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  • PEG  (1)
  • real time  (1)
  • Springer  (2)
  • American Society of Hematology
  • Frontiers Media
  • Springer Nature
  • Wiley
  • ZBW - Deutsche Zentralbibliothek für Wirtschaftswissenschaften, Leibniz-Informationszentrum Wirtschaft Kiel, Hamburg
  • 2000-2004  (1)
  • 1985-1989  (1)
Collection
Publisher
  • Springer  (2)
  • American Society of Hematology
  • Frontiers Media
  • Springer Nature
  • Wiley
  • +
Years
  • 2000-2004  (1)
  • 1985-1989  (1)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 124 (1985), S. 65-70 
    ISSN: 1615-6102
    Keywords: Protoplast ; Fusion ; Fluorescence ; PEG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two fluorescent compounds, scopoletin and carboxyfluorescein, have been used to label both tissue culture and leaf mesophyll cells and protoplasts. The compounds localized within the vacuoles of cells in approximately 15 hours. They remained in the vacuole during cell wall digestion, and fluorescence was observable for several hours after protoplast release. A one day pulse of these fluorescent labels had no deleterious effect on the growth of cells or protoplasts. When morphologically indistinguishable protoplasts were labeled and treated with polyethylene glycol, multicolored fluorescent fusion products were observable. These fluorescent labels provide a convenient method for selection of heterokaryon fusion products of whole plant and tissue culture cell protoplasts.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Mathematical geology 32 (2000), S. 159-185 
    ISSN: 1573-8868
    Keywords: El Niño—Southern Oscillation ; real time ; data analysis ; time series ; animation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Mathematics
    Notes: Abstract Traditional undergraduate education in earth sciences does not emphasize data acquisition, analysis, or assessment. However, arrival of the information age dictates that earth sciences graduates be imbued with fundamental skills to organize, evaluate and process large data sets. Fortunately, the proliferation of remotely sensed data and its availability via the Internet provides many opportunities for earth science educators to meet these needs. Exercises to introduce students to data analysis have been designed utilizing data from the Tropical Atmosphere–Ocean (TAO) Array and the 1997–1998 El Niño episode in the tropical Pacific Ocean. The TAO Array is a grid of 69 buoys moored across the equatorial Pacific Ocean (8°N to 8°S and 95°W to 143°E) recording environmental data relevant to El Niño—Southern Oscillation (ENSO) processes. Data from the TAO Array is available in near-real-time (http://www.pmel.noaa.gov/toga-tao/realtime.html) or as archived ASCII files (http://www.pmel.noaa.gov/toga-tao/data-delivery.html) providing daily (sometimes hourly) records of environmental parameters for each buoy in the grid. Student exercises in data analysis begin with downloading data from buoy locations, parsing the data into spreadsheets, and organizing data by environmental parameter into yearly and monthly data sets. Analyses of reconstructed data include calculations of long-term averages of environmental parameters, seasonal climatologies, monthly climatologies and calculation of long-term, seasonal, and monthly anomalies. Finally, monthly anomaly maps produced by students are loaded sequentially into GIF-animation software to create time-series images illustrating the progress and development of the 1997–1998 El Niño event.
    Type of Medium: Electronic Resource
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