Publication Date:
2002-12-10
Description:
Spermatogenesis is a highly specialized process of cellular differentiation to produce spermatozoa. This differentiation process accompanies morphological changes that are controlled by a number of genes expressed in a stage-specific manner during spermatogenesis. Here we show that in mice, the absence of a testis-specific, cytoplasmic polyadenylate [poly(A)] polymerase, TPAP, results in the arrest of spermiogenesis. TPAP-deficient mice display impaired expression of haploid-specific genes that are required for the morphogenesis of germ cells. The TPAP deficiency also causes incomplete elongation of poly(A) tails of particular transcription factor messenger RNAs. Although the overall cellular level of the transcription factor TAF10 is unaffected, TAF10 is insufficiently transported into the nucleus of germ cells. We propose that TPAP governs germ cell morphogenesis by modulating specific transcription factors at posttranscriptional and posttranslational levels.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kashiwabara, Shin-Ichi -- Noguchi, Junko -- Zhuang, Tiangang -- Ohmura, Ko -- Honda, Arata -- Sugiura, Shin -- Miyamoto, Kiyoko -- Takahashi, Satoru -- Inoue, Kimiko -- Ogura, Atsuo -- Baba, Tadashi -- New York, N.Y. -- Science. 2002 Dec 6;298(5600):1999-2002.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Applied Biochemistry, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12471261" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Apoptosis
;
Cytoplasm/enzymology
;
Female
;
Gene Expression Regulation, Developmental
;
Gene Targeting
;
In Situ Nick-End Labeling
;
Male
;
Mice
;
Mice, Inbred C57BL
;
Mutation
;
Nuclear Proteins/genetics/metabolism
;
Organ Size
;
Poly A/metabolism
;
Polynucleotide Adenylyltransferase/genetics/*metabolism
;
Protein Biosynthesis
;
RNA, Messenger/*metabolism
;
Spermatids/physiology
;
Spermatocytes/physiology
;
*Spermatogenesis
;
Spermatozoa/*physiology
;
Testis/*enzymology/metabolism
;
Transcription Factors/genetics/metabolism
;
Transcription, Genetic
;
mRNA Cleavage and Polyadenylation Factors/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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