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  • Polymorphism
  • Transformation
  • electrodeposition
  • Springer  (42)
  • Blackwell Publishing Ltd
Collection
Keywords
Publisher
  • Springer  (42)
  • Blackwell Publishing Ltd
  • Elsevier  (14)
Years
  • 1
    Electronic Resource
    Electronic Resource
    Sequence-tagged-sites (STSs) of cDNA clones in Cryptomeria japonica and their evaluation as molecular markers in conifers (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 764-772 
    Details
    ISSN: 1432-2242
    Keywords: Key words Linkage map ; RFLP ; STS ; Conifers ; Cryptomeria japonica ; cDNA ; Polymorphism ; Phylogeny ; Homology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have generated 66 sequence-tagged-site (STS) markers from cDNA clones of Cryptomeria japonica, and 60% of them have already been mapped into C. japonica linkage groups. All of the STS markers showed a single fragment following polymerase chain reaction (PCR) amplification. We investigated by polymorphism of these STS markers in a mapped F2 population and 15 plus trees by means of a restriction endonuclease analysis. Polymorphism levels were 10.6% and 22.7% in the F2 population and the 15 plus trees, respectively. PCR amplification levels of the 66 STS markers in 14 conifer species varied depending on their genetic relationship with C. japonica. Taxodium, which is closely related to C. japonica, had the most amplifications (31.82%), followed by Sequoiadendron giganteum, which is of the same family. The average proportion of PCR amplifications in each family gradually declined in the following order: from Taxodiaceae to Cuppresaceae, Sciadopityaceae, Pinaceae, and Taxaceae. These results are in general agreement with a molecular phylogenetic relationship based on chloroplast DNA. The 66 STS markers will be useful as on anchor point for genome mapping and population genetics, and some of them will also be useful when studying other conifers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050476
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  • 2
    Electronic Resource
    Electronic Resource
    Structured diffuse scattering, displacive flexibility and polymorphism in Ba-hexacelsian (2000)
    Springer
    Physics and chemistry of minerals 27 (2000), S. 194-202 
    Details
    ISSN: 1432-2021
    Keywords: Key words Hexacelsian ; Polymorphism ; Diffuse distribution ; Dynamical disorder ; Correlated tetrahedral rotation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Abstract  The results of a temperature-dependent electron diffraction study of the low frequency modes of distortion of Ba-hexacelsian and their relationship to the α-β polymorphic phase transition therein are presented. Cs- and Rb-doped Ba-hexacelsian specimens are also investigated. An extremely strong and characteristic diffuse intensity distribution in the form of polarized sheets of diffuse intensity perpendicular to the 〈1 1 0〉 directions is found for the high temperature polymorph and the doped specimens. The diffuse distribution appears to result from coupled tetrahedral rotation of 〈1 1 0〉 columns of corner-connected (Al,Si)O4 tetrahedra about the [0 0 1] axis (uncorrelated from column to column as a result of the positioning of the rotation axes).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002690050007
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  • 3
    Electronic Resource
    Electronic Resource
    Herbicide-resistant transgenic creeping bentgrass plants obtained by electroporation using an altered buffer (1998)
    Springer
    Plant cell reports 17 (1998), S. 963-967 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAgrostis palustris Huds. ; Protoplast ; Transformation ; Turfgrass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Modification of an electroporation buffer using Ca(NO3)2 and elevated pH (9–10) appeared to have a favorable effect on gene transfer to creeping bentgrass (Agrostis palustris Huds.) Penncross protoplasts, resulting in an increase in the transformation frequency of about twofold. Following electroporation with the plasmid pARK22 containing the bar gene, a total of 278 bialaphos-resistant cell colonies were obtained from four experiments. The bialaphos-resistant regenerants proved to be transgenic by Southern hybridization of the amplified DNA. All the tested transgenic plants showed herbicide (HERBIE) resistance at the field rate of 0.5–1% (vol/vol). Ammonia contents in leaves after spraying with the herbicide increased less in transgenic plants than in untransformed control plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050518
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  • 4
    Electronic Resource
    Electronic Resource
    Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea) (1998)
    Springer
    Plant cell reports 17 (1998), S. 159-164 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cucumber ; Transformation ; Agrobacterium tumefaciens ; Chitinase ; Botrytis cinerea ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rice chitinase cDNA (RCC2) driven by the CaMV 35S promoter was introduced into cucumber (Cucumis sativus L.) through Agrobacterium mediation. More than 200 putative transgenic shoots were regenerated and grown on MS medium supplemented with 100 mg/l kanamycin. Sixty elongated shoots were examined for the presence of the integrated RCC2 gene and subsequently confirmed to have it. Of these, 20 were tested for resistance against gray mold (Botrytis cinerea) by infection with the conidia: 15 strains out of the 20 independent shoots exhibited a higher resistance than the control (non-transgenic plants). Three transgenic cucumber strains (designated CR29, CR32 and CR33) showed the highest resistance against B. cinerea: the spread of disease was inhibited completely in these strains. Chitinase gene expression in highly resistant transgenic strains (CR32 and CR33) was compared to that of a susceptible transgenic strain (CR20) and a control. Different responses for disease resistance were observed among the highly resistant strains. CR33 inhibited appressoria formation and penetration of hyphae. Although CR32 permitted penetration of hyphae, invasion of the infection hyphae was restricted. Furthermore, progenies of CR32 showed a segregation ratio of 3:1 (resistant:susceptible). As the disease resistance against gray mold was confirmed to be inheritable, these highly resistant transgenic cucumber strains would serve as good breeding materials for disease resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050371
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  • 5
    Electronic Resource
    Electronic Resource
    A simple and efficient gene transfer system of trifoliate orange (Poncirus trifoliata Raf.) (1994)
    Springer
    Plant cell reports 13 (1994), S. 541-545 
    Details
    ISSN: 1432-203X
    Keywords: Trifoliate orange ; Epicotyl segment ; Agrobacterium ; Transformation ; rolC promoter ; β-glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple and efficient gene transfer system of trifoliate orange (Poncirus trifoliata Raf.) was developed using epicotyl segments. The segments were infected with Agrobacterium harboring the binary vector pBI121 or pBI101-O12-p1. Both vectors contained the neomycin phosphotransferase II (NPTII) and the β-glucuronidase (GUS) genes. In the plasmid pBI101-O12-p1, the GUS gene was directed to the promoter region of ORF12 (rolC) of the Ri plasmid. On a selection medium containing 100 or 200 μg/ml kanamycin, adventitious shoots were formed from 21.7–44.6% of the segments. Histochemical GUS assay showed that 55.4–87.7% of the shoots expressed the GUS gene. The stable integration of this gene was also confirmed by polymerase chain reaction (PCR) analysis and by Southern blot analysis. When the pBI101-O12-p1 plasmid was used, the GUS activity was found to be located in phloem cells of leaf, stem and root. More than 100 transformed plants were obtained using this method within 2–3 months.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234507
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular changes in protoplast-derived rice plants (1990)
    Springer
    Molecular genetics and genomics 223 (1990), S. 324-328 
    Details
    ISSN: 1617-4623
    Keywords: Rice ; Protoplast ; RFLP ; Methylation ; Polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To determine whether regeneration of rice plants from protoplast culture induces DNA polymorphisms, progeny plants from direct regenerants of such cultures were examined for restriction fragment length polymorphisms (RFLP analysis). Significantly increased levels of DNA polymorphism were found compared with those in non-tissue culture control plants. Analysis with gene sequences representative of different functional domains, revealed that such polymorphisms are apparently widespread and not associated with any particular region. Analysis by comparative digestion with both methylation-sensitive and insensitive restriction enzymes revealed that methylation changes cannot be regarded as a major factor in the induction of these DNA polymorphisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265070
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  • 7
    Electronic Resource
    Electronic Resource
    Electrodeposition of cohesive carbon films on aluminum in a LiCl–KCl–K2CO3 melt (2000)
    Springer
    Journal of applied electrochemistry 30 (2000), S. 571-574 
    Details
    ISSN: 1572-8838
    Keywords: aluminum electrode ; carbon film ; electrodeposition ; molten salt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Electrodeposition of carbon on an aluminum electrode was studied in a LiCl–KCl–K2CO3 melt. A cyclic voltammogram for an aluminum electrode indicates that the cathodic current is due to the reduction of CO3 2− ions. Carbon films on aluminum substrates were obtained by potentiostatic electrolysis, and the cohesiveness of the films depended on the potential. SEM observations showed that the morphology of the deposited carbon film depends on the electrolytic conditions. Raman spectroscopy, XPS and XAES measurement showed that the film consisted of carbon in the sp2 state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003927100308
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  • 8
    Electronic Resource
    Electronic Resource
    pH measurement in the vicinity of a cathode evolving hydrogen gas using an antimony microelectrode (1998)
    Springer
    Journal of applied electrochemistry 28 (1998), S. 617-622 
    Details
    ISSN: 1572-8838
    Keywords: antimony electrode ; microelectrode ; local pH ; electrodeposition ; buffer action ; diffusion layer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract An antimony microelectrode was prepared by quenching a molten Sb–Sb2O3 mixture (2% Sb2O3). The local pH in the vicinity of a cathode evolving hydrogen gas was directly measured using the microelectrode. The local pH during electrolysis of KCl-glycine aqueous solutions was increased by proton consumption; however, the increment decreased with increasing concentrations of glycine, a buffering agent. The diffusion-limiting current density of hydrogen evolution involving proton reduction was controlled by the concentrations of the proton-donating species: protonated-glycine +H3NCH2COOH and H3O+ ions. A plot of the current density against the sum of the concentrations gives a single straight line passing through the origin. The phenomena are discussed in terms of electrodeposition processes of base metals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003254219905
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  • 9
    Electronic Resource
    Electronic Resource
    Electrowinning of tellurium from alkaline leach liquor of cemented Te (2000)
    Springer
    Journal of applied electrochemistry 30 (2000), S. 315-322 
    Details
    ISSN: 1572-8838
    Keywords: alkaline leach liquor ; cemented Te ; electrodeposition ; electrowinning ; tellurium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract The electrochemical behaviour of tellurium in 2.5 M NaOH solution was studied for the recovery of tellurium from alkaline leach liquor of cemented Te using steady state polarization and cyclic voltammetry. The deposition characteristics and the potential range for a stable deposit of tellurium were also investigated. The morphology of deposited Te in alkaline solution showed a very porous nature and needlelike radial growth. The potential range for stable electrodeposition was between −0.8 V and −0.95 V (vs Hg/HgO electrode), but electrowinning could be carried out at more negative potentials due to the disproportionation reaction of Te2 2−. Laboratory-scale electrowinning experiments were performed under different operating voltages, temperatures and initial Te concentrations. The current efficiency was about 85–90% for 50% recovery and about 50–60% for 90% recovery. The purity of electrodeposited Te was higher than 99.95%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003867821601
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  • 10
    Electronic Resource
    Electronic Resource
    An effective transformation method for Hansenula polymorpha (1990)
    Springer
    Current genetics 18 (1990), S. 169-170 
    Details
    ISSN: 1432-0983
    Keywords: Transformation ; Hansenula polymorpha ; Thermotolerant yeasts ; Methylotrophic yeasts ; LEU2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have developed a rapid, simple transformation procedure for intact cells of Hansenula polymorpha. It is a modification of the LiAc method and can yield 104–105 transformants/μg DNA. The use of stationaryphase cells, a high cell density per plate and a hear pulse at 50°C for 10 min are among major modifications of the original method. We have also found that, within the XhoI-SalI chromosomal fragment of Saccharomyces cerevisae containing the LEU2 gene, a sequence exists which supports autonomous replication of plasmid moleculles in H. polymorpha.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312606
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