ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Mixtures of bovine serum albumin (BSA) and several low molecular weight phenolics were incubated and fractionated using G-50 Sephadex chromatography. Fractions corresponding to the protein and the possible phenolic-protein complexes and fractions corresponding to the free phenolics were collected, and their phenolic content was determined. Among the selected commercial phenolic standards tested (p-coumaric acid, p-hydroxybenzoic acid, protocatechuic acid, caffeic acid and (+)-catechin), the strongest BSA-binding affinity was demonstrated by 3,4-dihydroxy benzoic and cinnamic acids (protocatechuic acid and caffeic acid), whereas p-hydroxybenzoic acid did not interact with BSA. The methodology was also applied to a phenolic extract obtained from lentils containing p-coumaric acid, a p-coumaric acid derivative, (+)-catechin and procyanidins B3 [(+)-catechin-(4αÕ8)-(+)-catechin] and B1 [(-)-epicatechin-(4αÕ8)-(+)-catechin]. Interactions observed among the lentil phenolics and BSA were comparable to those observed among the commercial phenolic standards and BSA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2000.tb16060.x
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