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  • Chemistry  (207)
  • Immunofluorescence  (4)
  • dihaploid  (3)
  • Magnetism
  • 2000-2004  (2)
  • 1975-1979  (52)
  • 1955-1959  (71)
  • 1950-1954  (89)
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Keywords
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Qualitative and quantitative immunofluorescence studies of chloroplast ferredoxin (1977)
    Springer
    Planta 137 (1977), S. 279-286 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast ; Ferredoxin ; Immunofluorescence ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Antibodies were raised in rabbits against 2Fe−2S ferredoxin from N. tabacum L. The antibodies showed partial cross-reactivity in the double diffusion test with ferredoxins from Spinacia oleracea L., Petunia inflata Fries., P. axillaris Lam., Phaseolus vulgaris L., Chlamydomonas remhardii Dang. A complete cross-reaction was observed with ferredoxins from five other Nicotiana species, thus with this test it was impossible to discriminate between these ferredoxins. Therefore the following test was performed. Heterologous ferredoxin (i.e., ferredoxin other than from N. tabacum) was coupled covalently to Sepharose beads. Rabbit anti-N. tabacum-serum was then pre-incubated with this ferredoxin which resulted in complete abolition of cross-reactivity with free heterologous ferredoxin. However, the serum retained antibody activity against specific antigenic determinants of N. tabacum ferredoxin. When this serum was tested against ferredoxin purified from the hybrid: N. tabacum (♀)xN. glutinosa (♂) it gave a positive reaction. The relative content of maternal N. tabacum ferredoxin in the hybrid was estimated by using a fluorescent derivative of this specific antibody and estimating the cross-reactivity compared with that of artificial mixtures of pure N. tabacum and N. glutinosa ferredoxins. The hybrid contained 50% of maternal ferredoxin. This technique was also applied to ferredoxins of other species of Nicotiana and to the ferredoxin from the hybrid N. clevelandii (♀)xN. glutinosa (♂). We conclude that it provides a good test system for the study of the expression of chloroplast ferredoxin in Nicotiana hybrids in general.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388163
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  • 2
    Electronic Resource
    Electronic Resource
    The gonadotropic cells in the pituitary of the black molly, Mollienisia latipinna, and other teleosts, identified by the immunofluorescence technique in normal and androgen-treated animals (1976)
    Springer
    Cell & tissue research 167 (1976), S. 211-219 
    Details
    ISSN: 1432-0878
    Keywords: Teleosts ; Pituitary ; GTH ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using anti-carp-gonadotropic-γ-globulin, the indirect immunofluorescence technique was applied to sections of the pituitary of 12 teleost species. From the investigated species, the Poeciliinae, and especially the black molly, showed a distinct localization of fluorescent cells in the mesoadenohypophysis. Strong fluorescence was observed in the ventralmost region, containing the presumed gonadotropic cells; weak fluorescence was observed in the dorsal region, in the presumed thyrotropic cells. The possibility of an unspecific reaction with TSH-cells in the latter region is discussed. Treatment of male black mollies for 38 days with methyltestosterone resulted in a loss of fluorescence in the ventral region of the meso-adenohypophysis, whereas there was no decrease of the fluorescence in the dorsal region. The results support the hypothesis that the ventral basophils in the mesoadenohypophysis produce gonadotropic hormone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224328
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  • 3
    Electronic Resource
    Electronic Resource
    Immunofluorescence studies on gonadotropin releasing hormone (GRH) in the fore-brain and the neurohypophysis of the green frog, Rana esculenta L. (1976)
    Springer
    Cell & tissue research 168 (1976), S. 325-333 
    Details
    ISSN: 1432-0878
    Keywords: Gonadotropin Releasing Hormone ; Fore-brain ; Rana esculenta ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using antibodies against mammalian LH-RH, the double antibody immunofluorescence technique has been applied to serial cross sections of the brains of adult Rana esculenta. Immunoreactive material was found in perikarya of an unpaired nucleus in front of the preoptic recess. The axons of these perikarya also contain fluorescing material. They form a single bundle which passes under the preoptic recess, than splits into two tracts, one on either side of the optic chiasm. The two tracts reunite just before entering the median eminence. The axons end near the capillaries in the outer zone of the median eminence. The possibility of two separate centres for the stimulation of gonadotropic activity in the brains of anurans is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215310
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  • 4
    Electronic Resource
    Electronic Resource
    Inheritance of genetic markers from two potato dihaploids and their respective parent cultivars (1978)
    Springer
    Euphytica 27 (1978), S. 681-688 
    Details
    ISSN: 1573-5060
    Keywords: Potato ; dihaploid ; marker genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the first inbred generation (I1) of cv. Black 4495 a dark green, slowly growing mutant (coded ds) was found, whereas the I1 of its self-compatible dihaploid, B16, comprised this ds mutant and in addition the mutants virescens (v) and yellow margin (ym). The occurrence of ds and ym might trace back to diploid S. phureja, one of the ancestors of Black 4495. No lethal mutants were observed in I1 of B16. Analysis of I1 of cv. Gineke revealed a simplex condition for virescence and either duplex or triplex heterozygosity for one lethal gene. On the other hand, the I1 of its dihaploid, G254, segregated for virescence and for three different lethal genes. It is shown that both in B16 and in G254 homozygosity of an S-bearing translocation causes early death of embryo and endosperm, thus preventing seed development. From this study it appeared that the three lethal genes from G254 affect germination rate of the seeds. The genotypes at 11 loci of B16 and G254 are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023703
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  • 5
    Electronic Resource
    Electronic Resource
    Genetics of self-compatibility in dihaploids of Solanum tuberosum L. 4. Linkage between an S-bearing translocation and a locus for virescens (1978)
    Springer
    Euphytica 27 (1978), S. 381-384 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; self-compatibility ; dihaploid ; linkage ; virescens ; translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three dihaploids of Solanum tuberosum (two self-compatible, one self-incompatible) were found to be heterozygous for a monogenic recessive virescent mutant. Intercrossing resulted in the expected 3 : 1 ratio only in crosses involving one self-compatible and one self-incompatible parent. Self-compatible x self-compatible matings produced F1's in which 6:1 was found. The same ratio was observed in the self progeny of the two self-compatible dihaploids. This significant deviation could be explained by assuming linkage (25% crossing-over) between v and an S-bearing translocation. This translocation causes self-compatibility in the dihaploids used and early lethality when homozygous.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043162
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  • 6
    Electronic Resource
    Electronic Resource
    Genetics of self-compatibility in dihaploids of Solanum tuberosum L. 5. The S-genotype of tetraploid potato cultivar gineke (1978)
    Springer
    Euphytica 27 (1978), S. 385-388 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; incompatibility ; dihaploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Investigations of the genetics of self-compatibility and self-incompatibility in dihaploids and diploid derivatives from cv. Gineke revealed the presence of S 1, S2 and S 3 at the S-locus of Gineke and in addition an S 1-allele on a translocation. By means of a complete tester set involving the S-alleles S 1, S2 and S 3 (all from Gineke) and S 4 (from Black 4495) it was demonstrated that some Gineke dihaploids were compatible with all six testers. This indicated a fourth S-allele in Gineke, which differs from those in the tester series and was therefore assigned S 5. Additional evidence was obtained from an analysis of F1's from crosses of two S 5-bearing dihaploids and one of the testers. So the S-genotype of cv. Gineke was identified as S 1S2S3S5/S1, the second S 1 being the S-allele on a translocated fragment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043163
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of the base-pairing content of four specific tRNAs by infrared spectroscopy (1977)
    New York : Wiley-Blackwell
    Biopolymers 16 (1977), S. 1735-1745 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Infrared spectroscopy was used for the determination of the base-pairing content of four specific tRNAs in deuterium oxide solution. Infrared spectra were obtained in the 1750-1550 cm-1 region at various temperatures ranging from about 15 to 90°C. Melting curves were constructed by plotting the molar extinction coefficient at ν = 1657 cm-1 versus temperature. These transition curves enabled us to determine the ranges of temperature which correspond to the ordered (partially double-stranded) or randomly coiled structure of the tRNA. For a set of wavenumbers the extinction coefficients at these temperatures were used for the calculation of the base-pairing content. The procedure employed here is based on a method described earlier by Thomas [(1969) Biopolymers 7, 325-334]. For the conditions selected for this investigation (Mg2+-free D2O-buffer; 0.01M tris-DCl, 0.015M NaCl, pD 7.5) the results of this determination agree within the limits of errors with the number of base pairs predicted by the cloverleaf model.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1977.360160810
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  • 8
    Electronic Resource
    Electronic Resource
    Über die Bildung von Verkrustungen in Spinnbadeindampfern der Viscosefaserindustrie (1952)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 24 (1952), S. 401-402 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Es wird gezeigt, daß die im Betriebe von Spinnbadeindampfern fallweise auftretenden, schwer entfernbaren Verkrustungen der Heizrohre im wesentlichen durch die Ausscheidung von Glauberit verursacht werden. Verschiedene Einflüsse, die die Bildung des Doppelsalzes begünstigen, werden aufgeklärt.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330240704
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  • 9
    Electronic Resource
    Electronic Resource
    Wirkungsweise und Anwendungsergebnisse der registrierenden StaubwaageVortrag auf der 7. Arbeitstagung des Fachausschusses für Staubtechnik im VDI am 2. April 1952 in Wiesbaden; vgl. den Tagungsbericht, diese Ztschr. 24, 463 [1952]. (1952)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 24 (1952), S. 505-508 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Nach einführender Betrachtung des Meßproblems hinsichtlich der zu verlangenden Meßgenauigkeit und den sich ergebenden Einzelforderungen werden die Bestandteile des Staubmeßgerätes, nämlich die Waage, die Staubabscheidungskammer, das Gebläse, das Hochspannungsgerät und die zum selbsttätigen Ablauf notwendige Automatik besprochen. Ferner wird über vergleichende Versuche mit einem optischen Staubmeßgerät berichtet.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330240906
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  • 10
    Electronic Resource
    Electronic Resource
    Die Berechnung von Wärmeaustauschern mit Hilfe dimensionsloser KennzahlenVorgetragen auf der Sitzung des Arbeitsausschusses „Wärmeaustauscher und Verdampfer“ des VDI-Fachausschusses Verfahrenstechnik am 26. März 1952 in Bad Kreuznach; vgl. a. diese Ztschr. 24, 363 [1952]. (1953)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 25 (1953), S. 221-227 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Die Ähnlichkeitstheorie hat den Weg gezeigt, die Gesetzmäßigkeiten des Wärmeüberganges mit Hilfe dimensionsloser Kennzahlen auszudrücken. Die Anzahl der zur eindeutigen Beschreibung eines Vorganges notwendigen Kenngrößen ist recht verschieden, sie ist z. B. besonders groß bei komplizierten Austauscherformen. Bei der Zusammenfassung der Einzelfaktoren zu Kennzahlen hat man gewisse Freiheiten, die man dazu benützen kann, die Gesetzmäßigkeiten bei der Berechnung von Wärmeaustauschern in einer möglichst zweckmäßigen Form darzustellen. An einem Beispiel wird gezeigt, daß die Rechnungen sich verhältnismäßig einfach durchführen lassen.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330250502
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