showed that specific activity of native and recombinant proteins were 0.24 and 0.26 mmol CN-/mg/min, respectively; that both proteins had optimal activity at 40°C and pH 5.5; and that both proteins were inhibited by the serine protease inhibitor phenyl-methane sulfonyl flouride (PMSF). Isoelectric focusing of native and recombinant protein revealed multiple isoforms for both proteins; the recombinant protein had a more basic mean isoelectric point (pl) (5.1) than the native protein (4.5). © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 332-338, 1997.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructure of Mitosis and Cytokinesis In the Colorless Flagellate Katablepharis Ovalis Skuja (1993)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 40 (1993), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Mitosis and cytokinesis in Katablepharis ovalis, a colorless flagellate, was investigated. Two new flagella are produced prior to prophase, resulting in a motile quadriflagellate cell during mitosis. the inner array of microtubules of the feeding apparatus disappears before prophase begins. the nuclear envelope disperses during prophase, apparently being converted into rough endoplasmic reticulum. the chromatin condenses and the nucleolus disperses with spindle microtubules appearing oriented perpendicular to the longitudinal axis of the cell. At metaphase, the chromatin is condensed as a single disc-shaped mass and rough endoplasmic reticulum flanks the chromatin mass on each side. Groups of spindle microtubules pass through tunnels in the rough endoplasmic reticulum and through electron-translucent areas of the chromatin. the spindle microtubules end at a number of minipoles in the cytoplasm. Vesicles, ribosomes, mitochondria and endoplasmic reticulum migrate among the spindle microtubules. There is no polar body or any electrondense area associated with the spindle poles. the basal bodies of the flagella remain attached to the axonemes and do not participate in mitosis. In anaphase, the chromatin separates and migrates to the poles. During telophase, the nuclear envelope reforms from the rough endoplasmic reticulum and the nucleoli reappear. the spindle microtubules are persistent during telophase. Cytokinesis occurs by longitudinal fission, starting at the anterior end and progressing posteriorly. Cytokinesis may be driven by elongation of the spindle microtubules since there is no visible structure associated with the furrowing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1993.tb04931.x
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  • 3
    Electronic Resource
    Electronic Resource
    The retention of nitrate in acid soils from the tropics (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 6 (1990), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. In most soils of temperate regions nitrate is not held on soil surfaces and moves freely in solution. But when soils carry positive charges, nitrate is held as an exchangeable anion. As a result, leaching of nitrate is delayed relative to the movement of water. The delay can be predicted provided the anion exchange capacity (AEC) can be measured and the concentration of counter-anions is known. For soils with variable charge, the AEC varies with both pH and ionic strength, and the effective AEC should be determined under conditions similar to those in soil solution. A simple leaching method is described which satisfies this requirement. Delays in the leaching of nitrate measured in columns of repacked soil were strongly related to the AEC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.1990.tb00805.x
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  • 4
    Electronic Resource
    Electronic Resource
    Release of bromide following rewetting of a naturally drained acid gully mire (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 12 (1996), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Currently there is growing interest in the restoration of drained peatlands in Britain. In order to investigate some of the effects of changes in land management practices on the biogeochemistry of peatlands, a field experiment was designed to manipulate the hydrological conditions in a naturally drained acid gully mire in Mid-Wales. We report preliminary results of the effects of experimentally rewetting the mire on the hydrochemistry of bromide in the peat-water. Results show that rewetting had a dramatic effect on the concentrations of bromide, which increased substantially. Peak values approached 1 mg/dm3 in some samples following rewetting, compared with typical values 〈 0.05 mg/dm3 under the drained conditions. Bromide was positively and significantly correlated with Dissolved Organic Carbon (DOC) in the peat-water, which suggests that Br- is derived from breakdown of organic matter in the reducing conditions following rewetting. The bromide mobilized by rewetting may be leached out of the system and/or re-assimilated by the wetland vegetation. Further monitoring is needed to determine whether the observed hydrochemical response of bromide to rewetting of the mire has any longer-term effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.1996.tb00960.x
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  • 5
    Electronic Resource
    Electronic Resource
    Temporal trends in bromide release following rewetting of a naturally drained gully mire (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 14 (1998), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Recently, there has been interest in the occurrence of bromide (Br-) in natural waters since it has been demonstrated that Br-, in association with humic substances in raw waters, is readily incorporated into haloacetic acids in the form of organically bound bromine (Br) during water chlorination. We report results of the effects of experimentally rewetting a naturally drained gully mire on the hydrochemistry of Br-, iron (Fe) and dissolved organic carbon (DOC) in the peat water. Results obtained over a three year period showed that rewetting substantially increased the concentrations of these solutes in the pore water, with peak values of 1 mg dm-3 (Br-), 〉 60 mg dm-3 (Fe) and 〉 300 mg dm-3 (DOC) detected in some samples after rewetting, compared with typical values 〈 0.05 mg dm-3 (Br-), 〈 1 mg dm-3 (Fe) and 〈 15 mg dm-3 (DOC) under the drained conditions. Bromide, Fe and DOC release were highly seasonal, with the largest concentrations observed in late-summer to autumn. However, whereas seasonal peak concentrations of Fe and DOC have since remained at these higher levels, seasonal peak concentrations of Br- were progressively attenuated over time, suggesting the latter phenomenon is a flush effect, with no longer-term consequences for water quality.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.1998.tb00161.x
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  • 6
    Electronic Resource
    Electronic Resource
    Effects of clearfelling on microbial biomass phosphorus in the Oh horizon of an afforested podzol in Mid-Wales (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 7 (1991), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Microbial biomass C and P were monitored in the Oh horizon of an afforested podzol in Mid-Wales before and after clearfelling. Measurements over a 2-year period indicated that soil moisture and temperature control the seasonal fluctuations in biomass C in this soil. Clearfelling significantly increased biomass P in the Oh horizon. This was probably caused in part by increased fluxes of organic and inorganic phosphorus from the felling debris and litter horizons of the forest floor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.1991.tb00872.x
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  • 7
    Electronic Resource
    Electronic Resource
    The influence of land management on concentrations of dissolved organic carbon and its effects on the mobilization of aluminium and iron in podzol soils in Mid-Wales (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 6 (1990), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. The aluminium (Al), iron (Fe) and Dissolved Organic Carbon (DOC) contents of the soil solution were monitored in two upland grassland and afforested podzol soils in Mid-Wales. Al organo-metallic complexes predominated in the O horizon leachates of the grassland soil, whereas inorganic monomeric Al forms dominated in the lower mineral horizons. Dissolved organic matter determines the chemistry, solubility, and transport of Al and Fe in the O horizon, and these are under strong biological control. The distributions of organic-Al, Fe and DOC within the soil profile were consistent with traditional podzolization theory. Observed increases in the molar ratios of Al:DOC in solution in the lower soil horizons may be responsible for the small solubility of Al organo-metallic complexes in those horizons. Afforestation increased the concentrations of organic-Al and Fe in the soil solution as compared with the concentrations observed for the grassland soil. Clearcutting further significantly mobilized Al and Fe from the upper soil horizon, primarily by increasing the DOC concentration in the soil water.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.1990.tb00823.x
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  • 8
    Electronic Resource
    Electronic Resource
    THE GRASSLANDS OF EAST ANGLIA (1963)
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 18 (1963), S. 0 
    Details
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Because in the past the main emphasis was placed on arable farming, grassland husbandry has played a minor role in East Anglia. Now the increased efficiency exercised in the arable farming of the area is being applied to the grasslands. The main trends are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2494.1963.tb00357.x
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  • 9
    Electronic Resource
    Electronic Resource
    THE ROLE OF COCKSFOOT (DACTYLIS GLOMERATA) IN GRASSLAND HUSBANDRY IN BRITAIN (1962)
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 17 (1962), S. 0 
    Details
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Opinions vary among research workers, grassland husbandry advisers and farmers alike as to the value of cocksfoot (Dactylis glomerata) as a herbage plant both with regard to its quality for the feeding of livestock and its ability to build up soil organic matter reserves. An attempt is made to assess the value of this grass in agricultural practice, to place in proper perspective its use over the past few decades, and to indicate where its greatest potential lies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2494.1962.tb00301.x
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  • 10
    Electronic Resource
    Electronic Resource
    COMPARISONS OF GRASS VARIETIES FOR SURFACE SEEDING UPLAND PASTURE TYPES (1962)
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 17 (1962), S. 0 
    Details
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A study was made of the agronomic behaviour of 18 grass varieties, surface sown, after burning an upland heath community dominated by Calluna vulgaris. Complete exposure of the soil surface, the absence of competition and favourable moisture conditions provided excellent conditions for establishment. Despite this, sward development was slower than at any other site studied, and 18 months elapsed before satisfactory sward densities, and consequently a reasonable level of production, were achieved. In contrast, white clover developed vigorously from the outset. The red fescues, cocksfoots and S170 were the most promising, while the performance of most varieties was affected by one or more hazards such as frost-heaving and summer drought. The latter aggravated the tendency of some of the varieties to produce a profusion of flowering stems and, with few exceptions, considerably reduced the colour and vigour of the herbage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2494.1962.tb00272.x
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