Publication Date:
2003-04-01
Description:
For the first time, all stages required for the micropropagation of sugar maple (Acer saccharum Marsh.) have been successfully completed. Buds of more than 200 seedlings of two-year-old sugar maple trees were stripped of their scales and cultured on media containing Murashige and Skoog salts with Bourgin and Nitsch organic elements, all diluted to 33%, with or without growth regulator. Survival rate after induction was higher in media without growth regulator. Stem formation and multiplication succeeded in media of the same composition but containing 0.01 or 0.025 mg·L1 thidiazuron, as well as 6-(γ, γ-dimethylallylamino)purine, 6-(γ, γ-dimethylallylamino)purine riboside, or phenylacetic acid (PAA). Stem elongation took place in media with 0.3 mg·L1 PAA. Root induction was achieved in media containing 0.3 mg·L1 PAA with naphthaleneacetic acid. A few shoots rooted when transferred into vermiculite. Although the overall rate of success is still low, rooted shoots were produced by bud multiplication.
Print ISSN:
0045-5067
Electronic ISSN:
1208-6037
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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