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  • Polymorphism  (6)
  • Transformation  (4)
  • electrodeposition  (3)
  • Springer  (13)
  • Blackwell Publishing Ltd
  • 2000-2004  (13)
Collection
Keywords
Publisher
  • Springer  (13)
  • Blackwell Publishing Ltd
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Structured diffuse scattering, displacive flexibility and polymorphism in Ba-hexacelsian (2000)
    Springer
    Physics and chemistry of minerals 27 (2000), S. 194-202 
    Details
    ISSN: 1432-2021
    Keywords: Key words Hexacelsian ; Polymorphism ; Diffuse distribution ; Dynamical disorder ; Correlated tetrahedral rotation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Abstract  The results of a temperature-dependent electron diffraction study of the low frequency modes of distortion of Ba-hexacelsian and their relationship to the α-β polymorphic phase transition therein are presented. Cs- and Rb-doped Ba-hexacelsian specimens are also investigated. An extremely strong and characteristic diffuse intensity distribution in the form of polarized sheets of diffuse intensity perpendicular to the 〈1 1 0〉 directions is found for the high temperature polymorph and the doped specimens. The diffuse distribution appears to result from coupled tetrahedral rotation of 〈1 1 0〉 columns of corner-connected (Al,Si)O4 tetrahedra about the [0 0 1] axis (uncorrelated from column to column as a result of the positioning of the rotation axes).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002690050007
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  • 2
    Electronic Resource
    Electronic Resource
    Electrodeposition of cohesive carbon films on aluminum in a LiCl–KCl–K2CO3 melt (2000)
    Springer
    Journal of applied electrochemistry 30 (2000), S. 571-574 
    Details
    ISSN: 1572-8838
    Keywords: aluminum electrode ; carbon film ; electrodeposition ; molten salt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Electrodeposition of carbon on an aluminum electrode was studied in a LiCl–KCl–K2CO3 melt. A cyclic voltammogram for an aluminum electrode indicates that the cathodic current is due to the reduction of CO3 2− ions. Carbon films on aluminum substrates were obtained by potentiostatic electrolysis, and the cohesiveness of the films depended on the potential. SEM observations showed that the morphology of the deposited carbon film depends on the electrolytic conditions. Raman spectroscopy, XPS and XAES measurement showed that the film consisted of carbon in the sp2 state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003927100308
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  • 3
    Electronic Resource
    Electronic Resource
    Electrowinning of tellurium from alkaline leach liquor of cemented Te (2000)
    Springer
    Journal of applied electrochemistry 30 (2000), S. 315-322 
    Details
    ISSN: 1572-8838
    Keywords: alkaline leach liquor ; cemented Te ; electrodeposition ; electrowinning ; tellurium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract The electrochemical behaviour of tellurium in 2.5 M NaOH solution was studied for the recovery of tellurium from alkaline leach liquor of cemented Te using steady state polarization and cyclic voltammetry. The deposition characteristics and the potential range for a stable deposit of tellurium were also investigated. The morphology of deposited Te in alkaline solution showed a very porous nature and needlelike radial growth. The potential range for stable electrodeposition was between −0.8 V and −0.95 V (vs Hg/HgO electrode), but electrowinning could be carried out at more negative potentials due to the disproportionation reaction of Te2 2−. Laboratory-scale electrowinning experiments were performed under different operating voltages, temperatures and initial Te concentrations. The current efficiency was about 85–90% for 50% recovery and about 50–60% for 90% recovery. The purity of electrodeposited Te was higher than 99.95%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003867821601
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  • 4
    Electronic Resource
    Electronic Resource
    Lack of association between atopic asthma and polymorphisms of the histamine H1 receptor, histamine H2 receptor, and histamine N-methyltransferase genes (2000)
    Springer
    Immunogenetics 51 (2000), S. 238-240 
    Details
    ISSN: 1432-1211
    Keywords: Key words Histamine H1 receptor ; Histamine H2 receptor ; Histamine N-methyltransferase ; Polymorphism ; Atopic asthma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050037
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  • 5
    Electronic Resource
    Electronic Resource
    Plant regeneration from hairy roots induced by infection with Agrobacterium rhizogenes in Crotalaria juncea L. (2000)
    Springer
    Plant cell reports 19 (2000), S. 563-568 
    Details
    ISSN: 1432-203X
    Keywords: Key words Agrobacterium rhizogenes ; Crotalaria juncea ; Plant regeneration ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Hairy roots were induced from leaf segments of Crotalaria juncea, which is used as a green manure crop antagonistic to nematodes, by infection with a mikimopine type wild strain of Agrobacterium rhizogenes A13 (MAFF02-10266). These roots exhibited vigorous growth and abundant lateral branching on half-strength Murashige and Skoog (1/2MS) medium without phytohormones. The adventitious shoots were induced from 30% of root segments 3 months after transfer onto medium containing 3 mg/l benzyl adenine. These shoots produced roots 1 month after transfer onto 1.2% agar-solidified 1/2MS medium without phytohormones. Regenerated plants were successfully grown under greenhouse conditions. The transgenic nature of the regenerated plants was confirmed by Southern-blot analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050774
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  • 6
    Electronic Resource
    Electronic Resource
    Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus (2000)
    Springer
    Current genetics 37 (2000), S. 209-212 
    Details
    ISSN: 1432-0983
    Keywords: Key words Basidiomycete ; Transformation ; White-rot fungi ; Carboxin ; Succinate:ubiquinone oxidoreductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel selection marker gene for transformation of the white-rot basidiomycete Pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase. The mutant gene, Cbx R, encodes a modified Ip subunit with an amino-acid substitution (His239 to Leu) and confers resistance to the systemic fungicide, carboxin. The DNA sequence was integrated ectopically in the chromosome of the transformants. This is the first report of a homologous marker gene which is available for the molecular breeding of an edible mushroom.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050521
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  • 7
    Electronic Resource
    Electronic Resource
    The impact of the CYP2D6 and CYP2C19 genotypes on venlafaxine pharmacokinetics in a Japanese population (2000)
    Springer
    European journal of clinical pharmacology 56 (2000), S. 175-180 
    Details
    ISSN: 1432-1041
    Keywords: Key words CYP2D6 ; CYP2C19 ; Polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Objective: The cytochrome P 450 isozymes CYP2D6 and CYP2C19 exhibit genetic polymorphism in human, including a marked interethnic difference. As the functional status of the isozymes CYP2D6 and CYP2C19 have an impact on the pharmacokinetics of some antidepressants, we investigated whether the disposition of venlafaxine was affected by the CYP2D6 and CYP2C19 genotypes. Methods: Twenty-eight adult Japanese men in good health participated in this study. Genomic DNA was isolated from peripheral lymphocytes, and the CYP2D6 genotype was determined using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis and XbaI-RFLP analysis. Subjects were categorized into the following four groups: group 1 CYP2D6*10/*10; group 2 CYP2D6*1/*10 and *2/*10; group 3 CYP2D6*1/*1, *1/*2 and *2/*2; and group 4 the other genotypes. Two defective CYP2C19 alleles (CYP2C19*2 and CYP2C19*3) were identified by means of PCR-RFLP analysis. Venlafaxine was administered orally following an overnight fast. Plasma concentrations of venlafaxine and O-desmethylvenlafaxine were monitored using high-performance liquid chromatography up to 24 h. Results: The peak plasma concentration and values of area under the concentration–time curve up to 24 h for venlafaxine were 298% and 453% higher for group 1 than group 3, and 91% and 120% higher for group 2 than for group 3, respectively. The homozygote for two defective alleles of CYP2C19 showed a higher concentration of venlafaxine within group 1 and group 2. Conclusion: The CYP2D6*10 allele and two CYP2C19 defective alleles, common in an Asian population, are the most likely genetic factors to use in determining interindividual differences in the pharmacokinetics of venlafaxine, although the results with respect to CYP2C19 are preliminary because of the few subjects used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002280050737
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  • 8
    Electronic Resource
    Electronic Resource
    Gene transfer into isolated and cultured tobacco zygotes by a specially designed device for electroporation (2000)
    Springer
    Plant cell reports 19 (2000), S. 1184-1187 
    Details
    ISSN: 1432-203X
    Keywords: Keywords Zygote ; In vitro culture ; Electroporation ; Transformation ; Tobacco ; AbbreviationsNAA: Naphthaleneacetic acid ; ¶BA: 6-Benzyladenine ; GUS: β-Glucuronidase ; ¶GFP: Green fluorescent protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have established a technique for isolating, culturing and transforming tobacco zygotes. Zygotes were isolated by microdissection or enzymatic maceration from fertilized embryo sacs. Viable zygotes cocultured with mesophyll protoplasts underwent first division after 3 days of culture. Zygotes isolated by microdissection underwent a higher frequency of first division (61.2%) than those isolated by enzymatic maceration (30.5%). Globular embryos were formed only from microdissected zygotes, at a frequency of 8.7% after 1–2 weeks in culture. An efficient millicell device for the electroporation of DNA into zygotes was established. The electroporated zygotes divided in vitro at a frequency of 54.6% and developed into proembryos. Introduced GFP gene constructs showed transient expression in about 2.6% of the electroporated tobacco zygotes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000249
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  • 9
    Electronic Resource
    Electronic Resource
    Production of transgenic gentian plants by particle bombardment of suspension-culture cells (2000)
    Springer
    Plant cell reports 19 (2000), S. 454-458 
    Details
    ISSN: 1432-203X
    Keywords: Key words Gentian ; Hygromycin phosphotransferase gene (hpt) ; Particle bombardment ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Cell suspension cultures were established from leaf explants of gentian (Gentiana triflora×G. scabra) for the generation of transgenic plants by particle bombardment. The parameters for the bombardment of suspension culture cells with a particle gun were examined by monitoring the transient expression of a gene for β-glucuronidase driven by the cauliflower mosaic virus (CaMV) 35S promoter. We found that prior culture of suspension culture cells for 5 days on solid medium was optimum for successful particle bombardment. Putative transformed calli were obtained from bombarded cells after a two-step selection procedure. Cells were cultured first with 30 mg l–1 hygromycin in liquid MS medium that contained 10 mg l–1 N-phenyl-N′-1,2,3-thiadiazol-5-yl urea, 1 mg/l 1-naphthaleneacetic acid and 30 g l–1 sucrose and then on solid medium prepared from the same liquid medium plus 2 g l–1 gellan gum. After 12 weeks of selection on solid medium that contained 30 mg l–1 hygromycin, two transgenic gentian plants were regenerated from each selected callus. Analysis by the polymerase chain reaction and Southern blotting revealed the stable integration of transferred DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050755
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  • 10
    Electronic Resource
    Electronic Resource
    The role of electrostatic forces in pollination (2000)
    Springer
    Plant systematics and evolution 222 (2000), S. 133-142 
    Details
    ISSN: 1615-6110
    Keywords: Electric charges ; electrodeposition ; electrostatic pollination ; electrostatic powder coating ; flower morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper reviews research on the role of electrostatic forces in pollination, both in natural and in agricultural systems. Researchers from various fields of biological studies have reported phenomena which they related to electrostatic forces. The theory of electrostatically mediated pollen transfer between insect pollinators and the flowers they visit is described, including recent studies which confirmed that the accumulated charges on airborne honey bees are sufficient for non-contact pollen detachment by electrostatic forces (i.e., electrostatic pollination). The most important morphological features in flower adaptiveness to electrostatic pollination were determined by means of two theoretical models of a flower exposed to an approaching charged cloud of pollen; they are style length and flower opening. Supplementary pollination by using electrostatic techniques is reported, and its possible importance in modern agriculture is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984099
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