ISSN:
1573-5079
Keywords:
ELISA
;
fructose-1,6-bisphosphatase
;
photosynthesis
;
quantitative determination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bisphosphatase content. However, this method is not sufficiently specific when the total protein is higher than 5 μg/cm3; so, despite its lower sensitivity, in these circumstances a competitive technique is more suitable. Thus photosynthetic fructose-1,6-bisphosphatase can be measured without interferences from the gluconeogenic cytosolic enzyme of the photosynthetic cell or from a non-specific phosphatase present in the chloroplast.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00032710
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