Publication Date:
2000-02-05
Description:
Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying pheromone signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 of the most highly regulated genes, was constructed from 46 diverse experimental conditions. Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple mitogen-activated protein kinase (MAPK) pathways. Analysis of the profiles specified by two different MAPKs-Fus3p and Kss1p-revealed functional overlap of the filamentous growth and mating responses. Global transcript analysis reflects biological responses associated with the activation and perturbation of signal transduction pathways.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roberts, C J -- Nelson, B -- Marton, M J -- Stoughton, R -- Meyer, M R -- Bennett, H A -- He, Y D -- Dai, H -- Walker, W L -- Hughes, T R -- Tyers, M -- Boone, C -- Friend, S H -- New York, N.Y. -- Science. 2000 Feb 4;287(5454):873-80.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Rosetta Inpharmatics, 12040 115th Avenue Northeast, Kirkland, WA 98034, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10657304" target="_blank"〉PubMed〈/a〉
Keywords:
*Cell Cycle Proteins
;
Cyclin-Dependent Kinase Inhibitor Proteins
;
Fungal Proteins/genetics/metabolism/physiology
;
G1 Phase
;
*Gene Expression Profiling
;
*Gene Expression Regulation, Fungal
;
Genome, Fungal
;
Lipoproteins/pharmacology/physiology
;
*MAP Kinase Signaling System
;
Mitogen-Activated Protein Kinases/metabolism
;
Multigene Family
;
Oligonucleotide Array Sequence Analysis
;
Peptides/pharmacology/physiology
;
Pheromones
;
Protein Kinase C/metabolism
;
*Repressor Proteins
;
Saccharomyces cerevisiae/cytology/*genetics/growth & development/physiology
;
*Saccharomyces cerevisiae Proteins
;
Transcription Factors/metabolism
;
Transcriptional Activation
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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