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  • phylogeny  (3)
  • Springer  (3)
  • Blackwell Publishing Ltd
  • 2000-2004  (3)
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  • Springer  (3)
  • Blackwell Publishing Ltd
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Nuclear ribosomal ITS sequences and phylogeny in East AsianAconitum subgenusAconitum (Ranunculaceae), with special reference to extensive polymorphism in individual plants (2000)
    Springer
    Plant systematics and evolution 225 (2000), S. 1-13 
    Details
    ISSN: 1615-6110
    Keywords: Ranunculaceae ; Aconitum ; ITS ; polymorphism ; pseudogenes ; phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA (nrDNA) were used to examine the phylogeny of East Asian aconites. Individual aconites were discovered to contain as many as eight different ITS sequences after cloning and PCR-SSCP (single-stranded conformational polymorphisms) analysis. We identified eight putative ITS pseudogenes from four taxa with low predicted secondary structure stability and high substitution rates. Maximum likelihood (ML) and neighbor-joining (NJ) methods were used for phylogenetic reconstruction. The ITS trees agree with the previous chloroplast DNA (cpDNA) tree for the vast majority of the taxa. We found two East Asian clades in the ITS trees: 1) a clade with the Chinese diploid,Aconitum volubile and East Asian tetraploids, and 2) a clade of East Asian diploids and Siberian tetraploids. In the former clade, most tetraploid taxa appear to be polyphyletic; sequences from individual plants did not correspond to recognized taxonomic units. This indicates a recent divergence of the East Asian tetraploids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985455
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  • 2
    Electronic Resource
    Electronic Resource
    Chromosome Painting Technique Contributes to Constructions of Evolutionary Trees of Lemurs (2000)
    Springer
    International journal of primatology 21 (2000), S. 905-913 
    Details
    ISSN: 1573-8604
    Keywords: lemurs ; FISH ; Indridae ; phylogeny ; chromosomal evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We used human-specific probes for chromosomes 3, 11, 14, 15, 17, 19, 21 to paint chromosomes of four species of Indridae (Avahi laniger, Indri indri, Propithecus verreauxi, and P. diadema). All human chromosome probes hybridized specifically to chromosome segments or to whole arms of indrid chromosomes. In general, the results obtained by chromosome painting confirm those obtained by R-banding except for some reciprocal translocations undetected by R-banding. Our findings confirm that in the evolution of the Indridae, Avahi laniger first emerges, then Indri and Propithecus share a common trunk. After populational evolution, this sister clade diverged.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005554911728
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  • 3
    Electronic Resource
    Electronic Resource
    The internal transcribed spacer 2 region of the nuclear ribosomal DNA and the phylogeny of the moss family Hylocomiaceae (2000)
    Springer
    Plant systematics and evolution 224 (2000), S. 127-137 
    Details
    ISSN: 1615-6110
    Keywords: Hylocomiaceae ; internal transcribed region 2 ; molecular evolution ; phylogeny ; relative rate test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nucleotide variation of the internal transcribed spacer 2 region of nuclear ribosomal DNA was used to estimate the phylogeny of the moss family Hylocomiaceae. Both parsimony and neighbour-joining analyses support the monophyly of this family, as well as all seven of the genera within the family.Rhytidium appears as the basal taxon and a North American clade comprisingRhytidiopsis andThelia is indicated. The analyses suggest thatHylocomium s. l., a polyphyletic genus, should be divided into three separate genera. The rate of nucleotide substitutions of the ITS-2 region in the Hylocomiaceae is consistent with the hypothesis of a molecular clock based on a relative rate tests. A nearly constant rate of 6.42 × 10−10 substitutions per site and per year is postulated for the family, a rate close to that of the Winteraceae and slower than that ofLopezia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00986338
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