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  • General Chemistry  (203)
  • Cell & Developmental Biology  (93)
  • Meteorology and Climatology  (40)
  • Nonmetallic Materials
  • 2000-2004  (89)
  • 1980-1984  (200)
  • 1975-1979  (79)
  • 1
    Electronic Resource
    Electronic Resource
    Protamine inhibits platelet derived growth factor receptor activity but not epidermal growth factor activity (1984)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 26 (1984), S. 205-220 
    Details
    ISSN: 0730-2312
    Keywords: PDGF ; EGF ; receptor ; oncogenes ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Protamine sulfate blocked 125I-PDGF binding to its specific physiological receptor on Swiss mouse 3T3 cells. Reduced 125I-PDGF binding in the presence of protamine sulfate correlated directly with a protamine sulfate dose-dependent decrease in the PDGF-dependent incorporation of [3H]-thymidine into 3T3 cells and a decreased PDGF-stimulated tyrosine-specific protein kinase activity in isolated membrane preparations of 3T3 cells. Protamine sulfate blocked 125I-PDGF binding to simian sarcoma virus transformed cells (SSV-NIH 3T3 and SSV-NPl cells) and to nontransformed cells in a manner qualitatively identical to unlabelled PDGF. In contrast, protamine sulfate enhanced the specific binding of 125I-EGF by increasing the apparent number of EGF receptors on the cell surface. The increase in 125I-EGF receptor binding was not prevented by cycloheximide nor by actinomycin D. Protamine sulfate did not affect 125I-EGF binding to membranes from 3T3 cells or the EGF-stimulated 3T3 cell membrane tyrbsinc specific protein kinase activity, suggesting that protamine sulfate may have exposed a population of cryptic EGF receptors otherwise not accessible. Protamine sulfate was fractionated into four active fractions by Sephadex G-50 gel filtration columns; the half maximum inhibition concentration of 125I-PDGF binding to 3T3 cells of protamines I and II (MW ∼ 11,000 daltons and 7,000 daltons, respectively) is ∼ 0.4 μM. Protamine II (MW ∼ 4,800 daltons) was equally active (half maximum inhibition concentration ∼ 0.4 μM); protamine IV (MW ∼ 3,300 daltons) was substantially less active (half maximum inhibition concentration ∼ 2.8 μM).These investigations have extended previous observations that protamine sulfate is a potent inhibitor of PDGF binding and establish that protamine sulfate blocks PDGF binding at the physiological receptor, preventing PDGF initiated biological activities. Protamine sulfate can be used as a reagent to separate the influence of PDGF and EGF on cells with high specificity and has been used to demonstrate that the receptors on simian sarcoma virus transformed 3T3 cells qualitatively respond identically to protamine sulfate as to unlabelled PDGF and are likely identical to those on nontransformed 3T3 cells.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240260402
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    Paper (German National Licenses)
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  • 2
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    Mesoscale Simulations of CRYSTAL-FACE 23 July 2002 Case (2004)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: A key objective of the Cirrus Regional Study of Tropical Anvils and Cirrus Layers - Florida Area Cirrus Experiment (CRYSTAL-FACE) is to understand the relationships between properties of tropical convective cloud systems and the lifecycle of the extended cirrus anvils they produce. We report here on a case study of 23 July 2002 where a line of land-based convective storms was generated between Lake Okeechobee and the Florida east coast as a result of complex interactions between lake and sea breeze fronts and outflow boundaries. A central goal of this study is to develop a description of convective input to the anvil system and to quantify the ongoing dynamical forcing of anvil processes by mesoscale and large-scale dynamics. This information is then used to force high-resolution cloud simulations with a model that explicitly resolves cloud microphysical processes (bin model) for study of cirrus anvil microphysical development.
    Keywords: Meteorology and Climatology
    Type: 14th International Conference on Clouds and Precipitation; Jul 19, 2004 - Jul 23, 2004; Bologna; Italy
    Format: text
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    NASA TECHNICAL REPORTS
  • 3
    Electronic Resource
    Electronic Resource
    A scanning electron microscopic study of differentiation of the digital pad in regenerating digits of the kenyan reed frog, Hyperolius viridiflavus ferniqueiContribution number 78 from the Amphibian Facility.Supported in part by a grant from the Muscular Dystrophy Associations, Inc. and NIH Grants HD08150 and RR 0572 (1977)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 153 (1977), S. 387-395 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Newly metamorphosed Kenyan reed frogs, Hyperolius viridiflavus ferniquei, are able to regenerate amputated digits. The terminal digital pad is also completely reformed. Differentiation of the regenerating digital pad was studied by scanning electron microscopy. External differentiation of the digital pad began late in the second postamputational week with the appearance of small patches of specialized epidermal cells on the ventral surface of the regenerating digit. The differentiation of the pad spread out radially until late in the fourth week, when its overall shape approximated that of the normal digital pad. The appearance of patches of digital pad epidermis on the ends of spike regenerates arising from the forearm was also confirmed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051530305
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  • 4
    Electronic Resource
    Electronic Resource
    The fate mapping of the eleventh and twelfth day mouse otocyst: An in vitro study of the sites of origin of the embryonic inner ear sensory structures (1978)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 157 (1978), S. 249-267 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An experiment was undertaken to determine which sensory structures of the mouse embryo inner ear developed from what portion of the mouse otocyst. Otocysts of gestation days 10, 11, 12 and 13 were divided by surgical dissection into six anatomical groups: dorsal, ventral, anterior, posterior, medial and lateral halves. They were organ cultured separately. After a period of ten days, the explanted tissues were harvested and processed histologically for microscopic analysis. The surgical control specimens fixed at the time of explanation were composed of undifferentiated ectodermal cells for tissues of gestation days 10, 11, and 12. Otocysts of gestation day ten showed no gross morphological differentiation. Otocysts of gestation days 11 and 12 showed, during the course of their subsequent growth, that the three semicircular ducts and their associated cristae developed from the dorsal and lateral halves. Only the anterior and posterior canals and cristae originated from the medial portion. The posterior half gave rise to the posterior crista and the anterior half provided for the development of the anterior and lateral cristae. The cochlear duct and its sensory epithelium developed in all the anatomical groups except the dorsal half. The utricle developed in the dorsal section of the middle third of the otocyst, while the utricular macula developed in the anterior half of the same section of the otocyst. The saccule and its macula differentiated from the ventral section of the middle third of the anterior half.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051570302
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  • 5
    Electronic Resource
    Electronic Resource
    Avian scale development. VI. Ultrastructure of the keratinizing cells of reticulate scales (1979)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 161 (1979), S. 111-121 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Reticulate scales develop as radial symmetrical anlagen, in contrast to scuttate scales which appear initially as “epidermal placodes.” Unlike scuttate scales whose outer and inner epidermal surfaces elaborate β-and α-type keratins, respectively, reticulate scales elaborate only one type of epidermal surface which has been reported to give an α-type, X-ray diffraction pattern. We find that, histologically and ultrastructurally, this surface differs from either epidermal surface of scuttate scales. The keratinizing cells become filled with long interweaving bundles of α-filaments which aggregate into rather homogeneous α-fibrils. Keratohyalin granules, which have been shown to be associated with other keratinizing regions in the bird, do not form during the keratinization of reticulate scale epidermis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051610107
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  • 6
    Electronic Resource
    Electronic Resource
    Visceral anatomy of the amphisbaenia (1980)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 163 (1980), S. 99-133 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Study of the visceral anatomy of 41 specimens of amphisbaenians representing 13 genera shows that they share a very distinct structure which differs from that found in either snakes or typical lizards. The left lung is large while the right is rudimentary or absent (unique); the kidneys are freely suspended in the coelom by a mesentery (unique); the spleen is usually embedded in the anterior end of the pancreas (as in snakes); the gall bladder lies in a notch in the liver, and the kidneys lie opposite each other (as in lizards). The distinctness of this pattern supports the recognition of the Amphisbaenia as a separate suborder of the Squamata.
    Additional Material: 28 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051630202
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  • 7
    Electronic Resource
    Electronic Resource
    The cytoarchitecture of the torus semicircularis in the red-eared turtle (1981)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 169 (1981), S. 207-223 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cytoarchitecture and neuronal morphology of the torus semicircularis in the red-eared turtle, Chrysemys scripta elegans, were examined in Nissl-stained and Golgi-impregnated material. The torus semicircularis begins in the caudodorsal mesencephalon and extends rostrally and laterally to end ventrally to the tectal ventricle. The torus semicircularis consists of a central nucleus and a laminar nucleus, which is interposed between the central nucleus and the ventricle.The central nucleus can be divided into two regions, a small, large-celled area, located dorsally, and a larger area of small spherical (6-17 μm), large spherical (18-25 μm), triangular (15-27 μm) and fusiform (10-26 μm) neurons. The small spherical cells have two dendritic patterns: “radiate” and “single.” The radiate pattern has a dorsoventral orientation, several secondary branches and few dendritic spines. These cells are usually located in the center of the central nucleus. The single pattern is oriented mediolaterally. This cell type is most often observed at the periphery of the central nucleus. These neurons have few secondary branches and dendritic spines. The large spherical neurons display two dendritic orientations: dorsoventral and mediolateral. All dendritic trees have numerous secondary branches and few dendritic spines. The triangular neurons exhibit primary dendrites projecting from the corners of the somata and have few secondary branches and dendritic spines.The fusiform neurons have a majority of their dendrites oriented mediolaterally, few secondary branches and a small number of dendritic spines.The laminar nucleus consists of several layers and three cell types: ovoid (9-15 μm), triangular (20-40 μm), and fusiform (20-40 μm). All neurons have few secondary dendritic branches and few dendritic spines. The dendrites of many neurons course perpendicularly to the long axis of the brainstem and encapsulate the central nucleus. Some ovoid and fusiform neurons display dendrites that enter the central nucleus.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051690207
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  • 8
    Electronic Resource
    Electronic Resource
    Morphology of the pineal organ in the salamander Ensatina eschscholtzi (1981)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 169 (1981), S. 191-206 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The pineal organ of Ensatina eschscholtzi, a terrestrial and secretive species of salamander of the family Plethodontidae, is a photoreceptive structure lying on the dorsal surface of the diencephalon. The pineal is flattened with a broad lumen and consists of three cell types: photoreceptors, supportive cells, and neurons. Pineal photoreceptors are typical vertebrate photoreceptors and possess outer segment formations which, however, are frequently contorted and disorganized. Sloughing of apical portions of outer segments and vesiculation along the lateral edges of outer segment membrane disks are consistently observed and presumed to represent mechanisms of outer segment membrane recycling. Photoreceptors have basal processes which synapse with neural dendrites. Synapses between photoreceptor basal processes are occasionally observed. All synapses are characterized by synaptic ribbon structures of variable number, size, and configuration. Dense-core vesicles are occasionally observed mingled with clear synaptic vesicles within photoreceptor basal processes. Supportive cells within the pineal function in phagocytosis and recycling of shed outer segment membrane material, and neurons are localized at the lateral margins of the organ. The latter send axons into the ipsilateral side of the dorsal diencephalon. The pineal organ of Ensatina shows marked variation in overall size (cell total), cell type proportions, absolute neuron number, and ratio of photoreceptor number to neuron number for individual pineals. None of these morphological parameters is correlated with body size, sex, or season, and it is assumed that such variability represents significant variation in photosensory capabilities. It is suggested that the pineal organ of Ensatina is a partially degenerate photoreceptive structure.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051690206
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  • 9
    Electronic Resource
    Electronic Resource
    The maxillary palp organs of a wood-boring beetle, Melittomma sericeum (coleoptera, lymexylonidae) (1975)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 147 (1975), S. 123-135 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The complex and conspicuous basket-like structure attached to the third segment of each maxillary palp of Melittomma sericeum males is densely covered with tactile hairs on its outer or convex surface and with thinwalled chemoreceptors on its inner or concave surface. In a living male the structure is highly mobile and is extended laterally and ventrally. It evidently serves to detect odors produced by the female.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051470202
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  • 10
    Electronic Resource
    Electronic Resource
    Smooth muscle cells in the rat testicular capsule: A developmental study (1975)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 147 (1975), S. 171-185 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This study of the postnatal development (from 1 to 60 days) of smooth muscle elements in the rat testicular capsule has demonstrated that while such elements are identifiable by light microscopy at 30 days, myocytes are present at birth as seen by electron microscopy. The differentiation of smooth muscle from birth to 30 days has been described, by which time it is of adult morphology and content. Perhaps significantly, it is at 30 days that the testis achieves a scrotal position, although sexual maturity does not occur until about 60 days. Presumably, at 30 days the testicular capsule of the rat is capable of the spontaneous contractions which are known to occur in the adult and which are assumed to aid the transport of non-motile spermatozoa from the testis to the epididymis.The presence of occasional striated muscle fibers in the rat testicular capsule as reported previously has not been confirmed by this investigation, although their possible origin is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051470205
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