ISSN:
1573-5028
Keywords:
Agrobacterium tumefaciens
;
hypocotyls
;
Nicotiana alata
;
regeneration
;
self-incompatibility
;
transformation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A transformation and regeneration system has been developed for Nicotiana alata, a plant which is being intensively studied as a model of gametophytic self-incompatibility. Plantlets can be regenerated efficiently from seedling hypocotyls. Kanamycin-resistant, transformed plants have been obtained by cocultivation of regenerating hypocotyls with Agrobacterium tumefaciens strain LBA4404 containing a binary vector. The transformation frequency was low with 〈1% of tissue explants regenerating transformed plants. The transformed plants contained from one to three copies of the introduced DNA. In most cases, the kanamycin resistance phenotype was transmitted to the offspring as a normal Mendelian factor. In one unusual case, none of the offspring inherited the kanamycin resistance of the transformed maternal parent. This plant may have been chimeric or the kanamycin resistance gene may have been inactivated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00016514
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