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  • Springer  (311)
  • 2000-2004  (126)
  • 1975-1979  (185)
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  • 1
    Electronic Resource
    Electronic Resource
    Metal substitution in transferrins: specific binding of cerium(IV) revealed by the crystal structure of cerium-substituted human lactoferrin (2000)
    Springer
    JBIC 5 (2000), S. 692-698 
    Details
    ISSN: 1432-1327
    Keywords: Metal substitution Cerium binding Lactoferrin Crystal structure Transferrin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. Proteins of the transferrin family play a key role in iron homeostasis through their extremely strong binding of iron, as Fe3+. They are nevertheless able to bind a surprisingly wide variety of other metal ions. To investigate how metal ions of different size, charge and coordination characteristics are accommodated, we have determined the crystal structure of human lactoferrin (Lf) complexed with Ce4+. The structure, refined at 2.2 Å resolution (R=20.2%, R free=25.7%) shows that the two Ce4+ ions occupy essentially the same positions as do Fe3+, and that the overall protein structure is unchanged; the same closed structure is formed for Ce2Lf as for Fe2Lf. The larger metal ion is accommodated by small shifts in the protein ligands, made possible by the presence of water molecules adjacent to each binding site. The two Ce4+ sites are equally occupied, indicating that the known difference in the pH-dependent release of Ce4+ arises from a specific protonation event, possibly of the His ligand in one of the binding sites. Comparing the effects of binding Ce4+ with those for the binding of other metal ions, we conclude that the ability of transferrins to accommodate metal ions other than Fe3+ depends on an interplay of charge, size, coordination and geometrical preferences of the bound metal ion. However, it is the ability to accept the six-coordinate, approximately octahedral, site provided by the protein that is of greatest importance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007750000157
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  • 2
    Electronic Resource
    Electronic Resource
    On the calculation of sugar concentration in flower nectar (1979)
    Springer
    Oecologia 41 (1979), S. 301-304 
    Details
    ISSN: 1432-1939
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary There are several sources of potential error in calculating the concentration or energy value of floral nectar. Errors resulting from confusing data become substantial with increasing concentration. The different methods of expressing sugar concentration are here clarified, and the correct methods of converting from one to the other are provided. Refractometers in use in field studies usually read on a weight per total weight basis; this is recommended as the mode of statement. The perils of oversimplifying conversions from this mode, as is often done, are pointed out.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00377434
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  • 3
    Electronic Resource
    Electronic Resource
    Technical Opportunities for International Collaborations by the U.S. Fusion Program (2000)
    Springer
    Journal of fusion energy 19 (2000), S. 65-79 
    Details
    ISSN: 1572-9591
    Keywords: Fusion energy ; international collaboration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This report was prepared by a Working Group at the request of the U.S. Department of Energy, Office of Fusion Energy Sciences in 1997. The report addresses technical opportunities for mutually beneficial collaboration between the United States and other international fusion research programs. A number of outstanding opportunities are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1012281826821
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  • 4
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    On the calculation of sugar concentration in flower nectar (1979)
    Springer
    Details
    Publication Date: 1979-08-01
    Print ISSN: 0029-8549
    Electronic ISSN: 1432-1939
    Topics: Biology
    Published by Springer
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    PAPER CURRENT
  • 5
    Electronic Resource
    Electronic Resource
    Membrane-associated echinocandin B deacylase of Actinoplanes utahensis: purification, characterization, heterologous cloning and enzymatic deacylation reaction (2000)
    Springer
    Journal of industrial microbiology and biotechnology 24 (2000), S. 173-180 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: echinocandin B deacylase; substrate specificity; evolution/technology; antifungal agent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aspergillus nidulans produces echinocandin B, a neutral lipopeptide. A deacylase from Actinoplanes utahensis catalyzes cleavage of the linoleoyl group from echinocandin B, a key step in generating a potential antifungal agent. Virtually all (99.8%) deacylase activity was cell-associated. The deacylase was salt-solubilized, heat-treated and purified to apparent homogeneity by a 3-step chromatographic procedure. The enzyme was a heterodimer consisting of 63- and 18-to-20-kDa subunit, optimally active at pH 6.0, and at 60°C with salt. The K m of the deacylase for echinocandin B was 50 μM and its V max was 14.6 μmol cyclic hexapeptide min−1 mg−1protein. The substrate specificity of the enzyme was broad with respect to both acyl and cyclic peptide analogues of echinocandin B. The two deacylase subunit genes were cloned and over-expressed in Streptomyces lividans. The recombinant deacylase was purified from the culture filtrate to apparent homogeneity by a 1-step chromatographic procedure. Using the recombinant deacylase, an enzymatic deacylation of immobilized echinocandin B resulted in the generation of cyclic hexapeptide at gram-level. Journal of Industrial Microbiology & Biotechnology (2000) 24, 173–180.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900796
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  • 6
    Electronic Resource
    Electronic Resource
    A Method for Architecting Product Platforms (2000)
    Springer
    Research in engineering design 12 (2000), S. 61-72 
    Details
    ISSN: 1435-6066
    Keywords: Key words:Product families – Product platforms, Product portfolio architecture.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract: Consider a group of products sharing common parts and assemblies. The products in question we call a product family, and the common elements, the platform. In this paper, we present a method for designing product platforms and the derived family that takes into consideration both the technical performance requirements as well as the cost of the product family. The design of a platform-based product family is formulated as a general optimization problem in which the advantages of designing a common platform must be balanced against the constraints of the individual product variants and constraints of the family as a whole. This optimization approach forms the basis for a practical implementation as an interactive, team-based negotiation model for designing a family of interplanetary spacecraft based on a common platform. The approach is used to consider and specify different subsystems that could be made common to all the missions. It is also used to evaluate the impact of those platform design decisions on the performance of the product family, and thus be able to select from among feasible platform designs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001630050024
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  • 7
    Electronic Resource
    Electronic Resource
    Crystal structure of [WI2(CO)2{P(OiPr)3}(η2-EtC2Et)] (2000)
    Springer
    Journal of chemical crystallography 30 (2000), S. 181-184 
    Details
    ISSN: 1572-8854
    Keywords: tungsten(II) ; diiodo ; dicarbonyl ; triisopropylphosphite ; 3-hexyne ; crystal structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract [WI2(CO)2{P(OiPr)3}(η2-EtC2Et)] crystallizes in the monoclinic space group P21/n, with a = 11.101(12), b = 16.272(18), c = 14.892(17) Å, β = 93.27(1), Z = 4. The geometry can be considered to be pseudo-octahedral, with the 3-hexyne ligand occupying one site, with two iodo-groups, and the P(OiPr)3 ligand completing the equational plane of ligands, with two trans-carbonyl groups occupying the axial sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009531014474
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  • 8
    Electronic Resource
    Electronic Resource
    Concentration Dependence of Thermoelectric Power in Aqueous Tetra-n-Butylammonium Hydroxide Using Hydrogen Electrodes (2000)
    Springer
    Journal of solution chemistry 29 (2000), S. 595-604 
    Details
    ISSN: 1572-8927
    Keywords: Thermoelectric power ; tetra-n-butylammonium hydroxide ; heat of transport ; enthalpy of transport ; entropy of transport ; transported entropy ; thermocell ; hydrogen electrode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Using a hydrogen-electrode thermocell with a temperature difference of 8 K, initialthermoelectric power εin has been determined in aqueous tetra-n-butylammoniumhydroxide solutions for concentrations ranging from 0.001 to 0.2 molal at meantemperatures of 25 and 35°C. Graphs of a function of εin vs. m 1/2/(1 + m 1/2) yieldintercepts and infinite-dilution limiting slopes as m 1/2 → 0. Using the value $$\overline{\overline S} _{{\text{OH}}}^{\text{o}} $$ = 68.8±0.3 J-K−1for the standard transported entropy of the hydroxideion, values for the enthalpy of transport of tetra-n-butylammonium hydroxide atinfinite dilution were determined to be 36.5±1.5 kJ-mol−1 at25° and 37.7±2.0kJ-mol−1 at 35°C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005150314762
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  • 9
    Electronic Resource
    Electronic Resource
    Book reviews (1975)
    Springer
    Transportation 4 (1975), S. 313-328 
    Details
    ISSN: 1572-9435
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00153581
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  • 10
    Electronic Resource
    Electronic Resource
    Preliminary orbit-determination method having no co-planar singularity (1977)
    Springer
    Celestial mechanics and dynamical astronomy 15 (1977), S. 137-160 
    Details
    ISSN: 1572-9478
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract It has long been recognized and demonstrated in the astrodynamic literature that three observations of angular position are not always sufficient to determine a preliminary orbit. One reason for this is due to the fact that as the plane of the observer's motion approaches the plane of the orbit of the observed object, the determination of the orbit of the object becomes indeterminant. Merely changing the coordinate system will not eliminate the inherent indeterminacy or singularity. When the observed object is moving in the same plane as the observer, their relative motion is described in two dimensions rather than three. The problem reduces to defining two components of position and two of velocity given only three angular measures and no solution is possible. Although this singularity is a rather old, albeit infrequently arising problem in celestial mechanics, it has received renewed interest due to the advent of satellite observatories that observe other spacecraft. In this new circumstance the plane of the observer's motion is rather frequently near the plane of the object (12% to 35% of the time) and the co-planar singularity becomes a subject that deserves additional attention. It is the purpose of this paper to develop a practical and simple method of orbit determination using four observations. This method also allows one to avoid the problem of multiple orbit-determination solution roots, and provides numerical indices that are useful in assessing the degree of indeterminacy in any given observer/object geometry. This paper does not dwell at length on the theory of orbital singularities, since they have been already treated in celestial mechanics literature. Instead, the emphasis is on the details of a new computational technique, which has been found to be computationally more efficient than previous four-observation methods, and which is unique in being formulated in the geocentric system and involves only one scalar quantity in the correction process. The equations for the new method are developed and a numerical example is presented that demonstrates the efficiency of the method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01228460
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