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  • Cytogenetics  (3)
  • Springer  (3)
  • American Geophysical Union (AGU)
  • American Physical Society (APS)
  • Blackwell Publishing Ltd
  • 2000-2004
  • 1980-1984  (3)
  • 1970-1974
  • 1965-1969
  • 1940-1944
Collection
Keywords
Publisher
  • Springer  (3)
  • American Geophysical Union (AGU)
  • American Physical Society (APS)
  • Blackwell Publishing Ltd
Years
  • 2000-2004
  • 1980-1984  (3)
  • 1970-1974
  • 1965-1969
  • 1940-1944
Year
  • 1
    Electronic Resource
    Electronic Resource
    Cytogenetic analysis of plants regenerated from colchicine-treated callus cultures of an interspecificHordeum hybrid (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 89-95 
    Details
    ISSN: 1432-2242
    Keywords: Cytogenetics ; Plant regeneration ; Introgressive breeding ; Callus culture ; Regenerates ; Barley ; Hordeum hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hybrids ofHordeum vulgare (HV) xH. jubatum (HJ) were synthesized for purposes of introgressive breeding, but were sterile and the recovery of pure diploid tillers by colchicine applications in vivo was difficult. Plant regeneration from colchicine-treated callus cultures of the hybrid (HV x HJ) was investigated as a means to produce high numbers of pure diploid, fertile intermediates. 10 of 50 plants regenerated in this manner exhibited variable chromosome numbers with means of approximately 37 (expected diploid number = 42). Cytological examinations of microsporogenesis in all such plants revealed a high incidence of bivalent formation at metaphase I (as compared to nearly complete asynapsis in the F1), but spindle and chromosome abnormalities in later meiotic stages led to complete sterility. Approximately 40% of HJ plants regenerated from colchicine-treated calli appeared to be pure tetraploids of high fertility. These techniques are hence useful for high frequency production of diploid or polyploid plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00745037
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  • 2
    Electronic Resource
    Electronic Resource
    Chromosomal variability in tissue cultures and regenerated plants of Hordeum (1980)
    Springer
    Theoretical and applied genetics 56 (1980), S. 101-112 
    Details
    ISSN: 1432-2242
    Keywords: Plant tissue culture ; Cytogenetics ; Plant breeding ; Barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spontaneous polyploidy, aneuploidy, and chromosomal rearrangements were observed in callus and suspension cultures of Hordeum vulgare, H. jubatum, and their interspecific hybrid. The extent to which each class of chromosomal variability was present in a culture depended upon differentiated state, age, and history. Cytological and isozymic analysis of subdivided callus cultures revealed spatial segregation of chromosomal variability. Cytogenetic analyses were performed to determine the expression of this in vitro chromosomal variability in corresponding regenerated plant tissues. A complete loss of polyploidy and a decrease in aneuploidy and chromosomal rearrangements were observed. Analyses of specific isozyme activities in regenerates suggested that a quantitative segregation of H. vulgare and H. jubatum genomes had occurred in tissue cultures of their interspecific hybrid. Possible uses of in vitro chromosomal variability for plant breeding and genetical studies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265080
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cytogenetic analysis of plants regenerated from colchicine-treated callus cultures of an interspecific Hordeum hybrid (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 89-95 
    Details
    ISSN: 1432-2242
    Keywords: Cytogenetics ; Plant regeneration ; Introgressive breeding ; Callus culture ; Regenerates ; Barley ; Hordeum hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hybrids of Hordeum vulgare (HV) x H. jubatum (HJ) were synthesized for purposes of introgressive breeding, but were sterile and the recovery of pure diploid tillers by colchicine applications in vivo was difficult. Plant regeneration from colchicine-treated callus cultures of the hybrid (HV x HJ) was investigated as a means to produce high numbers of pure diploid, fertile intermediates. 10 of 50 plants regenerated in this manner exhibited variable chromosome numbers with means of approximately 37 (expected diploid number = 42). Cytological examinations of microsporogenesis in all such plants revealed a high incidence of bivalent formation at metaphase I (as compared to nearly complete asynapsis in the F1), but spindle and chromosome abnormalities in later meiotic stages led to complete sterility. Approximately 40% of HJ plants regenerated from colchicine-treated calli appeared to be pure tetraploids of high fertility. These techniques are hence useful for high frequency production of diploid or polyploid plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276409
    Location Call Number Expected Availability
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    Paper (German National Licenses)
    Fulltext
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