ISSN:
1432-0983
Keywords:
Key wordsKluyveromyces lactis
;
Mitochondrial ribosomal protein
;
ρo-lethality
;
L23
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The Kluyveromyces lactis nuclear gene, MRP-L23, encodes a polypeptide of 155 amino acids that shares 70% and 43% identity to the ribosomal proteins L23 and L13 of Saccharomyces cerevisiae and Escherichia coli. The deduced protein, designated KlL23, is a likely component of the large subunit of mitochondrial ribosomes as it can complement the respiratory deficient phenotype of a S. cerevisiae mrp-L23 mutant. As in S. cerevisiae, KlMRP-L23 is essential for respiratory growth of K. lactis because disruption of the gene in a “petite-positive” strain carrying a ρo-lethality suppressor atp mutation rendered cells unable to grow on a non-fermentable carbon source. However, in contrast to S. cerevisiae, disruption of MRP-L23 in wild type K. lactis is lethal. Meiotic segregants of K. lactis with a disrupted MRP-L23 allele form microcolonies with cell numbers varying from 32 to 300. These data clearly indicate an essential role of mitochondrial protein synthesis for viability of the petite-negative yeast K. lactis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050014
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