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  • 1
    Electronic Resource
    Electronic Resource
    Implication of tissue transglutaminase and desmoplakin in cell adhesion mechanism in human epidermis (2000)
    Springer
    Molecular and cellular biochemistry 206 (2000), S. 57-65 
    Details
    ISSN: 1573-4919
    Keywords: acantholysis ; cystamine ; desmoplakin ; enalapril ; transglutaminase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The distribution patterns of both tissue and keratinocyte transglutaminases (TGase), as well as that of desmoplakin (DP), have been immunohistochemically investigated in human skin cultured in the absence or presence of cystamine and enalapril, two acantholytic agents. In the control samples, tissue TGase is predominantly expressed in lower layers of the epidermis and is located intercellularly. Conversely, in tissues cultured with cystamine or enalapril, a diffuse cytoplasmatic staining was observed. Similarly, DP, detected on the cell membrane in the control, shifts into the cytosol of the keratinocytes following treatment. The distribution pattern of the keratinocyte enzyme in the acantholytic epidermis was identical to that observed in the normal one. Since cystamine and enalapril are TGase inhibitors and DP was shown to act as a TGase substrate in vitro, we suggest that DP and tissue enzyme may participate in cell adhesion at the intraepidermal level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007006219215
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of carrot nuclear proteins that exhibit specific binding affinity towards conventional and non-conventional DNA methylation (2000)
    Springer
    Plant molecular biology 44 (2000), S. 659-673 
    Details
    ISSN: 1573-5028
    Keywords: DNA-binding proteins ; symmetric and asymmetric methylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA methylation is associated with transcriptional silencing in vertebrates and plants. In mammals, the effects of methylation are mediated by a family of methyl-CpG-binding proteins. In plants the mechanisms by which methylation represses transcription are still not clear. In this paper we describe protein factors in carrot nuclear extracts exhibiting specific affinities for conventional or non-conventional methylation acceptor sites. We characterized two classes of proteins: the first, dcMBP1 (Daucus carota methylated DNA-binding protein 1), shows high affinity for sequences containing 5-methylcytosine; the second, dcMBP2 (Daucus carota methylated DNA-binding protein 2), efficiently complexes sequences containing 5-methylcytosine in both CpXpX and CpXpG trinucleotides and shows much lower affinity for 5-methyl CpG dinucleotides. Both dcMBP1 and dcMBP2 are abundant proteins differing in molecular weight and binding features. Their activities are modulated during carrot vegetative cell growth and somatic embryo development. This is the first time that, in either plants or mammals, proteins exhibiting specific binding affinities for conventional or non-conventional DNA methylation have been shown. Based on these results, the possibility that both the extent and the context of the methylation might contribute to modulate gene expression is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026590323386
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    Paper (German National Licenses)
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