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  • Nicotiana alata  (2)
  • coconut palm  (2)
  • Springer  (4)
  • Oxford University Press
  • Wiley
  • 1995-1999
  • 1990-1994  (3)
  • 1985-1989  (1)
  • 1980-1984
  • 1970-1974
Sammlung
Verlag/Herausgeber
  • Springer  (4)
  • Oxford University Press
  • Wiley
Erscheinungszeitraum
  • 1995-1999
  • 1990-1994  (3)
  • 1985-1989  (1)
  • 1980-1984
  • 1970-1974
Jahr
  • 1
    ISSN: 1573-5028
    Schlagwort(e): glycoproteins ; Nicotiana alata ; pollination ; self-incompatibility ; S-locus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A summary of recent work on molecular aspects of self-incompatibility in Nicotiana alata is presented. The amino acid sequences of style proteins corresponding to different S-alleles of N. alata have a high level of homology in some regions and are variable in other regions. The regions of homology include N-terminal sequences as well as most of the glycosylation sites and cysteine residues. The glycosyl substituents may consist of a number of ‘glycoforms’. The isolated style S-glycoproteins inhibit in vitro growth of pollen tubes. The S-glycoproteins tested inhibited the growth of pollen of several S-genotypes, and there was some specificity in the interaction. Heat treatment of the isolated S-glycoproteins dramatically increased their activity as inhibitors of pollen tube growth, although the specificity in the interaction was lost. The nature of the S-allele products in pollen is not yet established.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Plant molecular biology 14 (1990), S. 815-824 
    ISSN: 1573-5028
    Schlagwort(e): Agrobacterium tumefaciens ; hypocotyls ; Nicotiana alata ; regeneration ; self-incompatibility ; transformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A transformation and regeneration system has been developed for Nicotiana alata, a plant which is being intensively studied as a model of gametophytic self-incompatibility. Plantlets can be regenerated efficiently from seedling hypocotyls. Kanamycin-resistant, transformed plants have been obtained by cocultivation of regenerating hypocotyls with Agrobacterium tumefaciens strain LBA4404 containing a binary vector. The transformation frequency was low with 〈1% of tissue explants regenerating transformed plants. The transformed plants contained from one to three copies of the introduced DNA. In most cases, the kanamycin resistance phenotype was transmitted to the offspring as a normal Mendelian factor. In one unusual case, none of the offspring inherited the kanamycin resistance of the transformed maternal parent. This plant may have been chimeric or the kanamycin resistance gene may have been inactivated.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell, tissue and organ culture 33 (1993), S. 157-162 
    ISSN: 1573-5044
    Schlagwort(e): activated charcoal ; BAP adsorption ; 2,4-D adsorption ; coconut palm ; tissue culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The rate of adsorption of 6-benzylaminopurine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D) by activated charcoal (AC) from liquid and semi-solid tissue culture media was determined using 8-[14C]-BAP and 2-[14C]-2′,4′-D. For both liquid and semi-solid media an essentially constant ratio of free BAP: AC-bound BAP was reached between 5 and 10 days after preparation of the media. Pronounced increases in the level of available 2,4-D in the medium occurred, when either the initial BAP or 2,4-D level or both were raised to 5 × 10−4 M. When 2,4-D was used at 5 × 10−4 M the medium component sucrose (40 g l−1) caused a pronounced increase in available 2,4-D. Conversely the inclusion of the combined macro- and micronutrients markedly reduced the availability of free 2,4-D in the medium.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell, tissue and organ culture 20 (1990), S. 165-172 
    ISSN: 1573-5044
    Schlagwort(e): activated charcoal ; 2,4-D adsorption ; coconut palm ; tissue culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The rate of adsorption of 2,4-dichlorophenoxyacetic acid (2,4-D) by activated charcoal (AC) from liquid and semi-solid tissue culture media was determined using 2-[14C]-2′,4′-D. In liquid medium 99.5% of the added 2,4-D (10-4 M) was adsorbed by AC (2.5 gl-1) within 5 days of preparation of the medium. Higher 2,4-D levels of reduced AC concentrations increased the level of available 2,4-D in the medium and extended the period necessary for the level of 2,4-D in the medium to become stabilized. In semi-solid medium the rate of adsorption of 2,4-D by AC was considerably reduced. A stable ratio of gel/2,4-D:AC/2,4-D was only reached after 10 to 20 days, depending on the 2,4-D concentration used. Low pH levels and maintenance of the medium at higher temperatures (20–30°C) accelerated the adsorption of 2,4-D by AC. In vitro tissue cultures of coconut palm showed marked differences in their growth response according to the age of the medium used and the associated variations in 2,4-D concentrations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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