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  • Daucus (somatic embryogenesis)  (1)
  • Disturbance  (1)
  • 1995-1999  (1)
  • 1990-1994  (1)
  • 1960-1964
  • 1955-1959
  • 1870-1879
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  • 1995-1999  (1)
  • 1990-1994  (1)
  • 1960-1964
  • 1955-1959
  • 1870-1879
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  • 1
    ISSN: 1432-2048
    Keywords: Daucus (somatic embryogenesis) ; Embryogenesis (somatic) ; Peroxidase, cationic (isoenzymes) ; Protein glycosylation ; Glycoprotein secretion ; Somatic embryogenesis ; Tunicamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryogenesis of carrot (Daucus carota L.) is inhibited by the glycosylation inhibitor tunicamycin. This inhibition is reversible by the addition of correctly glycosylated glycoproteins which have been secreted into the culture medium. To identify the proteins responsible for complementation, glycoproteins present in the medium of embryo cultures were purified and tested for their activity in the tunicamycin inhibition/ complementation assay. A 38-kDa glycoprotein was purified that could restore embryogenesis to more than 50% of that in untreated controls. This 38-kDa glycoprotein was identified as a heme-containing peroxidase on the basis of its A405/A280 ratio (Reinheit Zahl or RZ) and enzyme activity. The 38-kDa peroxidase consisted of four different cationic isoenzymes of which only one or possibly two appeared active in the complementation assay. The cationic peroxidase isoenzymes from the carrot medium could be effectively replaced by cationic horseradish peroxidases which depended on their catalytic properties for their ability to restore tunicamycin-inhibited somatic embryogenesis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant ecology 145 (1999), S. 83-90 
    ISSN: 1573-5052
    Keywords: Dispersal ; Disturbance ; Granivory ; Germinable seed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sources of seed for seedbank build-up in an acidic grassland were identified from analysis of differences in seedbank build-up over one year between plots where the input of rabbit pellets to ‘seed-free’ soil had either been left or removed. In parallel, the flux of seed arriving in rabbit pellets was monitored. Pellet seed content and total seed input were highest in late summer/early autumn and again in the spring. The seed content of the pellets was dominated by a small number of species: Sagina apetala, Senecio jacobaea, Urtica dioica and Veronica arvensis. Smaller seeded species were more likely to be present as germinable seed in the pellets. Seedbank build-up as a result of wind, splash or adhesive dispersal totalled 547 seedsm2. The additional effect of allowing seed input in pellets was 267 seedsm2, though this increase was not significant. The total increase in seedbank over one year was equivalent to 15–20% of the seedbank present in undisturbed soil. Eight species showed a significant build-up of seed in the seedbank over one year as a result of all means of dispersal, but only Myosotis discolor showed a significantly higher soil germinable seed content in the plots where pellets had been allowed to remain in situ. The build-up of seed in the seedbank is contrasted with the build-up of vegetation on disturbed areas within the same system.
    Type of Medium: Electronic Resource
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