ISSN:
1432-1017
Keywords:
H-NMR
;
photo-CIDNP
;
PSTI Met-enkephalin
;
β-Endorphins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract The photo-CIDNP technique is capable of giving information, not only on the degree of exposure of aromatic residues in proteins and on the correlation time of aromatic side chains, but also on the fine structure of β-CH2 multiplets, whose J-pattern directly reflects the side chain orientation about X 1. Moreove, the resonance filtering achieved in the difference spectra allows selective measurement of line broadenings in binding processes, which are directly related to the association constants (K a ). In this communication, data are reported concerning the exposure, mobility and orientation of the tyrosyl side chains in bovine PSTI at different pH values. The immobilization of tyrosyl residues in Met-enkephalin, human, and camel β-endorphin in the presence of lipid micelles is also investigated; the extent of interaction of the three peptides with C12PN is discussed. The binding efficiency is considerably lower for the shorter peptide; of the two endorphins, the camel hormone exhibits a higher affinity for the micelles than the human analog.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00257397
Permalink