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  • Immunofluorescence  (4)
  • taxonomy  (3)
  • Magnetism
  • 2000-2004  (3)
  • 1975-1979  (4)
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  • 1
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    A new species of Afrothismia (Burmanniaceae; tribe: Thismieae) from the western foothills of Mount Cameroon (2004)
    In:  Blumea - Biodiversity, Evolution and Biogeography of Plants (0006-5196) vol.49 (2004) nr.2/3 p.451
    Details
    Publication Date: 2015-03-06
    Description: Afrothismia foertheriana, a new species of Burmanniaceae (tribe: Thismieae) from the peripheral zone of the Onge Forest Reserve in Cameroon’s Southwest Province is described and illustrated. The papillose, multicellular floral trichomes, the tepal’s erose margins, the small, zygomorphic perianth mouth and the dull purplish brown coloration give A. foertheriana a distinctive appearance within the genus. The species is here assessed as being critically endangered.
    Keywords: Burmanniaceae ; Thismieae ; Afrothismia foertheriana ; Cameroon ; conservation ; taxonomy
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
    Format: application/pdf
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  • 2
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    A new species of Afrothismia (Burmanniaceae; tribe: Thismieae) from the western foothills of Mount Cameroon (2004)
    In:  Blumea: Biodiversity, Evolution and Biogeography of Plants vol. 49 no. 2/3, pp. 451-456
    Details
    Publication Date: 2024-01-12
    Description: Afrothismia foertheriana, a new species of Burmanniaceae (tribe: Thismieae) from the peripheral zone of the Onge Forest Reserve in Cameroon\xe2\x80\x99s Southwest Province is described and illustrated. The papillose, multicellular floral trichomes, the tepal\xe2\x80\x99s erose margins, the small, zygomorphic perianth mouth and the dull purplish brown coloration give A. foertheriana a distinctive appearance within the genus. The species is here assessed as being critically endangered.
    Keywords: Burmanniaceae ; Thismieae ; Afrothismia foertheriana ; Cameroon ; conservation ; taxonomy
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
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  • 3
    Electronic Resource
    Electronic Resource
    Qualitative and quantitative immunofluorescence studies of chloroplast ferredoxin (1977)
    Springer
    Planta 137 (1977), S. 279-286 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast ; Ferredoxin ; Immunofluorescence ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Antibodies were raised in rabbits against 2Fe−2S ferredoxin from N. tabacum L. The antibodies showed partial cross-reactivity in the double diffusion test with ferredoxins from Spinacia oleracea L., Petunia inflata Fries., P. axillaris Lam., Phaseolus vulgaris L., Chlamydomonas remhardii Dang. A complete cross-reaction was observed with ferredoxins from five other Nicotiana species, thus with this test it was impossible to discriminate between these ferredoxins. Therefore the following test was performed. Heterologous ferredoxin (i.e., ferredoxin other than from N. tabacum) was coupled covalently to Sepharose beads. Rabbit anti-N. tabacum-serum was then pre-incubated with this ferredoxin which resulted in complete abolition of cross-reactivity with free heterologous ferredoxin. However, the serum retained antibody activity against specific antigenic determinants of N. tabacum ferredoxin. When this serum was tested against ferredoxin purified from the hybrid: N. tabacum (♀)xN. glutinosa (♂) it gave a positive reaction. The relative content of maternal N. tabacum ferredoxin in the hybrid was estimated by using a fluorescent derivative of this specific antibody and estimating the cross-reactivity compared with that of artificial mixtures of pure N. tabacum and N. glutinosa ferredoxins. The hybrid contained 50% of maternal ferredoxin. This technique was also applied to ferredoxins of other species of Nicotiana and to the ferredoxin from the hybrid N. clevelandii (♀)xN. glutinosa (♂). We conclude that it provides a good test system for the study of the expression of chloroplast ferredoxin in Nicotiana hybrids in general.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388163
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  • 4
    Electronic Resource
    Electronic Resource
    The gonadotropic cells in the pituitary of the black molly, Mollienisia latipinna, and other teleosts, identified by the immunofluorescence technique in normal and androgen-treated animals (1976)
    Springer
    Cell & tissue research 167 (1976), S. 211-219 
    Details
    ISSN: 1432-0878
    Keywords: Teleosts ; Pituitary ; GTH ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using anti-carp-gonadotropic-γ-globulin, the indirect immunofluorescence technique was applied to sections of the pituitary of 12 teleost species. From the investigated species, the Poeciliinae, and especially the black molly, showed a distinct localization of fluorescent cells in the mesoadenohypophysis. Strong fluorescence was observed in the ventralmost region, containing the presumed gonadotropic cells; weak fluorescence was observed in the dorsal region, in the presumed thyrotropic cells. The possibility of an unspecific reaction with TSH-cells in the latter region is discussed. Treatment of male black mollies for 38 days with methyltestosterone resulted in a loss of fluorescence in the ventral region of the meso-adenohypophysis, whereas there was no decrease of the fluorescence in the dorsal region. The results support the hypothesis that the ventral basophils in the mesoadenohypophysis produce gonadotropic hormone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224328
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  • 5
    Electronic Resource
    Electronic Resource
    Immunofluorescence studies on gonadotropin releasing hormone (GRH) in the fore-brain and the neurohypophysis of the green frog, Rana esculenta L. (1976)
    Springer
    Cell & tissue research 168 (1976), S. 325-333 
    Details
    ISSN: 1432-0878
    Keywords: Gonadotropin Releasing Hormone ; Fore-brain ; Rana esculenta ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using antibodies against mammalian LH-RH, the double antibody immunofluorescence technique has been applied to serial cross sections of the brains of adult Rana esculenta. Immunoreactive material was found in perikarya of an unpaired nucleus in front of the preoptic recess. The axons of these perikarya also contain fluorescing material. They form a single bundle which passes under the preoptic recess, than splits into two tracts, one on either side of the optic chiasm. The two tracts reunite just before entering the median eminence. The axons end near the capillaries in the outer zone of the median eminence. The possibility of two separate centres for the stimulation of gonadotropic activity in the brains of anurans is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215310
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  • 6
    Electronic Resource
    Electronic Resource
    Re-examination of some species of the genus Geotrichum Link: Fr (2000)
    Springer
    Antonie van Leeuwenhoek 77 (2000), S. 71-81 
    Details
    ISSN: 1572-9699
    Keywords: DNA heterogeneity ; genome comparison ; Geotrichum ; taxonomy ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nutritional physiology and the growth rate of thirty-four strains representing species of Geotrichum without known teleomorph states were examined. From twenty-seven strains the mol% G+C were calculated from the DNA melting curves. The first derivatives of the melting curves of seven strains, including the type strain of Geotrichum clavatum, demonstrated the presence of two peaks, 12% away from each other; the remaining strains showed only a single broad peak. DNA homology values among strains of the former group were high, indicating their conspecificity. The strains of the latter group could be subdivided into six DNA homology groups, four of which could be identified with recognized species and two may represent novel taxa. A combined key of Geotrichum and its teleomorph states Galactomyces and Dipodascus is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002030518989
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  • 7
    Electronic Resource
    Electronic Resource
    Localisation of gonadotropin releasing hormone (GRH) in the forebrain and neurohypophysis of the trout (Salmo gairdneri) (1977)
    Springer
    Cell & tissue research 181 (1977), S. 163-168 
    Details
    ISSN: 1432-0878
    Keywords: Gonadotropin releasing hormone ; Forebrain ; Salmo gairdneri ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The double antibody immunofluorescence technique was applied to serial coronal and sagittal sections of the brains of the trout, Salmo gairdneri, using an antibody against mammalian LH-RH. Immunoreactive material was found in small perikarya, situated at both sides of the ventriculus communis in the area dorsalis pars medialis of the telencephalon. The axons of these perikarya, also containing immunoreactive material, do not form a tract, but run diffusely in a caudo-ventral direction towards the pituitary stalk. The ending of these fibres has not yet been established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219978
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