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  • 2000-2004  (9)
  • 1985-1989  (8)
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  • 1
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In controlled-environment experiments, ascospores of both A-group and B-group Leptosphaeria maculans were able to infect leaves of oilseed rape and produce phoma leaf spot lesions at temperatures from 5 to 20°C and wetness durations from 8 to 72 h after inoculation. Lesions formed on leaves inoculated with B-group ascospores had few pycnidia and were darker, smaller and less noticable than the larger, pale grey lesions with many pycnidia produced by A-group ascospores. Lesions formed by A-group or B-group L. maculans on naturally infected winter oilseed rape experimental crops were similar to lesions produced by the two groups on inoculated plants. The greatest numbers of lesions were produced with a leaf wetness duration of 48 h and at temperatures of 15–20°C for both A-group and B-group ascospores. As leaf wetness duration and temperature decreased below the optimal values, the number of lesions decreased. The incubation period, estimated as the time from inoculation to the appearance of the first lesions (t1), or the time to the appearance of 50% of the lesions (t50), of B-group was often shorter than that of A-group L. maculans. As temperature decreased below 20°C, the length of the incubation period of both A-group and B-group L. maculans increased.
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  • 2
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In controlled environment experiments, sporulation of Pyrenopeziza brassicae was observed on leaves of oilseed rape inoculated with ascospores or conidia at temperatures from 8 to 20°C at all leaf wetness durations from 6 to 72 h, except after 6 h leaf wetness duration at 8°C. The shortest times from inoculation to first observed sporulation (l0), for both ascospore and conidial inoculum, were 11–12 days at 16°C after 48 h wetness duration. For both ascospore and conidial inoculum (48 h wetness duration), the number of conidia produced per cm2 leaf area with sporulation was seven to eight times less at 20°C than at 8, 12 or 16°C. Values of Gompertz parameters c (maximum percentage leaf area with sporulation), r (maximum rate of increase in percentage leaf area with sporulation) and l37 (days from inoculation to 37% of maximum sporulation), estimated by fitting the equation to the observed data, were linearly related to values predicted by inserting temperature and wetness duration treatment values into existing equations. The observed data were fitted better by logistic equations than by Gompertz equations (which overestimated at low temperatures). For both ascospore and conidial inoculum, the latent period derived from the logistic equation (days from inoculation to 50% of maximum sporulation, l50) of P. brassicae was generally shortest at 16°C, and increased as temperature increased to 20°C or decreased to 8°C. Minimum numbers of spores needed to produce sporulation on leaves were ≈25 ascospores per leaf and ≈700 conidia per leaf, at 16°C after 48 h leaf wetness duration.
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  • 3
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Relationships between the incidence and severity of brown foot rot and of pathogenic fungi, determined by diagnostic and quantitative PCR, were investigated during the growth of nine winter wheat crops in three cropping seasons. Microdochium nivale vars nivale and majus were the only brown foot rot pathogens present in significant amounts. Relationships between disease symptoms and amounts of pathogen DNA were often weak in early spring (when shoot-base symptoms are usually most difficult to ascribe to particular pathogens by visual examination) because of indistinct symptoms and small amounts of pathogen. Relationships were strongest during stem elongation. The amount of M. nivale in the tissues tended to decline in the summer as the plants matured, apparently disappearing partially from necrotic lesions to which it contributed, resulting in a weakened relationship between symptoms and pathogen DNA. Regression analyses of brown foot rot on amounts of M. nivale DNA for different wheat cultivars generally produced lines with similar slopes but were often most significant for the cultivar with most eyespot resistance (i.e. with least confounding eyespot) or most apparently genuine brown foot rot. DNA of Fusarium spp. was rarely present in amounts sufficient to quantify.
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  • 4
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Despite differences in climate and in timing of light leaf spot epidemics between Poland and the UK, experiments provided no evidence that there are epidemiological differences between populations of Pyrenopeziza brassicae in the two countries. Ascospores of Polish or UK P. brassicae isolates germinated on water agar at temperatures from 8 to 24°C. After 12 h of incubation, percentages of ascospores that germinated were greatest at 16°C: 85% (Polish isolates) and 86% (UK isolates). The percentage germination reached 100% after 80 h of incubation at all temperatures tested. The rate of increase in germ tube length increased with increasing temperature from 8 to 20°C but decreased from 20 to 24°C, for both Polish and UK isolates. Percentage germination and germ tube lengths of UK P. brassicae ascospores were less affected by temperature than those of conidia. P. brassicae produced conidia on oilseed rape leaves inoculated with ascospores or conidia of Polish or UK isolates at 16°C with leaf wetness durations from 6 to 72 h, with most sporulation after 48 or 72 h wetness. Detection of both mating types of P. brassicae and production of mature apothecia on leaves inoculated with mixed Polish populations suggest that sexual reproduction does occur in Poland, as in the UK.
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  • 5
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Diagnostic and quantitative polymerase chain reaction (PCR) provided clarification of the causes of symptoms and the extent of infection by eyespot (Tapesia spp.) and sharp eyespot (Rhizoctonia cerealis) on winter wheat at early growth stages. Disease assessments made before stem extension, when decisions to apply fungicides are usually made, often did not agree with the pathogen diagnoses using PCR, suggesting that such early visual diagnoses may be unreliable. Visual and PCR diagnoses made on stems in summer generally supported each other, but there were often discrepancies in relating disease severity to amounts of pathogen present when determined by regression analyses of incidence or severity of symptoms on amount of pathogen DNA. Mixed symptoms caused by different pathogens may sometimes have been confounded. Relationships between symptoms and DNA of eyespot pathogens were less clear on some cultivars, often those with least disease. Sharp eyespot symptoms had a stronger relationship to DNA of its pathogen. Significant regressions often accounted for a small percentage of the variance, suggesting either that pathogens not assayed were contributing to symptoms or that lesions were in some cases persisting longer into the season than pathogen DNA. The frequency of pathogen detection before stem extension was a poor predictor of the amounts of pathogen DNA measured later in the season.
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  • 6
    Publication Date: 1986-06-01
    Description: SummaryMultifactorial experiments on winter barley cv. Igri grown after potatoes were made from 1981 to 1983 on silty clay loam soils at Rothamsted. All tested combinations of seven factors, each at two levels: with and without autumn pesticide (aldicarb), two sowing dates (September or October), with and without a fungicidal seed treatment (‘Baytan’), with and without spring and summer fungicides, two amounts of nitrogen, two times of applying nitrogen and with and without a growth regulator (‘Terpal’). Growth, development, yield, nitrogen uptake, pests and diseases were monitored. Sowing in September, fungicide sprays in spring and summer, and the growth regulator had the largest mean benefits on grain yield (+0·80, +0·56 and +0·34 t/ha respectively). Some factors interacted with sowing date; thus aldicarb, the fungicide sprays in spring and summer and the later timing of N all increased yield more on the September-than on the October-sown barley. The larger yields on the September-sown plots were associated with more ears/m2 (978 v. 744) and, in spite of fewer grains per ear (17·8 v. 20·1), more grains per m2 (17·6 v. 14·7 × 103), but lighter grains (39·2 v. 42·3 mg). The largest yields each year (ca. 8.0–8.5 t/ha) were obtained from September-sown barley fully protected from pests and from pathogens in spring and summer and given N in April rather than in March.The aphid vectors of barley yellow dwarf virus were sufficiently common and infective in two of the three autumns to infect the September-sown barley sufficiently that their control by aldicar b enhanced yield. Nematodes, slugs and dipterous stem borers were not numerous enough to be damaging in any year. Mildew in autumn was controlled by the seed treatment, but effects on yield were inconsistent. Mildew in spring and summer was more abundant on the October-than on the September-sown barley; it was controlled by fungicide sprays, which increased yield significantly each year. Leaf blotch was more abundant on the September-sown barley.
    Print ISSN: 0021-8596
    Electronic ISSN: 1469-5146
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 7
    Publication Date: 1988-02-01
    Description: SummaryWinter wheat grown following potatoes on a sandy loam at Woburn in 1978–9, 1980–1 and 1981–2 was compared with that on a clay loam at Rothamsted in 1978–9 and 1980–1, and on a silty clay (alluvium) at Woburn in 1981–2. The cultivar was Hustler in the harvest years 1979 and 1981 and Avalon in 1982. On each soil in each year multifactorial experiments tested effects of combinations of six factors, each at two levels.The best 4-plot mean grain yield ranged from 89 to 11·1 t/ha during the 3 years; it was smaller on the sandy soil than on the clay soil in 1979, but larger on sand than on the clay in 1981 and 1982. Until anthesis the number of shoots, dry weight and N content of the wheat giving these best yields were less on sand than on clay. Unlike grain weight, straw weight was always less on sand.Sowing in mid-September instead of mid-October increased grain yield on clay in each year (by 0·4·0·7 t/ha) and increased yield on sand only in 1981 (by 1·6 t/ha). Early sowing always increased dry weight, leaf area, number of shoots and N uptake until May. The benefits were always greater on clay than on sand immediately before N fertilizer was applied in the spring and usually lessened later on both soils.Aldicarb as an autumn pesticide increased grain yield of early-sown wheat on both soils in 1981 by lessening infection with barley yellow dwarf virus. Aldicarb increased yield on clay in 1982; it also decreased the number of plant parasitic nematodes.Wheat on sand was more responsive to nitrogen in division, timing and amount than was wheat on clay. In 1979 yield of wheat on sand was increased by dividing spring N between March, April and May, instead of giving it all in April, and in 1982 by giving winter N early in February. In 1981 division and timing on sand interacted with sowing date. Yield of early-sown wheat given N late, i.e. in March, April and May, exceeded that given N early, i.e. in February, March and May, by 1·4 t/ha; single dressings given all in March or all in April also yielded less than the late divided dressing. Yield of later-sown wheat given all the N in April was at least 1·2 t/ha less than with all N given in March or with divided N. In all years treatments that increased yield usually also increased N uptake. Grain yield on clay was never affected by division or timing of spring N or by application of winter N. This was despite the fact that all treatments that involved a delay in the application of N depressed growth and N uptake in spring on both sand and clay. The mean advantage in N uptake following early application of spring N eventually reversed on both soils, so that uptake at maturity was greater from late than from early application. Increasing the amount of N given in spring from the estimated requirement for 9 t/ha grain yield to that for 12 t/ha increased yield in 1982, especially on sand. The larger amount of N always increased the number of ears but often decreased the number of grains per ear and the size of individual grains.Irrigation increased grain yield only on the sandy soil, by 1·1 t/ha in 1979 and by 07 t/ha in 1981 and 1982. The component responsible was dry weight per grain in 1979 and 1982, when soil moisture deficits reaching maximum values of 136 and 110 mm respectively in the 2 years developed after anthesis; the component responsible was number of ears/m2 in 1982 when the maximum deficit of 76 mm occurred earlier, in late May.
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  • 8
    Publication Date: 2002-09-01
    Description: The effects of supplying the fertilizer nitrogen (N) as a recommended quantity of ammonium nitrate or as a commonly used dose of poultry manure on yield of sugarbeet infected with Beet mild yellowing virus (BMYV) or Beet yellows virus (BYV) were studied in field experiments at IACR-Broom's Barn in 1990, 1991 and 1992. Three N fertilizer treatments comprising Zero (N0), standard rate of 110 kg N/ha (N1) and poultry manure equivalent to c. 300 kg/ha of available N (N2) were applied to plots which were uninoculated or were subsequently inoculated with either BMYV or BYV. Averaged over virus treatments, N1 increased sugar yields by 23% relative to N0: there was no further increase when N2 was applied. When averaged over N treatments, early virus yellows infection reduced the sugar yields by 23%. Generally there was no significant interaction between N supply and virus infection. There was no evidence that the large N supply could reduce the yield effect of virus yellows infection, as had previously been thought. Crops infected from late July produced similar yields to uninoculated controls. The main effect of virus yellows was to reduce the efficiency of radiation conversion even when account was taken of the light intercepted by yellow foliage. Whilst the N2 treatment helped to maintain a green leaf cover throughout the season on virus yellows infected crops, it had no effect on virus replication. Beet processing quality was impaired by increasing the N supply and by virus infection, but again there were generally no significant interactions between infection and N rate.
    Print ISSN: 0021-8596
    Electronic ISSN: 1469-5146
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  • 9
    Publication Date: 1986-12-01
    Description: SUMMARYWinter wheat cv. Avalon was sown in autumn 1981, 1982 and 1983 on a clay loam soil following two cereal crops. Multifactorial experiments tested the effects of combinations of the following eight factors, each at two levels: rotation, sowing date, timing of nitrogen, amount of nitrogen, growth regulator, pesticide, spring fungicide and summer fungicide.The best 16-plot mean grain yields in 1982–4 were respectively 8·7, 10·2 and 11·1 t/ha. Rotation had the largest effect on grain yield. Wheat following barley was severely infected with take-all and yielded, on average over 3 years, 2·2 t/ha less than wheat following oats. Take-all was more severe on wheat sown in mid-September than in mid-October; its effects on yield were lessened by early timing of N in 1982. Take-all decreased growth and N uptake mainly after anthesis, and also number of ears and dry weight per grain. Sowing in mid-September compared with mid-October decreased yield of wheat after barley by an average of 0·8 t/ha because take-all was more severe. Early sowing had negligible effects on grain yield of wheat after oats, but increased straw dry weight by 1·1 t/ha.Spring fungioide increased yield by an average of 0·3 t/ha. Effects were larger after barley than after oats, associated with a greater incidence of eyespot after barley. Summer fungioide increased yield by an average of 0·3 t/ha. Foliar diseases were slight in all 3 years. Fusarium ear blight and sharp eyespot were prevalent in 1982 and were not well controlled by the fungioide treatments. Fungicide temporarily decreased the incidence of some components of the mioroflora on the ears. Pesticide increased grain yield of wheat after oats only in 1984, when aphids on the ears were numerous. Aphids were present on early-sown plots in all three autumns but there was little barley yellow dwarf virus infection even without pesticide. Pesticide always decreased the number of nematodes after harvest to fewer than present before sowing. Populations never approached levels expected to affect yield.Early N application (main application early March) resulted in a larger grain yield in 1982 than N applied a month later. In 1983 and 1984 grain yield and N uptake by the grain were greater with the late application, especially when wheat was sown early. The soil contained more mineral N in the autumn of 1982 and 1983 than in 1981. Straw weight was always greater with early than with late application. Increasing the amount of N applied from 163 to 223 kg/ha increased N uptake by 40 kg/ha and grain yield by 0·5 t/ha after oats and by 0·6 t/ha after barley. N uptake in grain plus straw by the best yielding crops ranged from 205 kg/ha in 1982 to 246 kg/ha in 1984.Chlormequat applied at the start of stem extension shortened the stems at maturity by 2 cm each year. In 1984 it inoreased yield of early-sown wheat by 0·3 t/ha and also decreased lodging, which did not occur in the first 2 years.
    Print ISSN: 0021-8596
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  • 10
    Publication Date: 2002-11-01
    Description: Results from field experiments with mobile pests and air-borne pathogens are subject to bias as a result of inter-plot interference. Serially balanced designs (SBDs) allow interference to be estimated but other designs may be better for decreasing such effects. To investigate this, systematic replicated designs, comparing sprays applied at different times to control powdery mildew of spring barley, were sited in 2 years alongside SBDs testing similar treatments. Yields of grain and assessments of mildew on the leaves were analysed. Results from the balanced designs provided strong evidence of interference in both years but not in a third (when the systematic design was omitted). Estimates of treatment effects from the systematic designs were often, but not consistently, greater than corresponding estimates from the SBDs. A method of analysis from Draper & Guttman (1980) was adapted to produce estimates of the differences between treatments as if applied to all plots of an experiment; this showed larger differences between treatments than the conventional analysis in 1975 and 1976 (when there was appreciable interference), but failed in 1977 when interference was slight. This method fails when applied to the systematic designs; SBDs (which are a subset of all designs in randomized blocks) are probably optimal for this type of analysis. The difficulties of analysing data in the form of percentages or proportions (with consequent non-orthogonality) are discussed.
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    Electronic ISSN: 1469-5146
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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