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  • 1
    Electronic Resource
    Electronic Resource
    Glutamine synthetase from mesophyll and bundle sheath maize cells: isoenzyme complements and different sensitivities to phosphinothricin (2000)
    Springer
    Plant cell reports 19 (2000), S. 1127-1134 
    Details
    ISSN: 1432-203X
    Keywords: Key words Bundle sheath cells ; C4 plants ; Glutamine synthetase isoforms ; Mesophyll cells ; Phosphinothricin ; Zea mays L. ; AbbreviationsBSC: Bundle sheath cells ; FPLC: Fast protein liquid chromatography ; GS1: Cytoplasmic glutamine synthetase ; GS2: Chloroplastic glutamine synthetase ; GSt: GS transferase activity ; GSs: GS semibiosynthetic activity ; MC: Mesophyll cells ; 2-ME: 2-Mercaptoethanol ; NADP-ME: NADP-malic enzyme (EC, 1.1.1.41) ; PEPCase: Phosphoenolpyruvate carboxylase (EC, 4.1.1.31) ; PPT: Phosphinothricin ; PVPP: Polyvinilpolypyrrolidone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Anion-exchange FPLC has been used to resolve the isoforms of glutamine synthetase (GS, EC 6.3.1.2) from Zea mays mesophyll (MC) and bundle sheath cells (BSC). Two different isoforms were detected in both types of photosynthetic cells. The predominantly active isoform was GS1 (61%) in MC and GS2 (67%) in BSC. The relative contribution of GS1 and GS2 to the overall GS activity in BSC in maize here reported resembles the proportion described for most C3 plants. Differences among these isoforms in terms of their susceptibility to phosphinothricin (PPT), an analogue of glutamate and known inhibitor of GS, were found. The GS1 isoenzyme from MC was the most sensitive form, being inhibited by 50% at approximately 2.0 μM DL-PPT, whereas the GS2 from BSC presented the highest tolerance to the inhibitor (I50=30 μM). The transferase-to-semibiosynthetic activity ratio for the MC isoforms, which was higher than the ratio for the BSC isoforms, and the differences shown by the isoforms in susceptibility to PPT predict important differences in the biochemical properties and regulation of GS isoenzymes. In this regard, the cytoplasmic isoenzymes, and especially the one in MC, due to its relatively high contribution to mesophyll cell GS activity, could play a vital role in nitrogen metabolism in maize.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000233
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  • 2
    Electronic Resource
    Electronic Resource
    The Transmission Problem of Viscoelastic Waves (2000)
    Springer
    Acta applicandae mathematicae 62 (2000), S. 1-21 
    Details
    ISSN: 1572-9036
    Keywords: transmission problem ; exponential decay ; partially viscoelastic ; materials with memory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract In this paper we consider the transmission problem of viscoelastic waves. That is, we study the wave propagations over materials consisting of elastic and viscoelastic components. We show that for this types of materials the dissipation produced by the viscoelastic part is strong enough to produce exponential decay of the solution, no matter how small is its size. We also show that the linear model is well posed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006449032100
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