ISSN:
1432-203X
Keywords:
Key words Friable embryogenic callus
;
Regeneration
;
Rhizome
;
Alstroemeria
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Stem segments of seedlings from two Alstroemeria breeding lines, cultured on media supplemented with 4 mg/l 2,4-dichlorophenoxyacetic acid and 0.5–1.0 mg/l 6-benzylaminopurine (BA), initiated soft callus, which became compact after subculture on a medium with only 0.5 mg/l BA. Friable embryogenic calli were initiated from compact callus on a medium supplemented with 10 mg/l picloram. Proembryos developed from friable embryogenic calli via embryos into plants after subculture on medium supplemented with 0.1 mg/l BA. The proembryos formed friable embryogenic calli again after culture on medium supplemented with 10 mg/l picloram. The total time needed to regenerate a complete plantlet from friable callus was approximately 6 months. This system for the production of embryogenic material is considered to have valuable applications for genetic transformation in Alstroemeria.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050768
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