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  • Hevea brasiliensis  (1)
  • doubled haploids  (1)
  • 2000-2004  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Microspore-derived embryo induction from cultured anthers of wheat (2000)
    Springer
    Plant cell, tissue and organ culture 60 (2000), S. 235-238 
    Details
    ISSN: 1573-5044
    Keywords: anther culture ; doubled haploids ; microspore-derived embryos ; phenylacetic acid ; Poaceae ; Triticum aestivum L. cv. Pavon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microspore-derived embryo (MDE) induction was evaluated following the culture of wheat (Triticum aestivum L.) anthers in a basal medium with a range of supplements. Several supplements were evaluated, including a commercial bovine haemoglobin solution (Erythrogen™), Ficoll and the co-polymer surfactant Pluronic F-68, but these did not enhance MDE induction. However, phenylacetic acid, replacing both 2,4-dichlorophenoxyacetic acid and kinetin, increased MDE induction by a factor of 1.6 (p〈0.05), producing 257±29 (mean±s.e., n=3) MDEs per 100 anthers cultured. Twenty-four plants regenerated from MDEs induced via anther culture were used for ploidy determinations; 33% were sterile haploids, while the remainder were fertile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006400915551
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  • 2
    Electronic Resource
    Electronic Resource
    Plant regeneration from embryogenic cell suspension-derived protoplasts of rubber (2000)
    Springer
    Plant cell, tissue and organ culture 61 (2000), S. 81-85 
    Details
    ISSN: 1573-5044
    Keywords: cell suspensions ; embryogenesis ; Hevea brasiliensis ; plant regeneration ; protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Embryogenic cell suspensions of rubber derived from immature inflorescences and inner integuments of immature fruits released 3.1 ± 0.2 × 107 protoplasts g-1 f. wt. (mean ± s.e.m, n = 10) and 3.2 ± 0.2 × 107 protoplasts g-1 f. wt., with mean viabilities of 83 ± 2% and 77 ± 8%, respectively. Sustained mitotic division was observed only when protoplasts were cultured in KPR liquid medium on nitrocellulose membranes overlying the same semi-solid medium containing Lolium multiflorum nurse cells. Protoplast-derived cell colonies were produced within 2 months of culture. Protoplast-derived cell colonies proliferated, upon subculture to MS-based regeneration medium, with 40% of the protoplast-derived calli developing somatic embryos. The latter germinated into plants on the same medium after 3 months of culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006494404224
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    Paper (German National Licenses)
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