ISSN:
1573-9686
Schlagwort(e):
Microcirculation
;
Arterioles
;
Smooth muscle
;
Muscle mechanics
;
Vessel reactivity
;
Vascular resistance
;
Microsurgery
;
Microperfusion
;
Hamster
;
Cheek pouch
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
,
Technik allgemein
Notizen:
Abstract In vivo studies on frog mesenteric arterioles (4) indicate that segmental differences in the response of microvessels to physical and chemical stimuli can be explained simply in terms of the length-tension characteristics of vascular smooth muscle at diferent points along the vascular tree. Studies on single, isolated arterioles in vitro were initiated to examine more closely the validity of this explanation for regional response differences. This paper reports some of the results. First-, second-, and third-order arterioles (18–60 μm i.d.) were dissected from hamster cheek pouches. The vessels were cannulated with a modified Burg microperfusion system, and their mechanical properties studied using the methods described by Duling and Gore (3). Vessels were activated in four stages with K+ and norepinephrine. During activation, transmural pressures were adjusted to minimize vascular smooth-muscle shortening. Active pressure-diameter curves were recorded while adjusting transmural pressure through the range 5 to 400 cm H2O in 5–25 cm steps. Vessel dimensions were measured with a videomicrometer. Passive curves were obtained after equilibration overnight in Ca2+-free medium. The vessels were then fixed and prepared for histologic sectioning, and measurements of vessel-wall composition were made. The Laplace relationship was used to construct length-tension diagrams, and the histologic data were used to normalize the dimensional data to smooth-muscle lengths. Maximum active tension of second-order arterioles (1,170 dynes/cm) was two times previous values reported by Gore et al. (5). This was due presumably to refinements in techniques and dissection procedures. Maximum active stress averaged 3.9×10+6 dynes/cm2 for second-order arterioles. This number is identical to data obtained from hog carotid strips by Dillon et al. (2).
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02363920
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