Publication Date:
1999-12-11
Description:
Human corneal equivalents comprising the three main layers of the cornea (epithelium, stroma, and endothelium) were constructed. Each cellular layer was fabricated from immortalized human corneal cells that were screened for use on the basis of morphological, biochemical, and electrophysiological similarity to their natural counterparts. The resulting corneal equivalents mimicked human corneas in key physical and physiological functions, including morphology, biochemical marker expression, transparency, ion and fluid transport, and gene expression. Morphological and functional equivalents to human corneas that can be produced in vitro have immediate applications in toxicity and drug efficacy testing, and form the basis for future development of implantable tissues.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Griffith, M -- Osborne, R -- Munger, R -- Xiong, X -- Doillon, C J -- Laycock, N L -- Hakim, M -- Song, Y -- Watsky, M A -- New York, N.Y. -- Science. 1999 Dec 10;286(5447):2169-72.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉University of Ottawa Eye Institute and Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa Hospital-General Campus, Ottawa, Ontario K1H 8L6, Canada. mgriffith@ogh.on.ca〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10591651" target="_blank"〉PubMed〈/a〉
Keywords:
Animal Testing Alternatives
;
*Biomedical Engineering
;
Cell Line
;
Cells, Cultured
;
Chondroitin Sulfates
;
Collagen
;
*Cornea/cytology/growth & development/physiology
;
Corneal Opacity/chemically induced
;
Corneal Stroma/cytology/growth & development/physiology
;
Corneal Transplantation
;
Cross-Linking Reagents
;
*Culture Techniques
;
Electrophysiology
;
Endothelium, Corneal/cytology/growth & development
;
Epithelium, Corneal/cytology/growth & development
;
Gene Expression
;
Glutaral
;
Humans
;
Ion Channels
;
Ouabain/pharmacology
;
Patch-Clamp Techniques
;
Sodium Dodecyl Sulfate/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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