ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

Electronic Resource

RECOVERY OF ESCHERICHIA COLI O157:H7 FROM FIBER OPTIC WAVEGUIDES USED FOR RAPID BIOSENSOR DETECTION (2002)

KRAMER, MARIANNE F. ; TIMS, THOMAS B. ; DeMARCO, DANIEL R. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Journal of rapid methods and automation in microbiology 10 (2002), S. 0

add to mindlist on the mindlist

Details

ISSN: 1745-4581

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Consumption of ground beef has been implicated in the majority of outbreaks of disease from Escherichia coli O157:H7. There is a great need for rapid, sensitive, and specific microbial detection and isolation methods for the detection and recovery of pathogens such as E. coli O157:H7. Evanescent wave, fiber optic biosensors are an innovative, cutting-edge technology with the potential to meet such a need. Food pathogens such as E. coli O157:H7 can be detected in minutes using the biosensor assay rather than days using conventional methods. The biosensor assay is sensitive and presently has the ability to directly detect 102 CFU of E. coli O157:H7 in E. coli O157:H7-seeded PBS or ground beef. In this study, it was determined that once the pathogen had been positively identified, it could then be recovered by culturing the cells captured on the waveguide used in the biosensor assay. E. coli O157:H7-seeded PBS or ground beef samples were assayed using an evanescent wave, fiber optic biosensor. After a positive identification on the biosensor, the waveguide was added to modified LB medium supplemented with 10 μg/mL acriflavin (mLB) and incubated at 42C for a minimum of 4 h followed by selection on Rainbow O157 agar incubated 16 to 18 h at 37C. E. coli O157:H7 was confirmed using agglutination with E. coli O157:H7 antiserum. It was possible to recover E. coli O157:H7 colonies in E. coli O157:H7-seeded PBS or ground beef when 1 mL samples containing at least 2.0 × 103 CFU of E. coli O157:H7 had been injected into the biosensor. It took only 24 h to detect the target pathogen, recover the pathogen from the sample, and grow isolated colonies. This total time included sample preparation, detection, enrichment culture, and growth of cells to produce isolated colonies on a selective and differential medium. The identification of isolated colonies was then quickly confirmed using slide agglutination with the appropriate antiserum. This method significantly improved the speed of the confirmation of the presence of the pathogen and of the isolation of the pathogen, from 4 days using conventional methods to one day using the biosensor assay.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1745-4581.2002.tb00015.x

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

2

Electronic Resource

Seed dispersal and movement patterns in two species of Ceratogymna hornbills in a West African tropical lowland forest (2000)

Holbrook, Kimberly M. ; Smith, Thomas B.

Springer

Oecologia 125 (2000), S. 249-257

add to mindlist on the mindlist

Details

ISSN: 1432-1939

Keywords: Seed dispersal Seed shadow Hornbills Telemetry Tropical frugivory

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract. We studied two species of Ceratogymna hornbills, the black-casqued hornbill, C. atrata, and the white-thighed hornbill, C. cylindricus, in the tropical forests of Cameroon, to understand their movement patterns and evaluate their effectiveness as seed dispersers. To estimate hornbill contribution to a particular tree species' seed shadow we combined data from movements, determined by radio-tracking, with data from seed passage trials. For 13 individuals tracked over 12 months, home range varied between 925 and 4,472 ha, a much larger area than reported for other African avian frugivores. Seed passage times ranged from 51 to 765 min, with C. atrata showing longer passage times than C. cylindricus, and larger seeds having longer gut retention times than smaller seeds. Combining these data, we estimated that seed shadows were extensive for the eight tree species examined, with approximately 80% of seeds moved more than 500 m from the parent plant. Maximum estimated dispersal distances for larger seeds were 6,919 and 3,558 m for C. atrata and C. cylindricus, respectively. The extent of hornbill seed shadows suggests that their influence in determining forest structure will likely increase as other larger mammalian dispersers are exterminated.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004420000445

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

3

Electronic Resource

New ways of determining structural groups in brown coals and their bioconversion products by FT IR spectroscopy (2000)

Weber, A. ; Tesch, S. ; Thomas, B. ; [et al.]

Springer

Applied microbiology and biotechnology 54 (2000), S. 681-685

add to mindlist on the mindlist

Details

ISSN: 1432-0614

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract New methods of determining the structural groups —COOH and —CH2— have been developed. The investigation of carboxyl groups is possible both after derivatization with p-fluorophenacylbromide and by quantitative interpretation of the Fourier transform infrared (FT IR) spectra. There exists a linear relationship between the results of these two methods that is generally valid for the analysis of all brown coal components. The maximum extinction coefficient of the symmetric stretching vibration band of the CH2 groups has been determined using model substances. This allows quantification of this structural group directly from the FT IR spectrum. The results agree with the contents of methylene groups as determined by 13C-cross polarization–magic angle spinning–nuclear magnetic resonance (13C CPMAS NMR) spectroscopy. Using these methods, the COOH and CH2 groups contained in brown coals of the North Rhine region and in their bioconversion products have been quantified.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002530000419

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

4

Electronic Resource

Die Wirkungen der totalen Leberexstirpation (1924)

Mann, Frank C. ; Magath, Thomas B.

Springer

Reviews of physiology, biochemistry and pharmacology 23 (1924), S. 212-273

add to mindlist on the mindlist

Details

ISSN: 1617-5786

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: Zusammenfassung Kurz zusammengefasst sind die wichtigsten, hier niedergelegten Tatsachen folgende: Es wurde eine Methode für die totale Entfernung der Leber bei Hunden ausgebildet, welche frei von den meisten Fehlerquellen ist, die früheren Exstirpationsmethoden des Organs bei Säugetieren anhaften. Es wurde gefunden, dass eine geeignete Einführung von Glukose bei derart operierten Hunden das Leben viele Stunden nach Verlust des gesamten Lebergewebes erhält. Von den vielen an hepatektomierten Tieren gemachten Beobachtungen möchten wir hervorheben, 1. dass die Leber zur Erhaltung des Blutzuckerspiegels absolut wesentlich ist; 2. dass die Leber der wichtigste Faktor, wenn nicht der einzige, bei dem Prozess der Desamidierung und der Harnstoffbildung ist, und 3. dass Bilirubin auch ohne Mitwirkung der Leber gebildet werden kann.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01924578

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

5

Electronic Resource

Distribution and Variability of Surface Soil Properties at a Regional Scale (2000)

Brejda, John J. ; Moorman, Thomas B. ; Smith, Jeffrey L. ; [et al.]

Springer

Soil Science Society of America journal 64 (2000), S. 974-982

add to mindlist on the mindlist

Details

ISSN: 1435-0661

Keywords: MBC, microbial biomass carbon MEP, Mehlich extractable phosphorus MLRA, Major Land Resource Areas MWD, mean-weight diameter NRCS, Natural Resources Conservation Service NRI, National Resource Inventory PMN, potentially mineralizable nitrogen PSU, primary sampling units TOC, total organic carbon WSA, water stable aggregates

Source: Springer Online Journal Archives 1860-2000

Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: e transformation resulted in normal distributions for most soil properties and reduced variability two- to threefold. However, a few soil properties remained non-normally distributed. Soil pH may be monitored at the regional scale with a high degree of precision. Small changes in soil C content (3–8% of the regional mean) may be detected using loge transformed total organic C as the indicator. Sampling soil properties as part of the NRI should improve NRCS' ability to monitor soil condition on a regional scale.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

6

Electronic Resource

Phosphorylation of Tau Alters Its Association with the Plasma Membrane (2000)

Ekinci, Fatma J. ; Shea, Thomas B.

Springer

Cellular and molecular neurobiology 20 (2000), S. 497-508

add to mindlist on the mindlist

Details

ISSN: 1573-6830

Keywords: tau ; phosphorylation ; signal transduction ; protein kinase C ; Alzheimer's disease

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract 1. The potential functions of the microtubule-associated protein tau have been expanded by the recent demonstration of its interaction with the plasma membrane. Since the association of tau with microtubules is regulated by phosphorylation, herein we examine whether or not the association of tau with the plasma membrane is also regulated by phosphorylation. 2. A range of tau isoforms migrating from 46 to 64 kDa was associated with crude particulate fractions derived from SH-SY-5Y human neuroblastoma cells, and were retained during the initial stages of plasma membrane purification. During the extensive washing utilized in purification of the plasma membrane, portions of each of these isoforms were depleted from the resultant purified membrane. Immunoblot analysis with phospho-dependent and -independent antibodies revealed selective depletion of phospho isoforms during membrane washing. This effect was more pronounced for the slowest-migrating (64-kDa) tau isoform. 3. This putative influence of phosphorylation on the association of tau with the plasma membrane was further probed by transfection of SH-SY-5Y human neuroblastoma cells with a tau construct that could associate with the plasma membrane but not with microtubules. Treatment with phorbol ester or calcium ionophore, both of which increased phospho-tau levels within the cytosol and plasma membrane, was accompanied by the dissociation of this tau construct from the membrane. 4. These data indicate that phosphorylation regulates the association with the plasma membrane. Dissociation from the membrane by phosphorylation may place tau at risk for hyperphosphorylation and ultimate PHF formation in a manner previously considered for tau dissociated from microtubules.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1007075115574

Permalink