Publication Date:
2002-11-26
Description:
The untranslated roX1 and roX2 RNAs are components of the Drosophila male-specific lethal (MSL) complex, which modifies histones to up-regulate transcription of the male X chromosome. roX genes are normally located on the X chromosome, and roX transgenes can misdirect the dosage compensation machinery to spread locally on other chromosomes. Here we define MSL protein abundance as a determinant of whether the MSL complex will spread in cis from an autosomal roX transgene. The number of expressed roX genes in a nucleus was inversely correlated with spreading from roX transgenes. We suggest a model in which MSL proteins assemble into active complexes by binding nascent roX transcripts. When MSL protein/roX RNA ratios are high, assembly will be efficient, and complexes may be completed while still tethered to the DNA template. We propose that this local production of MSL complexes determines the extent of spreading into flanking chromatin.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Park, Yongkyu -- Kelley, Richard L -- Oh, Hyangyee -- Kuroda, Mitzi I -- Meller, Victoria H -- GM45744/GM/NIGMS NIH HHS/ -- GM58427/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2002 Nov 22;298(5598):1620-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12446910" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Chromatin/*metabolism
;
Chromosomes/metabolism
;
DNA, Complementary
;
DNA-Binding Proteins
;
*Dosage Compensation, Genetic
;
Drosophila/*genetics/metabolism
;
*Drosophila Proteins
;
Gene Expression Regulation
;
Mutation
;
Nuclear Proteins/genetics/*metabolism
;
RNA, Messenger/*genetics/metabolism
;
RNA, Untranslated/*genetics/metabolism
;
Transcription Factors/genetics/*metabolism
;
Transcription, Genetic
;
Transgenes
;
X Chromosome/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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