ISSN:
1572-8773
Keywords:
canine
;
concanavalin A
;
ferritin-binding protein
;
ferritin iron
;
G subunit
;
serum ferritin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A sandwich enzyme-linked immunosorbent assay using H-subunit-rich canine heart ferritin as a standard has been developed for measuring canine serum ferritin which is H-subunit-rich. Serum ferritin concentrations in 51 normal dogs ranged from 143 to 1766 ng ml−1, with a mean value of 479±286 (SD) ng ml−1. Serum ferritin iron concentrations as determined by an immunoprecipitation technique ranged from 30.4 to 115.9 ng ml−1 in 15 normal dogs with serum ferritin protein levels of 298 to 959 ng ml−1. There was a significant linear correlation between the serum ferritin iron and protein levels (r=0.9441, P〈0.001), and the mean iron/protein ratio of serum ferritin was 0.112±0.017. When canine sera were incubated with concanavalin A-Sepharose 4B, we observed the apparent binding of serum ferritin to concanavalin A. However, ferritin obtained by heat-treating the sera at pH 4.8 to remove the ferritin-binding proteins did not bind to the lectin. These results suggest that canine serum ferritin contains a considerable amount of iron but no concanavalin A-binding G subunit present in human serum ferritin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009276323707
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