Publikationsdatum:
2004-01-24
Beschreibung:
Arabidopsis thaliana De-etiolated-1 (AtDET1) is a highly conserved protein, with orthologs in vertebrate and invertebrate organisms. AtDET1 negatively regulates photomorphogenesis, but its biochemical mechanism and function in other species are unknown. We report that human DET1 (hDET1) promotes ubiquitination and degradation of the proto-oncogenic transcription factor c-Jun by assembling a multisubunit ubiquitin ligase containing DNA Damage Binding Protein-1 (DDB1), cullin 4A (CUL4A), Regulator of Cullins-1 (ROC1), and constitutively photomorphogenic-1. Ablation of any subunit by RNA interference stabilized c-Jun and increased c-Jun-activated transcription. These findings characterize a c-Jun ubiquitin ligase and define a specific function for hDET1 in mammalian cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wertz, Ingrid E -- O'Rourke, Karen M -- Zhang, Zemin -- Dornan, David -- Arnott, David -- Deshaies, Raymond J -- Dixit, Vishva M -- GM065997/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Feb 27;303(5662):1371-4. Epub 2004 Jan 22.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Oncology, Genentech, Inc., South San Francisco, CA 94080, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14739464" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Amino Acid Motifs
;
Amino Acid Sequence
;
Carrier Proteins/chemistry/genetics/*metabolism
;
Cell Line
;
Cloning, Molecular
;
Cullin Proteins/genetics/*metabolism
;
DNA-Binding Proteins/metabolism
;
Genes, jun
;
Humans
;
Molecular Sequence Data
;
Nuclear Proteins/chemistry/genetics/metabolism
;
Protein Binding
;
Proteomics
;
Proto-Oncogene Proteins c-jun/*metabolism
;
RNA, Messenger/genetics/metabolism
;
RNA, Small Interfering/metabolism
;
Transfection
;
Ubiquitin/metabolism
;
Ubiquitin-Protein Ligases/chemistry/*metabolism
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
Permalink
