ALBERT

Notes: We analyse a puzzle in the UK corporation tax: by both historic and international standards, corporation tax revenues have been high while the statutory rate has been reduced. We consider explanations based on changes in the tax law and in economic factors. Changes in the tax law, such as base-broadening measures through reductions in capital allowances, can explain only part of the puzzle. Among the economic explanations, an increase in the size of the corporate sector, mainly caused by expansion of the service sector and improvements in profitability of the financial sector, seems the most likely. To the extent that higher profits, particularly financial sector profits, may have led to high revenues, there are doubts as to whether revenues will continue to be so strong.

Notes: Cross contamination by microbial pathogens in the kitchen environment may play an important role in many cases of food borne illnesses. Hand washing has been shown to be one of the most important factors in controlling the spread of microorganisms and in preventing the spread of disease. However, educational campaigns such as distribution of information leaflets, workshops, performance feedback and lectures have been, at best, associated with a transient improvement in compliance rates. In addition, the majority of research investigating UK consumers’ food safety behaviour has examined self-reported as opposed to actual hand washing behaviour.This research utilises psychological theory in an attempt to understand how one might design a more effective hand washing campaign. Social cognition models were utilised to explore the relationship between consumers’ knowledge, attitudes, self-reported and actual hand washing behaviour. The research was conducted in two stages. Firstly, salient beliefs of 100 consumers towards food safety were obtained using open-ended questions. Secondly, the food handling practices of 40 consumers were observed and their food safety attitudes and knowledge determined using structured questionnaires.All the participants were knowledgeable about hand washing techniques, intended to wash their hands and generally had positive attitudes towards the importance of washing their hands. However, none of the participants adequately washed their hands on all appropriate occasions. The attitude statement results suggest measures of perceived behavioural control, perceived barriers and perceived risk may provide developers of food safety intervention materials with more useful information compared with measures of consumers’ knowledge or intention. Issues of habit and optimistic bias also need to be given consideration when designing intervention materials to change hand washing behaviour of consumers.

Notes: Worldwide incidence of food-borne disease has increased in recent years and data suggest that inadequate food-handling behaviour in the domestic environment may be an important factor. As a consequence of this, research into aspects of consumer food safety has been undertaken, especially in the UK and USA. The overall aims of conducting such research have been to investigate aspects of consumer food safety behaviour and to utilize information in the development of effective food safety education initiatives. In the past 25 years, 87 consumer food safety studies have been undertaken using different research methodologies; 75% utilized survey techniques (questionnaires and interviews), 17% were based on direct observation, and 8% utilized focus groups. The advantages and disadvantages of the different research methods used are discussed. Similarly, different forms of reliability and validity have been considered in the context of each research method used. A comparison of results from consumer food safety studies has shown that use of different research designs and approaches has resulted in differences in the findings about consumer food safety behaviour. Survey responses have provided a more optimistic portrayal of consumer food safety behaviour than data obtained from focus groups and direct observation. Although consumers have demonstrated knowledge, positive attitudes and intentions to implement safe practices, substantially larger proportions of consumers have been observed to implement frequent malpractices. This suggests that observational data provide the most reliable information denoting consumers’ actual food safety behaviour and should be used preferentially with risk-based data for the design of communication strategies.

Notes: The domestic kitchen is an important point of origin for foodborne disease and food safety communication strategies are required to improve food-handling behaviors in the home. A key to the design of effective strategies is an understanding of factors that influence food safety behaviors. Attitudes may influence food safety behaviors and therefore, attitude determination towards food safety in the domestic kitchen is important for development of targeted food safety communication strategies. A questionnaire was administered to consumers (n = 100) representing a cross-section of the population of Cardiff, South Wales. Results showed that attitudes towards implementation of key food safety behaviors including cross contamination, cooking and storage were positive, however, attitudes towards other practices, for example cooling, were negative. Significant correlations (P 〈 0.05) between specific attitudinal responses and respondent demographics were identified, indicating that targeted strategies may be required for different groups of consumers. Results of this study will be discussed within the context of previous consumer food safety research, optimistic-bias and development of communication strategies designed to raise awareness of food safety issues and bring about behavioral improvement.

Notes: SoxS is the direct transcriptional activator of at least 15 genes of the Escherichia coli superoxide regulon. SoxS is small (107 amino acids), binds DNA as a monomer and recognizes a highly degenerate DNA binding site, termed ‘soxbox’. Like other members of the AraC/XylS family, SoxS has two putative helix–turn–helix (HTH) DNA-binding motifs, and it has been proposed that each HTH motif recognizes a highly conserved recognition element of the soxbox. To determine which nucleotides are important for SoxS binding, we conducted a systematic mutagenesis of the DNA binding sites for SoxS in the zwf and fpr promoters and determined the effect of the soxbox mutations on SoxS DNA binding and transcription activation in vivo by measuring β-galactosidase activity in strains with fusions to lacZ. We found that the sequences GCAC and CAAA, termed recognition elements 1 and 2 (RE 1 and RE 2), respectively, are critical for SoxS binding, as mutations within these elements severely hinder or eliminate SoxS-dependent transcription activation; substitutions within RE 2 (CAAA), however, are tolerated better than changes within RE 1 (GCAC). Although substitutions at the seven positions separating the two REs had only a modest effect on SoxS binding, AT basepairs were favoured within this ‘spacer’ region, presumably because, by facilitating DNA bending, they help bring the two recognition elements into proper juxtaposition. We also found that the ‘invariant A’ present at position 1 of 14/15 functional soxboxes identified thus far is important for SoxS binding, as a change to any other nucleotide at this position reduced SoxS-dependent transcription by ≈ 50%. In addition, positions surrounding the REs seem to show a context effect, in that certain substitutions there have little or no effect when the RE has the optimal binding sequence, but produce a pronounced effect when the RE has a suboptimal sequence. We propose that these nucleotides play an important role in effecting differential expression from the various promoters. Lastly, we used gel retardation assays to show that alterations in transcription activation in vivo are caused by effects on DNA binding. Based on this exhaustive mutagenesis, we propose the following optimal sequence for SoxS binding: AnVGCACWWWnKRHCAAAHn (n = A, C, G, T; V = A, C, G; W = A, T; K = G, T; R = A, G; H = A, C, T).

Notes: Haemophilus influenzae (Hi), a commensal of the human respiratory mucosa, is an important cause of localized and systemic infections. We show that distinct strains belonging to typable (THi) and non-typable (NTHi) H. influenzae target human carcinoembryonic antigens (the membrane associated CEA family of cell adhesion molecules, are now termed CEACAMs). All strains of H. influenzae biogroup aegyptius (Hi-aeg) and more than 70% of THi and NTHi strains tested specifically recognize CEACAMI-Fc soluble constructs. Furthermore, transfection of Chinese hamster ovary cells with human CEACAM1 cDNA alone was sufficient for promoting Hi interactions with the transfected cells. The majority of the Hi-aeg strains tested interacted with soluble constructs containing only the N-terminal domain. In contrast, several THi and NTHi strains reacted with soluble constructs only when additional extracellular A and B domains of the receptor were present. The use of monoclonal antibodies confirmed that THi and NTHi strains also interact primarily at the N-domain. We used site-directed mutants of CEACAM1 that contained substitutions at surface exposed amino acids and a molecular model of the N-domain to identify the residues involved in interactions with Hi ligands. The studies show that a common region exposed at the CFG face of the molecule is targeted by diverse Hi strains. However, mutation at distinct sites within this area affected the interactions of distinct strains signifying the potential for tissue tropism via this receptor. Analyses of the molecular basis of interaction with human cell lines and purified CEA show that Hi strains, especially those belonging to Hi-aeg, interact with multiple CEACAMs. Because Neisseria meningitidis (Nm) strains are also known to bind at the CFG face of the receptor, we used Nm and Hi strains in co-infection experiments and demonstrate competition between these mucosal pathogens in colonization of target cells via CEACAMs.

Notes: In Escherichia coli, the SoxRS regulon confers resistance to redox-cycling compounds, and the Mar regulon provides a defence against multiple antibiotics. The response regulators, SoxS and MarA, are synthesized de novo in response to their inducing signals and directly activate transcription of a common set of target genes. Although the mechanisms of transcription activation by SoxS and MarA have been well studied, little is known about how the systems are shut-off once the inducing stress has subsided, except that de novo synthesis of the regulators is known to cease almost immediately. Here, we induced the SoxRS regulon and determined that, upon removal of the inducer, expression of the regulon's genes quickly returns to the preinduced level. This rapid shut-off indicates that the system is reset by an active process. We found that SoxS is unstable and infer that SoxS degradation is responsible for the rapid return of the system to the ground state upon removal of the inducing signal. We also found that MarA is unstable and that the instability of both proteins is intrinsic and unregulated. We used null mutations of protease genes to identify the proteases involved in the degradation of SoxS and MarA. Among single protease mutations, only lon mutations increased the half-life of SoxS and MarA. In addition, SoxS appeared to be nearly completely stable in a lon ftsH double mutant. Using hexahistidine tags placed at the respective ends of the activators, we found that access to the amino-terminus is essential for the proteolytic degradation.

Notes: Petroleum and mineral exploration and extraction, hydroelectric development, atmospheric transport of contaminants, timber harvesting and tourism are increasing worldwide, especially in the Arctic. This development may adversely affect populations of reindeer/caribou (Rangifer tarandus) which are the basis of subsistence economies for northern indigenous peoples. Our purpose is to present a survey of the literature that has investigated the response of reindeer/caribou to human activities. Individuals and groups of reindeer/caribou: 1) move away from point sources of disturbance; 2) increase activity and energy expenditure near disturbance; 3) delay crossing or fail to cross linear structures; 4) shift away from areas of extensive and intensive development; and 5) are killed by collisions with vehicles and by hunting along roads. Cows and calves during the calving season are the most easily disturbed group. Bulls in general and all reindeer/caribou during insect harassment are least likely to avoid development areas. Estimation of the proportion of a population that is exposed to disturbance and the implications of this exposure to annual energy budgets, survival and productivity of reindeer/caribou has received little attention. Future advances in understanding the implications of human disturbance to reindeer/caribou will require cumulative effects assessment at annual, population and regional scales. Although some level of cumulative effect is likely, clear separation of cumulative effects of development from natural variation in caribou habitat use and demography will be difficult.

Notes: The importance of migratory caribou (Rangifer tarandus) to northerners, the increasing pressure to extract non-living resources, and predicted global climate change have led researchers, managers and resource users alike to focus on how to improve our knowledge of this unique northern ungulate. Unprecedented threats to caribou sustainability, along with the increasingly acknowledged value of indigenous hunters’contribution to caribou research, pose the additional challenge to innovate research methods that accommodate differing cultural perspectives and facilitate communication among groups. This paper surveys the state of scientific knowledge of the eleven major northern mainland herds of North America. We recommend an approach to improve our working knowledge of barren-ground caribou in order to assess better future impacts. The transfer of knowledge gained from years of research and indigenous experience on many aspects of caribou ecology should be evaluated and, where applicable, transferred to herds with more modest databases. The establishment of a North American Caribou Monitoring and Assessment System, based on a synthesis of local knowledge and research-based science, is recommended as a tool for improved communication and collective learning.