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  • Articles  (63)
  • Blackwell Publishing Ltd  (62)
  • Public Library of Science
  • 2000-2004  (63)
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  • Articles  (63)
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  • 1
    Electronic Resource
    Electronic Resource
    Modified xylE and xylTE reporter genes for use in Streptomyces: analysis of the effect of xylT (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 196 (2001), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The reporter gene xylE (encoding catechol 2,3-dioxygenase) has been modified for a more rational use in Streptomyces. Two reporter fragments, one containing xylE, and the other containing also the upstream gene xylT (which encodes a soluble ferredoxin), have been constructed to allow precise fusion of regulatory regions to the reporter genes. Identical fusions of these xylE and xylTE reporter fragments to the Streptomyces dagA and tipA promoters, in low and high copy number plasmids, show that the levels of xylE mRNA and catechol 2,3-dioxygenase activities are significantly higher when xylT is present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10569.x
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  • 2
    Electronic Resource
    Electronic Resource
    Mitigation of N2O emissions from grassland by nitrification inhibitor and Actilith F2 applied with fertilizer and cattle slurry (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 18 (2002), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Nitrous oxide (N2O) is involved in both ozone destruction and global warming. In agricultural soils it is produced by nitrification and denitrification mainly after fertilization. Nitrification inhibitors have been proposed as one of the management tools for the reduction of the potential hazards of fertilizer-derived N2O. Addition of nitrification inhibitors to fertilizers maintains soil N in ammonium form, thereby gaseous N losses by nitrification and denitrification are less likely to occur and there is increased N utilization by the sward. We present a study aimed to evaluate the effectiveness of the nitrification inhibitor dicyandiamide (DCD) and of the slurry additive Actilith F2 on N2O emissions following application of calcium ammonium nitrate or cattle slurry to a mixed clover/ryegrass sward in the Basque Country. The results indicate that large differences in N2O emission occur depending on fertilizer type and the presence or absence of a nitrification inhibitor. There is considerable scope for immediate reduction of emissions by applying DCD with calcium ammonium nitrate or cattle slurry. DCD, applied at 25 kg ha–1, reduced the amount of N lost as N2O by 60% and 42% when applied with cattle slurry and calcium ammonium nitrate, respectively. Actilith F2 did not reduce N2O emissions and it produced a long lasting mineralization of previously immobilized added N.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2002.tb00231.x
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  • 3
    Electronic Resource
    Electronic Resource
    Nitrous oxide emissions from grassland in an intensive dairy farm in the Basque Country of Spain (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 17 (2001), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Intensively managed grasslands are potentially a large source of N2O in the North Coast of Spain because of the large N input, the wet soil conditions and mild temperatures. To quantify the effect of fertilizer type and management practices carried out by farmers in this area, field N2O losses were measured over a year using the closed chamber technique. Plots received two types of fertilizer: cattle slurry (536 kg N ha–1) and calcium ammonium nitrate (140 kg N ha–1). N2O losses were less in the slurry treatment than after mineral fertilizer. This was probably due to high, short-lived peaks of N2O encountered immediately following mineral N addition. In contrast, the seasonal distribution of N2O losses from the slurry amended plot was more uniform over the year. The greater N2O losses in the mineral treatment might have been enhanced by the combined effect of mineral fertilizer and past organic residues present from previous organic amendments. Weak relationships were found between N2O emission rates and soil nitrate, soil ammonium, soil water content and temperature. Better relationships were obtained in the mineral treatment than in the slurry plots, because of the wider range in soil mineral N. Water filled pore space (WFPS) was a key factor controlling N2O emissions. In the 〉 90% WFPS range no relationships were found. The best regressions were found for the mineral treatment in the 40–65% WFPS range, 49% of the variance being explained by soil nitrate and ammonium content. In the 65–90% WFPS range, 43% of the variance was explained by nitrate only, but the inclusion of soil ammonium did not improve the model as it did in the 40–65% WFPS range. This fact indicates that nitrification is likely to be an important process involved in N2O emissions at the 40–65% WFPS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2001.tb00017.x
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  • 4
    Electronic Resource
    Electronic Resource
    MICROBIAL ENUMERATION IN READY-TO-EAT FOODS AND THEIR RELATIONSHIP TO GOOD MANUFACTURING PRACTICE (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 22 (2002), S. 0 
    Details
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: As a contribution to Good Manufacturing Practice (GMP) programs, we have analyzed products produced and offered by ready-to-eat food stores. Two hundred and seventy four samples of a wide variety of foods from 19 different shops were analyzed. Aerobic counts, total coliforms and yeast and molds were enumerated in each sample. Food stores were evaluated using a GMP check-list. They were grouped in three classes: Class III, nonsatisfactory GMP; Class II, partially satisfactory GMP; and Class I, satisfactory GMP. From the results of microorganism counts in the Class I food shops the following maximum counts are proposed for cooked ready-to-eat foods: aerobic colony count: 105 cfu g−1. It is coincident with satisfactory microbiological quality of many category 3 foods drawn up by Public Health Laboratory Service (PHLS).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4565.2002.tb00328.x
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  • 5
    Electronic Resource
    Electronic Resource
    DESCRIPTION OF THE SENSORY CHARACTERISTICS OF SPANISH UNIFLORAL HONEYS BY FREE CHOICE PROFILING (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 18 (2003), S. 0 
    Details
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Different Spanish unifloral honeys (eucalyptus, sunflower, rosemary, thyme, lavender, citrus, anise, quercus, and lemon blossom) and one multifloral honey were studied by Free-Choice Profiling (FCP) analysis. Generalized Procrustes Analysis (GPA) applied to the FCP data allowed discrimination between samples and provided information on the attributes responsible for the differences observed. The honeys had significantly different sensory characteristics. Textural attributes were the predominant factor in discriminating between samples, and appearance (color included) was also correlated with GPA dimensions to a lesser extent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-459X.2003.tb00378.x
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  • 6
    Electronic Resource
    Electronic Resource
    Evaluation of polygalacturonase activity in Saccharomyces cerevisiae wine strains (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 237 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A total of 61 S. cerevisiae strains, 60 of them isolated from wine ecosystems, were evaluated for the presence of the gene encoding endopolygalacturonase (PGU1) and for polygalacturonase (PG) activity. Nine strains lack the gene PGU1 and did not exhibit PG activity on plate assays. Of the 52 strains showing an amplified band corresponding to the size of PGU1 gene, only 36 degraded polygalacturonic acid (PGA) and 17 did not degrade it at any of the pH values used. The coding region of the PGU1 gene (ORF YJR153w) was not present in some PG activity negative strains. The S. cerevisiae UCLMS-39 strain was selected for its specific activity at different pHs, temperatures and oenological parameters. The temperature and pH optima were 50 °C and 3.5–5.5 respectively and it was only affected by ethanol. The PGU1 gene was cloned and sequenced. The production of a biologically functional endoPG in S. cerevisiae UCLMS-39 brings us a step closer to improving the qualities of outstanding enological yeasts naturally lacking PG activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2004.tb09705.x
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  • 7
    Electronic Resource
    Electronic Resource
    A novel glutamate-dependent acid resistance among strains belonging to the Proteeae tribe of Enterobacteriaceae (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 237 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Morganella, Providencia and Proteus strains were capable of surviving pH 2.0 for 1 h if glutamate was present. These strains did not have glutamic acid decarboxylase activity and the gadAB genes were not detected in any of these bacteria. When exposed to pH 2.0 acid shocks, the survival rate of these bacteria was significantly increased with glutamate concentrations as low as 0.3 mM in the acid media. Escherichia coli cells incubated at pH 3.4 consumed four times more glutamate and produced at least 7-fold more γ-amino butyric acid than Morganella, Providencia and Proteus strains. These results indicate that strains belonging to the Proteeae tribe might have novel glutamate dependent acid-resistance mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2004.tb09711.x
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  • 8
    Electronic Resource
    Electronic Resource
    A Statistically Based Strategy for Obtaining Equilibrium Data in Xanthophylls Lixiviation (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 66 (2001), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : The design of many separation processes is often hindered by the lack of equilibrium data. In recent years, statistically based experimental design has been successfully used to quantify relevant factors in many biological and chemical processes. We illustrate the capabilities of one of these approaches in obtaining equilibrium data for the design of lixiviation processes. The study case involves the lixiviation of marigold flower flour for xanthophylls extraction using hexane in a multistage countercurrent system. The method leads to conditions that allow the recovery of 95.3% of the pigment in seven stages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2001.tb16095.x
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  • 9
    Electronic Resource
    Electronic Resource
    Instrumental and Expert Assessment of Mahon Cheese Texture (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 65 (2000), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : To improve Mahon cheese texture assessment, the relationship between instrumental and sensory measurements was sought. For that purpose 30 pieces of Mahon cheese from different batches and 2 different manufacturers were examined. Textural characteristics at different curing times were evaluated by uniaxial compression, puncture, and sensory analysis. Significant linear correlations were found between instrumental and sensory measurements. A logarithmic model (Weber-Fechner) fitted data better than a linear one. Only 1 factor was extracted when considering all the instrumental and sensory variables, thus indicating that both sets of measurements are related to the same phenomenon. The best predictors for Mahon cheese sensory attributes were found to be cheese moisture, deformability modulus, and slope in puncture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2000.tb10260.x
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  • 10
    Electronic Resource
    Electronic Resource
    NITROGEN AND ORGANIC MATTER LOSSES IN NO-TILL CORN CROPPING SYSTEMS (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 40 (2004), S. 0 
    Details
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : Intensive cropping systems based on mechanical movement of soil have induced land degradation in most agricultural areas due to soil erosion and soil fertility losses. Thus, farmers have been increasing fertilization rates to maintain an economically competitive crop yield. This practice has resulted in water quality degradation and lake eutrophication in many agricultural watersheds. Research was conducted in the Patzcuaro watershed in central Mexico to develop appropriate technology that prevents nonpoint source pollution from fertilizers. Organic matter (OM) and nitrogen (N) losses in runoff and nitrate (NO3-N) percolation in Andisols with corn under conventional till (CT) and no-till (NT) treatments using variable percentages of crop residue as soil cover were investigated for steep-slope agriculture. USLE type runoff plots were used to collect water runoff, while suction tubes with porous caps at 30, 60, and 90 cm depth were used to sample soil water solutes for NO3-N analyses. Results indicated a significant reduction of N and OM losses in runoff as residue cover increased in the NT treatments. Inorganic N in runoff was 25 kg/ha for NT without residue cover (NT-0) and 6 kg/ha for the NT with 100 percent residue cover (NT-100). Organic matter losses in runoff were 157 and 24 kg/ha for the NT-0 and NT-100 treatments, respectively. Nitrate-N percolation was evident in CT and NT with 100 percent residue cover (NT-100). However, NT-100 had higher NO3-N concentration at the root zone, suggesting the possibility of reducing fertilization rates with the use of NT treatments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1752-1688.2004.tb01038.x
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