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  • 2000-2004  (2)
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  • 1
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In order to identify chromosomes involved in resistance to Fusarium head blight, a set of 21 substitution lines of Triticum macha (resistant) chromosomes into ‘Hobbit’'sib’(susceptible) were evaluated in trials over 2 years. For the first year's trial, all plants were inoculated on the same day with a conidial suspension of F. culmorum. For the second trial, individual plants were inoculated precisely at mid anthesis of each plant over a period of 2 weeks. The disease level was assessed by visual scoring, relative ear weight and F. culmorumn-specfic quantitative polymerase chain reaction. The results showed that T. macha chromosomes 1B, 4A and 7A conferred good overall resistance, suggesting that they carry important genes for resistance. In two additional trials, T. macha and ‘Hobbit’'sib’ were evaluated for resistance to brown foot rot. The results showed that T. macha was more susceptible than ‘Hobbit’‘sib’, indicating that stem base disease response is not correlated with head blight resistance in these cultivars.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 49 (2000), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A collection of group II Fusarium graminearum isolates obtained from maize, wheat and rice from different locations in Nepal were identified using a combination of morphological and molecular criteria. The variation within this collection was analysed using RAPD markers, intergenic spacer (IGS) RFLP and PCR polymorphisms. The isolates were divided into two groups, A and B, by RAPD analysis. Isolates in group A yielded four different PCR polymorphic markers, but all the isolates in group B yielded a single polymorphic marker. The IGS RFLP analysis was consistent with division of the isolates into two groups. Isolates from wheat and rice were more frequently placed in group A, with isolates from maize more evenly distributed between the groups. Results indicate that host preference might be a factor in the division of isolates, although the year of isolation may also have had an influence. No geographical factors or agricultural practices could be identified to account for the observed variation.
    Type of Medium: Electronic Resource
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