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  • Key words: Cysteine protease inhibitor – Oryzacystatin – Protein targeting – Signal peptide  (1)
  • biodiversity  (1)
  • Springer  (2)
  • American Association of Petroleum Geologists (AAPG)
  • American Meteorological Society
  • American Physical Society
  • Periodicals Archive Online (PAO)
  • 2000-2004  (2)
  • 1940-1944
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  • Springer  (2)
  • American Association of Petroleum Geologists (AAPG)
  • American Meteorological Society
  • American Physical Society
  • Periodicals Archive Online (PAO)
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  • 2000-2004  (2)
  • 1940-1944
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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Planta 210 (2000), S. 844-847 
    ISSN: 1432-2048
    Schlagwort(e): Key words: Cysteine protease inhibitor – Oryzacystatin – Protein targeting – Signal peptide
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract.  A previously unidentified extension of an open reading frame from the genomic DNA of Japonica rice (Oryza sativa L.) encoding oryzacystatin-I (OC-I; access. M29259, protein ID AAA33912.1) has been identified as a 5′ gene segment coding for the OC-I signal peptide. The signal peptide appears to direct a pre-protein (SPOC-I; Accession No. AF164378) to the endoplasmic reticulum, where it is processed into the mature form of OC-I. The start codon of SPOC-I begins 114 bp upstream from that previously published for OC-I. A putative proteolytic site, which may yield a mature OC-I approximately 12 residues larger than previously described, has been identified within SPOC-I between Ala-26 and Glu-27. The signal peptide sequence was amplified by polymerase chain reaction using genomic DNA from O. sativa seedlings and ligated to the 5′ end of the truncated OC-I gene at the endogenous SalI site. Partially purified protein extracts from Escherichia coli expressing SPOC-I reacted with polyclonal antibodies raised against OC-I and revealed a protein of the expected molecular weight (15,355 Da). In-vitro translation of SPOC-I in the presence of microsomal membranes yielded a processed product approximately 2.7 kDa smaller than the pre-protein. Nicotiana tabacum L. cv. Xanthi plants independently transformed with the SPOC-I gene processed SPOC-I and accumulated the mature form of OC-I (approximately 12.6 kDa), which co-migrated with natural, mature OC-I extracted from rice seed when separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    ISSN: 1572-9710
    Schlagwort(e): beetles ; biodiversity ; cloud forest ; conservation ; Curculionidae ; Honduras ; Staphylinidae ; tropical forest
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Thirteen tropical montane cloud forests (TMCFs) in Honduras and adjacent El Salvador were evaluated for species diversity and endemism in leaf litter inhabiting Curculionidae (weevils) and Staphylinidae in June and August of 1994. Totals of 26,891 specimens representing 293 species of Curculionidae, and 7349 specimens representing 224 species of Staphylinidae, were collected. Regional endemism was high with 173 species (58.7% of total) of Curculionidae and 126 species (56.3% of total) of Staphylinidae, restricted to single sites. Measures of diversity (number of observed species [Sobs]; number of endemic species) and estimates of biodiversity (Chao 2, first and second order Jackknife and Bootstrap richness estimators) are given for each site for Curculionidae and Staphylinidae. Priority-areas analyses based on Sobs (‘Greedy’), Sobs, Chao 2, number of endemic species, site complementarity and higher taxonomic diversity (Curculionidae only) are presented. Weak or lack of correlation was noted between site area and site diversity for either Curculionidae or Staphylinidae. The optimum sequence for site conservation was determined based on Sobs (‘Greedy’). Four sites are identified as the highest conservation priorities; Parque Nacional Montecristo, P.N. La Muralla, P.N. Santa Barbara and P.N. Comayagua. Identification as high priority sites supports their designation as Parques Nacionales rather than the less diverse Reservas Biologias or Reservas Vidas Silvestres. While closely approximating performance of Sobs (‘Greedy’), no one of Sobs, number of endemics, Chao 2 or site complementarity give results equivalent to the optimum sequence based on Sobs (‘Greedy’) and the latter is concluded to be the best method for establishing conservation priorities in TMCF. Results of the analyses based on one of Curculionidae or Staphylinidae differ in the ordering of site priorities based on each of Sobs, Chao 2 or number of endemics at each site, with at best, weak positive correlations between results based on each taxon. Data from other taxa are necessary to determine if one of Curculionidae or Staphylinidae emerges as the better surrogate for general patterns of biodiversity in TMCF. Conservation strategies in Central America should emphasize the importance of TMCF particularly in view of high regional endemism. In formulating these strategies, consideration needs to be given to the preservation of many small regional preserves rather than fewer larger preserves. Data from inventories of other taxa should be included where available and all biological data should be integrated with social and cultural issues of regional importance.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
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